Iridoid Glucosides from the Flowers of<i>Barleria lupulina</i>

Suksamrarn, Sunit; Wongkrajang, Kanjana; Kirtikara, Kanyawim; Suksamrarn, Apichart

doi:10.1055/s-2003-43223

Cited by 30 publications

(5 citation statements)

References 11 publications

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“…Thus, HAE-BL selectively activates Nrf2-mediated transcription. In contrast, we did not detect induction of Nrf2 target genes with two compounds previously isolated from Barleria lupulina and which we specifically identified in HAE-BL: the iridoid glycoside, shanzhiside methyl ester (Kanchanapoom et al, 2001; Kim et al, 2015; Suksamrarn et al, 2003), and the phenolic glycoside, poliumoside (Kanchanapoom et al, 2001) (Figure 1A). We also investigated induction of Nrf2 target genes by HAE-BL at 4 hours.…”

Section: Resultscontrasting

confidence: 71%

“…In particular, the Thailand Ministry of Public Health has categorized BL as an essential medicinal plant for primary healthcare (Wanikiat et al, 2008). Phytochemical studies on this plant have focused on a number of iridoid glycosids as main components (Kanchanapoom et al, 2001; Kim et al, 2015; Suksamrarn et al, 2003). However, the compounds in BL that provide medicinal benefits towards reducing inflammation and the vascular complications of diabetes have not been defined.…”

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory activity of Barleria lupulina: Identification of active compounds that activate the Nrf2 cell defense pathway, organize cortical actin, reduce stress fibers, and improve cell junctions in microvascular endothelial cells

Senger

Hoang

Kim

et al. 2016

Journal of Ethnopharmacology

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Ethnopharmacological relevance Hot aqueous extracts of the plant Barleria lupulina (BL) are used for treating inflammatory conditions and diabetic vascular complications. Aim of the Study The goal was to identify active compounds in hot aqueous extracts of BL (HAE-BL) that are consistent with a role in reducing inflammation and reducing the vascular pathology associated with diabetes. In particular, we examined activation of the Nrf2 cell defense pathway because our initial findings indicated that HAE-BL activates Nrf2, and because Nrf2 is known to suppress inflammation. Activation of Nrf2 by HAE-BL has not been described previously. Materials and Methods Human endothelial cells, real-time PCR, western blotting, cytoskeletal analyses, and assay-guided fractionation with HPLC were used to identify specific compounds in HAE-BL that activate the Nrf2 cell defense pathway and reduce markers of inflammation in vitro. Results HAE-BL potently activated the Nrf2 cell defense pathway in endothelial cells consistent with its traditional use and reported success in reducing inflammation. Assay guided fractionation with HPLC identified three alkyl catechols: 4-ethylcatechol, 4-vinylcatechol, and 4-methylcatechol, that are each potent Nrf2 activators. In addition to activating Nrf2, HAE-BL and akyl catechols each profoundly improved organization of the endothelial cell actin cytoskeleton, reduced actin stress fibers, organized cell-cell junctions, and induced expression of mRNA encoding claudiin-5 that is important for formation of endothelial tight junctions and reducing vascular leak. Conclusions HAE-BL contains important alkyl catechols that potently activate the Nrf2 cell defense pathway, improve organization of the endothelial cell cytoskeleton, and organize tight cell junctions. All of these properties are consistent with a role in reducing inflammation and reducing vascular leak. Because activation of the Nrf2 cell defense pathway also prevents cancers, neuro-degeneration, age-related macular degeneration, and also reduces the severity of chronic obstructive pulmonary disorder and multiple sclerosis, HAE-BL warrants additional consideration for these other serious disorders.

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Section: Resultscontrasting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of Barleria lupulina: Identification of active compounds that activate the Nrf2 cell defense pathway, organize cortical actin, reduce stress fibers, and improve cell junctions in microvascular endothelial cells

Senger

Hoang

Kim

et al. 2016

Journal of Ethnopharmacology

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“…Several chemical constituents have been identified through GC-MS analysis of the ethanolic extract (Table 3). Some compounds viz., phenol,2,4,bis (1,1-dimethylethyl)-tetradecanoic acid, 12-methyl-, methyl ester, hexadecanoic acid, methyl ester, phytol and octadecanoic acid have also been reported by Omulokoli et al [22] and Namuli et al [23]. Cyclobutane, 1,1-dimethyl-2-octyl, 2-hexyl-1-octanol, 1, 2-henzenedicarboxylic acid, mono (2-ethylhexyl) ester and 1-hentetracontanol have been reported in essential oil of B. lupulina by Sarmad et al [24].…”

Section: Discussionmentioning

confidence: 83%

Phytochemical Analysis and Antibacterial and Cytotoxic Properties of Barleria lupulina Lindl. Extracts

Kumari¹,

Dubey²

2016

J Plant Pathol Microbiol

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(India) during the month of June 2014, and identified by the experts of the department (specimen identification No Bot. and micro/199). The plant leaves were washed with running tap water to remove the adhered dust, dirt and unwanted particles from their surfaces. The leaves were shade dried at room temperature for 15 days and separately homogenized in domestic blender to get fine powders. The powders were stored in airtight container at room temperature for further studies. Bacterial cultures Five standard human enteric pathogenic bacteria viz. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 27853, Pseudomonas aeruginosa ATCC 25922, Klebsiella pneumoniae MTCC 432 and Salmonella typhi MTCC 733 were procured from the Laboratory of the Departmental of Botany & Microbiology, Gurukula Kangri University. All the pathogens were sub cultured on nutrient agar slants and preserved at 4ºC for further study.

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“…It comprises of several medicinal properties including antibacterial and anti-inflammatory activities [ 15 ], anticancerous [ 33 ], anti-HSV-2 activities [ 35 , 36 ], anti-clastogenic, anti-tumor [ 28 , 29 ], antiarthritic [ 15 ], anti-diabetic, immunomodulatory [ 17 ]. Suksamrarn et al [ 27 ] reported that several iridoid glucosides, bataine and alkaloids have also been reported from B. lupulina plant. Traditionally, leaves of this plant are used to treat snake bites, dog bites, swelling, bleeding wounds and rheumatism.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial, antioxidant and Immuno-modulatory properties in extracts of Barleria lupulina Lindl.

Kumari

Kumar

et al. 2017

BMC Complement Altern Med

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BackgroundAntibacterial, immunomodulatory and antioxidant properties of aerial parts of Barleria lupulina Lindl were investigated in the present communication.MethodsThe antibacterial, antioxidant and immunomodulatory properties of B. lupulina (methanol soluble leaf and stem extracts) was analyzed by minimum inhibitory concentration, total phenolic contents, DPPH radical scavenging activity, determination of toxicity, hemagglutination antibody titre, delayed type hypersensitivity and neutrophil adhesion test, respectively.ResultsMethanol soluble leaf extract (MLE) contains more soluble bioactive compounds inhibiting the growth of five bacterial pathogens viz., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella typhi even at MICs of 1.25 and 2.5 mg/mL. Aqueous stem extract (ASE) was least effective while MLE was highly effective in inhibiting the growth and survival of bacterial pathogens. While testing the effect of these extracts in animal model, no mortality of albino rats was recorded by using MLE and MSE at the concentrations from 200 to 600 mg/kg of their body weight. The MLE showed significant result in agglutination reaction and induced paw edema volumes when compared with untreated group (control). Both MLE and MSE extracts significantly increased neutrophil adhesion with increase in doses of extracts. MLE was found to have more potent immune-stimulant properties than the MSE. High phenolic contents were found in MSE while lowest IC50 values were found in MLE in term of DPPH radical scavenging activity.ConclusionsMethanol soluble leaf and stem extract of Barleria lupulina contains antibacterial, antioxidants and immunomodulating phytochemical compounds that was effective for antibacterial, antioxidant and immunomodulatory properties. These may be used as synthetic drug.

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Iridoid Glucosides from the Flowers ofBarleria lupulina

Cited by 30 publications

References 11 publications

Anti-inflammatory activity of Barleria lupulina: Identification of active compounds that activate the Nrf2 cell defense pathway, organize cortical actin, reduce stress fibers, and improve cell junctions in microvascular endothelial cells

Anti-inflammatory activity of Barleria lupulina: Identification of active compounds that activate the Nrf2 cell defense pathway, organize cortical actin, reduce stress fibers, and improve cell junctions in microvascular endothelial cells

Phytochemical Analysis and Antibacterial and Cytotoxic Properties of Barleria lupulina Lindl. Extracts

Antibacterial, antioxidant and Immuno-modulatory properties in extracts of Barleria lupulina Lindl.

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