Antibacterial, antioxidant and Immuno-modulatory properties in extracts of Barleria lupulina Lindl.

Abstract

BackgroundAntibacterial, immunomodulatory and antioxidant properties of aerial parts of Barleria lupulina Lindl were investigated in the present communication.MethodsThe antibacterial, antioxidant and immunomodulatory properties of B. lupulina (methanol soluble leaf and stem extracts) was analyzed by minimum inhibitory concentration, total phenolic contents, DPPH radical scavenging activity, determination of toxicity, hemagglutination antibody titre, delayed type hypersensitivity and neutrophil adhesion test, respectively.ResultsMethanol soluble leaf extract (MLE) contains more soluble bioactive compounds inhibiting the growth of five bacterial pathogens viz., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella typhi even at MICs of 1.25 and 2.5 mg/mL. Aqueous stem extract (ASE) was least effective while MLE was highly effective in inhibiting the growth and survival of bacterial pathogens. While testing the effect of these extracts in animal model, no mortality of albino rats was recorded by using MLE and MSE at the concentrations from 200 to 600 mg/kg of their body weight. The MLE showed significant result in agglutination reaction and induced paw edema volumes when compared with untreated group (control). Both MLE and MSE extracts significantly increased neutrophil adhesion with increase in doses of extracts. MLE was found to have more potent immune-stimulant properties than the MSE. High phenolic contents were found in MSE while lowest IC50 values were found in MLE in term of DPPH radical scavenging activity.ConclusionsMethanol soluble leaf and stem extract of Barleria lupulina contains antibacterial, antioxidants and immunomodulating phytochemical compounds that was effective for antibacterial, antioxidant and immunomodulatory properties. These may be used as synthetic drug.

Optimizing Conditions for Microwave-Assisted Extraction of Polyphenolic Content and Antioxidant Activity of Barleria lupulina Lindl.

“…The species belongs to the Acanthaceae family that comprises 300 species [ 7 ]. It is commonly known as the hophead philippine violet and by various local names, such as Penawar seribu bisa (Malaysia) [ 8 ], Landik (Indonesia) [ 9 ], Sa-let-pangpon, Chong-ra-ar (Thailand) [ 10 ], Kanta vishalyakarni (India), and Neel saireyak (Sanskrit) [ 11 ]. Traditionally, B. lupulina was used to treat several diseases, including treating snake bites, dog bites, swelling, bleeding wounds, rheumatism, herpes simplex, and herpes zoster [ 10 , 11 ].…”

“…It is commonly known as the hophead philippine violet and by various local names, such as Penawar seribu bisa (Malaysia) [ 8 ], Landik (Indonesia) [ 9 ], Sa-let-pangpon, Chong-ra-ar (Thailand) [ 10 ], Kanta vishalyakarni (India), and Neel saireyak (Sanskrit) [ 11 ]. Traditionally, B. lupulina was used to treat several diseases, including treating snake bites, dog bites, swelling, bleeding wounds, rheumatism, herpes simplex, and herpes zoster [ 10 , 11 ]. Studies have documented that B. lupulina has medicinal values, including anti-inflammation [ 12 ], antiviral [ 13 , 14 ], antibacterial [ 11 , 15 , 16 ], antidiabetic [ 17 ], cytotoxic [ 18 , 19 ], antidiuretic, and antiarthritic activity [ 20 , 21 ].…”

“…Traditionally, B. lupulina was used to treat several diseases, including treating snake bites, dog bites, swelling, bleeding wounds, rheumatism, herpes simplex, and herpes zoster [ 10 , 11 ]. Studies have documented that B. lupulina has medicinal values, including anti-inflammation [ 12 ], antiviral [ 13 , 14 ], antibacterial [ 11 , 15 , 16 ], antidiabetic [ 17 ], cytotoxic [ 18 , 19 ], antidiuretic, and antiarthritic activity [ 20 , 21 ]. Several studies have also shown that the species possesses highly potential antioxidants and bioactive compounds used in treatments of various diseases and health issues [ 10 , 15 , 19 , 22 , 23 ].…”

Prenylated flavonoid-enriched fraction from Maclura tinctoria shows biological activity against Staphylococcus aureus and protects Galleria mellonella larvae from bacterial infection

“…Plant resources can also be sources of compounds with antimicrobial properties. Several studies on plant extract libraries have been established, and the results show the importance of this strategy for the discovery and development of new drugs [5, 6]. Coumarin derivatives have displayed activity against S. aureus and Bacillus anthracis DNA helicases [7].…”

Evaluation of antioxidant potential of alcoholic stem bark extracts of Bauhinia variegata Linn.

“…The extracts were then dried and weighed, and yield was calculated using the formula: yield % = X/Y ×100, where X = weight of the beaker with dried drug-weight of the beaker, and Y = total amount of the dried drug. Kumari et al (2017). Methods for antioxidant activity DPPH free radical scavenging activity: The free -radical scavenging activity of alcoholic stem bark extract was measured by decrease in the absorbance (Nuraniye et al, 2017).…”