Iophenoxic acid derivatives as markers of oral baits to wildlife

Abstract

The bait-marker iophenoxic acid (IPA) and its derivatives are increasingly used for evaluating and optimizing the cost-effectiveness of baiting campaigns on wildlife, particularly on game species such as the wild boar. We aimed to determine whether concentrations of the three main IPA derivatives ethyl, methyl and propyl-IPA measured on thoracic liquid extracts (TLE) of hunted wild boars may be representative of two exposure doses, 40 and 200 mg, from 20 to 217 days after ingestion. Then we developed a method of detection of the three IPA derivatives by LC/ESI-MS-MS in muscle and liver to evaluate the suitability of these two other tissues for monitoring the marked bait consumption and for measuring available residues in the meat of marked animals. Three semi-captive wild boars received 40 mg of each IPA derivative, three received 200 mg, and three, as controls, did not receive IPA. Blood serum was sampled 20, 197 or 217 days after IPA exposure according to animals and to the derivative. Wild boars were shot by gun after the different times of serum sampling times, and TLE, muscle and liver were sampled. Our results suggest that TLE is not a relevant tissue for quantitatively expressing IPA exposure. Due to interference, no analytical method was validated on TLE containing digestive material. On the other hand, quantifications in the muscle and particularly in the liver could discriminate wild boars that had ingested the two IPA doses from 20 days until 7 months after exposure, especially for the two long term markers ethyl and propyl-IPA. So IPA quantifications in the liver sampled on hunted animals appear to be a reliable tool for monitoring bait consumption in the field at a large scale. Nevertheless, whatever the ingested dose, ethyl- and propyl-IPA concentrations measured in the muscle and the liver of tested animals until 217 days after exposure, remained higher than 0.01 mg/kg, the Maximal Residue Limit (MRL) is recommended for molecules for which no toxicological data are available. Based on the range of IPA residues available in these two tissues, implications for humans consuming marked animals are discussed.

Controlling of CSFV in European wild boar using oral vaccination: a review

“…During initial field trials, tetracycline was used ( Kaden et al, 2000 ). However, bait markers efficient in this species (i.e., tetracycline, iophenoxic acid, rhodamine) are supposed at risk for human health, since wild boar are hunted and consumed by people ( Ballesteros et al, 2013 ; Sage et al, 2013 ) and subsequently increase the overall cost of vaccination, which compromises the use of chemical markers at a large scale in natural populations ( EFSA, 2008 ; Anses, 2012 ). Beef tallow which was used in the original bait, was removed from the bait matrix because regulatory requirements limited the use of certain bait materials (e.g., products derived from terrestrial animals; tissues that may transmit spongiform encephalopathy).…”