Ionophoretic properties of ferutinin

Zamaraeva, Maria; Hagelgans, Albert; Abramov, Andrey Y.; Ternovsky, Vadim I.; Merzlyak, Petr G.; Tashmukhamedov, B.A.; Saldkhodzjaev, Ashraf I.

doi:10.1016/s0143-4160(97)90062-2

Cited by 37 publications

(35 citation statements)

References 16 publications

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“…In agreement with our previous results, application of 5 µM ionomycin induced fusion of 3.5 ± 3.2% of CD63‐labeled lysosomes ( n = 197 cells; Figure 5a,d). Considering the strong increase of [Ca 2+ ] i by ionomycin which can reflect rather pathological conditions, we next applied “mild” electrogenic calcium ionophore ferutinin (Abramov & Duchen, 2003; Abramov, Zamaraeva, Hagelgans, Azimov, & Krasilnikov, 2001; Zamaraeva et al, 1997). Ferutinin (30 µM) induced fusion of ∼25.3 ± 3.6% of CD63‐expressing intracellular compartments ( n = 150 cells; Figure 5d).…”

Section: Resultsmentioning

confidence: 99%

Signal transduction in astrocytes: Localization and release of inorganic polyphosphate

Angelova

Iversen

Teschemacher

et al. 2018

Glia

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Inorganic polyphosphate (polyP) is present in every cell and is highly conserved from primeval times. In the mammalian cells, polyP plays multiple roles including control of cell bioenergetics and signal transduction. In the brain, polyP mediates signaling between astrocytes via activation of purinergic receptors, however, the mechanisms of polyP release remain unknown. Here we report identification of polyP‐containing vesicles in cortical astrocytes and the main triggers that evoke vesicular polyP release. In cultured astrocytes, polyP was localized predominantly within the intracellular vesicular compartments which express vesicular nucleotide transporter VNUT (putative ATP‐containing vesicles), but not within the compartments expressing vesicular glutamate transporter 2 (VGLUT2). The number of lysosomes which contain polyP was dependent on the conditions of astrocytes. Release of polyP from a proportion of lysosomes could be induced by calcium ionophores. In contrast, polyP release from the VNUT‐containing vesicles could be triggered by various physiological stimuli, such as pH changes, polyP induced polyP release and other stimuli which increase [Ca2+]i. These data suggest that astrocytes release polyP predominantly via exocytosis from the VNUT‐containing vesicles. © 2018 Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Signal transduction in astrocytes: Localization and release of inorganic polyphosphate

Angelova

Iversen

Teschemacher

et al. 2018

Glia

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“…The mechanisms involved in the ferutinin-induced NOS activation cannot be established from our present results, but they could be multifactorial. It has been reported that estrogen and membrane activity of aromatic and aliphatic derivatives of sesquiterpen alcohol carotane, isolated from Ferula genus, modulate hormone production via affecting the mobilization of intracellular calcium (Zamaraeva et al, 1997). Ferutinin may activate phospholipase C (PLC), which hydrolyzes phosphatidilinositol(4,5)biphosphate (PIP 2 ), to generate inositol(1,4,5)-trisphosphate (IP 3 ) and diacylglicerol, leading to the release of intracellular calcium stores and to DAG-dependent activation of protein kinase C (PKC) and activation of Ca 2+ channels with the consequent Ca 2+ influx (Berridge et al, 1983).…”

Section: Resultsmentioning

confidence: 99%

Ferutinin stimulates nitric oxide synthase activity in median eminence of the rat

Colman-Saizarbitoria

Boutros

Amesty

et al. 2006

Journal of Ethnopharmacology

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“…Endogenous non-channel ionophores are among the potential means of mediating and/or regulating mitochondrial ionic flux in vivo. 27,28 Ultimately, testing these hypotheses electrophysiologically will require new methodologies capable of detecting ionic currents at the sub-pA level.…”

Section: Potential Explanations For the Discrepancies Between Directlmentioning

confidence: 99%

Calculation of ion currents across the inner membrane of functionally intact mitochondria

Kane

Pavlov

2013

Channels

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M itochondrial ion transport systems play a central role in cell physiology. Rates of Ca2+ and K + transport across the inner mitochondrial membrane have been derived from the measurement of ion accumulation over time within functional isolated mitochondria or mitochondria of cultured cells. Alternatively, the electrical currents generated by ionic flux have been directly measured in purified and swollen mitochondrial samples (mitoplasts) or reconstituted channels, and typically range from 1 pA to several 100s pA. However, the direct electrophysiological approach necessarily requires extensive processing of the mitochondria prior to measurement, which can only be performed on isolated mitoplasts. To compare rates of mitochondrial ion transport measured in electrophysiological experiments to those measured in intact mitochondria and cells, we converted published rates of mitochondrial ion uptake into units of ionic current. We estimate that for monovalent ions, uptake by intact mitochondria at the rate of 1 nmolis equivalent to 0.2 fA of current per whole single mitochondrion (0.4 fA for divalent ions). In intact mitochondria, estimated rates of electrogenic cation uptake are limited to 1-100 fA of integral current per single mitochondrion. These estimates are orders of magnitude lower than the currents through mitochondrial channels directly measured via patch-clamp or artificial lipid bilayer approaches.

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Ionophoretic properties of ferutinin

Cited by 37 publications

References 16 publications

Signal transduction in astrocytes: Localization and release of inorganic polyphosphate

Signal transduction in astrocytes: Localization and release of inorganic polyphosphate

Ferutinin stimulates nitric oxide synthase activity in median eminence of the rat

Calculation of ion currents across the inner membrane of functionally intact mitochondria

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