Ionomics, transcriptomics and untargeted metabolomics analyses provide new insights into the Cd response and accumulation mechanisms of mulberry

Fan, Wei; Xia, Zhongqiang; Liu, Changying; Ma, Shuyu; Liu, Shuman; Wu, Yonghong; Zhu, Baozhong; Xu, Changzheng; Zhao, Aichun

doi:10.1016/j.envexpbot.2022.104821

Cited by 11 publications

(4 citation statements)

References 56 publications

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“…Extracts were analyzed by UPLC (Waters UPLC Acquity I‐Class PLUS) and QTOF system (Waters UPLC Xevo G2‐XS QTof) with a chromatographic column Acquity UPLC HSS T3, mobile phase A (0.1% formic acid aqueous solution), and mobile phase B (0.1% formic acid acetonitrile) (Fan et al, 2022). Metabolites need to be ionized before they can be detected by mass spectrometry.…”

Section: Methodsmentioning

confidence: 99%

Amelioration of saline‐alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis

Shao

Jia³

et al. 2023

Land Degrad Dev

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Soil salinization is a serious problem leading to ecological degradation. Melia azedarach is highly salt‐tolerant, and its application to saline‐alkali land is a promising strategy for restoring degraded lands. However, most studies only focus on the salt‐reducing effect of herbs on saline‐alkali land, and there are few studies on the metabolic adaptation mechanism of trees in the process of soil restoration in coastal saline‐alkali land. In this study, we analyzed the soil properties and metabolome of M. azedarach roots grown in low‐ (<3 g/kg; L), medium‐ (5 ~ 8 g/kg; M), and high‐ (>10 g/kg; H) salinity soils to explore the amelioration effect and adaptation mechanism of M. azedarach to soils with differential salinity. Cultivation of M. azedarach was associated with a decrease in the concentration of Na+ and increases in organic matter content and alkaline phosphatase and urease activities in the rhizosphere soil. The metabolome analysis revealed that a total of 382 (ESI+) and 277 (ESI−) differential metabolites (DEMs) were detected. The number of DEMs in roots rose with increased soil salinity, such as sugars and flavonoids in H versus L, and amino acids in M versus L. The most up‐regulated DEMs were 13‐S‐hydroxyoctadecadienoic acid, 2′‐Deoxyuridine, and 20‐hydroxyleukotriene B4. Combined analysis of soil properties and M. azedarach DEMs indicated that alkaline phosphatase activity was positively correlated with traumatic acid concentration. Taken together, these results indicate that M. azedarach has the potential to reduce soil salinity and enhance soil enzyme activity, and it can adapt to salt stress by regulating metabolites like sugars, amino acids, and flavonoids. This study provides a basis for understanding the mechanism underlying the adaptation of M. azedarach to saline‐alkali soil and its amelioration.

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Section: Methodsmentioning

confidence: 99%

Amelioration of saline‐alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis

Shao

Jia³

et al. 2023

Land Degrad Dev

View full text Add to dashboard Cite

show abstract

“…Extracts were analyzed by UPLC (Waters UPLC Acquity I-Class PLUS) and QTOF system (Waters UPLC Xevo G2-XS QTof) with a chromatographic column Acquity UPLC HSS T3, mobile phase A (0.1% formic acid aqueous solution), and mobile phase B (0.1% formic acid acetonitrile) (Fan et al, 2022). Metabolites need to be ionized before they can be detected by mass spectrometry.…”

Section: Uplc-q-tof-ms For Metabolite Analysismentioning

confidence: 99%

Amelioration of saline-alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis

Long

et al. 2023

Preprint

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Soil salinization is a serious problem leading to ecological degradation. Melia azedarach is highly salt-tolerant, and its application to saline-alkali land is a promising strategy for restoring degraded lands. In this study, we analyzed the soil properties and metabolome of M. azedarach roots grown in low- (< 3 g/kg; L), medium- (5~8 g/kg; M), and high- (> 10 g/kg; H) salinity soils to explore the amelioration effect and adaptation mechanism of M. azedarach to soils with differential salinity. Cultivation of M. azedarach was associated with a decrease in the concentration of Na and increases in organic matter content and alkaline phosphatase and urease activities in the rhizosphere soil. The metabolome analysis revealed that a total of 382 (ESI+) and 277 (ESI-) differential metabolites (DEMs) were detected. The number of DEMs in roots rose with increased soil salinity, such as sugars and flavonoids in H vs. L, and amino acids in M vs. L. The most up-regulated DEMs were 13-S-hydroxyoctadecadienoic acid, 2’-Deoxyuridine and 20-hydroxyleukotriene B4. Combined analysis of soil properties and M. azedarach DEMs indicated that alkaline phosphatase activity was positively correlated with traumatic acid concentration. Taken together, these results indicate that M. azedarach has the potential to reduce soil salinity and enhance soil enzyme activity, and it can adapt to salt stress by regulating metabolites like sugars, amino acids, and flavonoids . This study provided a basis for understanding the mechanism underlying the adaptation of M. azedarach to saline-alkali soil and its amelioration.

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“…Recently, the integrative analyses of metabolome and transcriptome have been widely used to screen out differentially accumulated metabolites and expressed genes in a high throughput manner, which efficiently prompts the exploration of potential mechanisms underlying main traits, such as flower coloration [22,[26][27][28], fruit quality [29][30][31][32] and stress resistance [33][34][35][36]. In Lagerstroemia indica, the different accumulation of malvidin-3,5,-di-O-glucoside was found to lead to its color diversity using metabolomic analysis, which could be addressed by the differential expression of F3 5 H and F3 H through further transcriptomic analysis [37].…”

Section: Introductionmentioning

confidence: 99%

Integrative Metabolome and Transcriptome Analyses Reveal the Molecular Mechanism of Yellow-Red Bicolor Formation in Kalanchoe blossfeldiana Petals

et al. 2023

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The winter pot kalanchoe (Kalanchoe blossfeldiana) is an ornamental plant with succulent leaves and clustered flowers in Crassulaceae, widely used as a potted flower or garden decoration. In nature, the bicolor petal is an interesting phenomenon, and breeders have succeeded in cultivating the winter pot kalanchoe with bicolored petals. However, its potential molecular mechanism of pigmentation is poorly understood. This study collected a yellow-red colored winter pot kalanchoe to investigate the molecular mechanism underlying its bicolor formation using the integrative analyses of metabolome and transcriptome. The metabolome results showed that both flavonoid and carotenoid co-existed in the winter pot kalanchoe petals, whereas only anthocyanin accumulation showed significant differences—about nineteen times higher in the red region than that in the yellow region. The differentially expressed genes were significantly enriched in the anthocyanin biosynthesis pathway, and the expression level of biosynthetic genes, including KbCHS, KbCHI, KbF3H, KbDFR, KbANS and KbGTs, were significantly upregulated in the red region. Moreover, transcription factors potentially regulating anthocyanin biosynthesis were predicted, and KbMYB2 and KbbHLH1 might play important roles in positively regulating anthocyanin biosynthesis in the red region. The findings reported here provide new insights into the understanding of petal bicolor formation mechanisms and will assist cultivar innovation in winter pot kalanchoe.

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Ionomics, transcriptomics and untargeted metabolomics analyses provide new insights into the Cd response and accumulation mechanisms of mulberry

Cited by 11 publications

References 56 publications

Amelioration of saline‐alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis

Amelioration of saline‐alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis

Amelioration of saline-alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis

Integrative Metabolome and Transcriptome Analyses Reveal the Molecular Mechanism of Yellow-Red Bicolor Formation in Kalanchoe blossfeldiana Petals

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