“…Whole body irradiation has been employed both to terminate and to generate tolerance. The involved doses, however, have been in the low to mid-lethal dose range (Nossal & Larkin, 1959;Anderson & Warner, 1976) and thus considerably greater than those employed herein.…”
mentioning
confidence: 87%
“…The basis of this difference among inactivating agents is not known but may relate to the marked alterations caused by irradiation to the surface topography of susceptible cells (Anderson & Warner, 1976).…”
“…Whole body irradiation has been employed both to terminate and to generate tolerance. The involved doses, however, have been in the low to mid-lethal dose range (Nossal & Larkin, 1959;Anderson & Warner, 1976) and thus considerably greater than those employed herein.…”
mentioning
confidence: 87%
“…The basis of this difference among inactivating agents is not known but may relate to the marked alterations caused by irradiation to the surface topography of susceptible cells (Anderson & Warner, 1976).…”
“…The radiobiology of lymphocytes has been extensively studied and distinct populations of lymphocytes have been identified by their differential sensitivity to ionizing radiation (1). Several reports indicate that B lymphocytes are more radiosensitive than T lymphocytes (2-5), but it is well recognized that resistance to ionizing radiation varies within subpopulations of both cell types (6)(7)(8)(9).…”
Phytohemagglutinin stimulated human lymphocytes exhibit a 20 fold increase in DNA repair synthesis.following ionizing radiation damage compared to the level of repair in unstimulated cells. The peak of repair synthesis coincides with that for DNA replication. Stimulated lymphocytes provide a relatively simple assay for ionizing radiation repair defects.
“…There are several reports (7)(8)(9)(10)(11) that some T cells are more radioresistant than B cells. We therefore studied whether B and T lymphoid stem cells could also be distinguished by their radiation sensitivities in vivo by competing the endogenous stem cells left immediately after irradiation with exogenous B and T genetically marked marrow.…”
T and B lymphocytes are descended from a common ancestor, a muhipotent stem cell (1, 2). The question arises as to the stage at which these two lineages diverge. Do multipotent stem cells directly generate B and T cells according to their local microenvironment, or do they first give rise to descendant stem cells that are restricted in their potentiality? There would be prima facie evidence for such committed stem cells, able to yield only one or other type of lymphocyte, if procedures were found that differentially replenish the mature B and T compartments of repopulated irradiated hosts. Abramson et al. (2) induced chromosome markers by irradiation and followed the progeny of uniquely marked individual stem cells. They found signs of a committed T stem cell in bone marrow. However, their protocol did not permit the characterization or isolation of committed stem cells. The formal objection has been raised (3) that the induction of the marker may itself have caused the apparent commitment. In the studies reported here, we have capitalized on naturally occurring B and T cell markers in the rat. We set out to confirm the existence of committed T stem cells by studying their surface antigenic properties and their radiosensitivity in vivo.In rats, a 95,000 mol we, heavily glycosylated glycoprotein defined by the mouse monoclonal antibody W3/13 (4, 5) is found on some bone marrow cells, on thymocytes, mature T lymphocytes, and immunoglobulin-secreting cells, but not B lymphocytes. This peculiar distribution prompted us to study this antigen on stem cells assayed by their ability to form T and B lymphocyte chimeras (6). Marrow from donors simultaneously carrying T and B alloantigenic genetic markers was incubated with W3/13 and sorted into bright or dim or dull fractions before injection into syngeneic irradiated hosts along with a fixed dose of competing host-type marrow. We found differential T and B lymphocyte chimerism in the recipients of W3/13 dim marrow.There are several reports (7-11) that some T cells are more radioresistant than B cells. We therefore studied whether B and T lymphoid stem cells could also be distinguished by their radiation sensitivities in vivo by competing the endogenous stem cells left immediately after irradiation with exogenous B and T genetically marked marrow. PVG rats were subjected to various doses of gamma irradiation and injected with a fixed dose of doubly marked marrow. We then looked for split chimerism in peripheral T and B lymphocytes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.