Ionic strength effect in HSA adsorption on mica determined by streaming potential measurements

Revealing deposition mechanism of colloid particles on human serum albumin monolayers

“…2 are well reflected by the electrokinetic model (depicted by solid lines) developed in Ref. [27] by considering a three dimensional charge distribution within the protein monolayer.…”

“…It should be mentioned that the commonly used mass coverage unit [27,38] is connected with the above thermodynamic surface concentration via the linear dependence a a…”

“…In Ref. [27] the adsorption of HSA on mica was studied by using the in situ streaming potential method. The influence of ionic strength and pH on the maximum coverage of irreversibly bound protein fraction was precisely evaluated.…”

KfrA plasmid protein monolayers on latex particles-electrokinetic measurements

“…This is an interesting estimate because knowing N c one can predict the bulk electrophoretic mobility of KfrA molecules, which is impractical using conventional methods (micro-electrophoretic measurements). The mobility can be calculated form the formula using the Lorenz-Stokes relationship previously applied for fibrinogen [ 25 ] and HSA [ 27 ] …”

“…This is so because the adsorbed KfrA molecules stick out from the shear plane, hence they become more exposed to the flow. In this case the 3D electrokinetic model should be more appropriate as previously demonstrated for human serum albumin adsorption [ 27 ]. According to this model the zeta potential of interfaces covered by protein molecules is given by the expression [ 23 ] …”