Ionic signalling mechanisms involved in neurokinin‐3 receptor‐mediated augmentation of fear‐potentiated startle response in the basolateral amygdala

Abstract

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Neuromedin B excites central lateral amygdala neurons and reduces cardiovascular output and fear‐potentiated startle

“…Surgical procedures and cannula placement were performed according to our previous publications (Boyle et al, 2022; Deng et al, 2009; Xiao et al, 2012, 2014) under aseptic conditions. Male (368 ± 10.4 g, n = 17) and female (225 ± 5.8 g, n = 20) SD rats were deeply anaesthetized using vaporized isoflurane and secured in a stereotaxic frame (Stoelting Co.).…”

“…ASR and FPS experiments were conducted using two identical SR‐LAB startle chambers with cylindrical animal holders (San Diego Instruments) as previously described (Boyle et al, 2022). White noise bursts (90 dB, 95 dB, and 105 dB WNBs) were elicited from a high‐frequency loudspeaker mounted 24 cm above each animal holder within each cabinet.…”

“…Surgical procedures and cannula placement were performed according to our previous publications (Boyle et al, 2022;Deng et al, 2009;Xiao et al, 2012Xiao et al, , 2014 & Watson, 2007). The end of the guide cannula was positioned 0.5-1 mm above the CeA.…”

Leptin excites basolateral amygdala principal neurons and reduces food intake by LepRb‐JAK2‐PI3K‐dependent depression of GIRK channels

“…Surgical procedures and cannulation were performed as described previously (Boyle et al, 2022). Male (349.9 ± 70.4 g, n = 45) and female (234.1 ± 35.9 g, n = 45) Sprague–Dawley (SD) rats at the age of 8–11 weeks were anaesthetized with 5% vaporized isoflurane until the loss of the righting response and placed in a stereotaxic frame (Stoelting Co).…”

“…Male (349.9 ± 70.4 g, n = 45) and female (234.1 ± 35.9 g, n = 45) Sprague–Dawley (SD) rats at the age of 8–11 weeks were anaesthetized with 5% vaporized isoflurane until the loss of the righting response and placed in a stereotaxic frame (Stoelting Co). Guide cannula (23 GA, 8.5 mm length; P1 Technologies Inc) were bilaterally implanted to target the BLA with coordinates from the rat brain atlas (from bregma, anteroposterior: −2.4 ~ −2.6 mm, mediolateral: ±4.9 ~ 5 mm, dorsoventral: −7.3 mm) (Boyle et al, 2022). The tip of the guide was positioned within or ~0.5 mm above the BLA.…”