Ionic liquids as adjuvants for the tailored extraction of biomolecules in aqueous biphasic systems

Pereira, Jorge Fernando Brandão; Lima, Álvaro Silva; Freire, Mara G.; Coutinho, João A. P.

doi:10.1039/c003578e

Cited by 170 publications

(154 citation statements)

References 41 publications

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“…different K 3 C 6 H 5 O 7 :C 6 H 8 O 7 mole fraction ratios) at ≈ 50 wt% were prepared and used for the determination of the binodal curves. The phase diagrams were determined through the cloud point titration method [32][33] at 25ºC and atmospheric pressure. The system compositions were determined by the weight quantification of all components added within ± 10 -4 g.…”

Section: Experimental Section Materialsmentioning

confidence: 99%

Single-step purification of ovalbumin from egg white using aqueous biphasic systems

Pereira

Cruz

Almeida

et al. 2016

Process Biochemistry

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The ability of aqueous biphasic systems (ABS) composed of polyethylene glycols of different molecular weights (PEG 400, 600 and 1000) and buffered aqueous solutions of potassium citrate/ citric acid (pH = 5.0 -8.0) to selectively extract ovalbumin from egg white was here investigated. Phase diagrams, tie-lines and tie-line lengths were determined at 25ºC and the partitioning of ovalbumin in these systems was then evaluated. Aiming at optimizing the selective extraction of ovalbumin in the studied ABS, factors such as pH, PEG molecular weight and amount of the phase-forming components were initially investigated with pure commercial ovalbumin. In almost all ABS, it was observed a preferential partitioning of ovalbumin to the polymer-rich phase, with extraction efficiencies higher than 90%. The best ABS were then applied in the purification of ovalbumin from the real egg white matrix. In order to ascertain on the ovalbumin purity and yield, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion high performance liquid chromatography (SE-HPLC) analyses were conducted, confirming that the isolation/purification of ovalbumin from egg white was completely achieved in a single-step with a recovery yield of 65%. The results obtained show that polymer-salt-based ABS allow the selective extraction of ovalbumin from egg white with a simpler approach and better performance than previously reported. Finally, it is shown that ovalbumin can be completely recovered from the PEG-rich phase by an induced precipitation using an inexpensive and sustainable separation platform which can be easily applied on an industrial scale.

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Section: Experimental Section Materialsmentioning

confidence: 99%

Single-step purification of ovalbumin from egg white using aqueous biphasic systems

Pereira

Cruz

Almeida

et al. 2016

Process Biochemistry

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“…Chen et al 37 isolated seven strains of the genus Bacillus (three strains of B. stearotheromophilus and B. licheniformis, and one of B. subtilis) and assessed the potential reaction of different carbon chain lengths. The substrates used were p-nitrophenol butyrate (C 4 ), p-nitrophenol caproate (C 6 ), p-nitrophenol caprylate (C 8 ), p-nitrophenol caprate (C 10 ), p-nitrophenol laurate (C 12 ), p-nitrophenol myristate (C 14 ), p-nitrophenol palmitate (C 16 ) and p-nitrophenol stearate (C 18 ). With the exception of one strain of B. licheniformis, which showed greater activity for medium carbon chains (C 10 ), the lipolytic activity of lipases was higher in short chain esters in the hydrolytic reaction.…”

Section: Esterification Potentialmentioning

confidence: 99%

“…After obtaining the fermented broth, purification strategies must be employed, such as reverse micellar systems, membrane processes, chromatography and aqueous two-phase systems (ATPS) with or without ionic liquids. [13][14][15][16] ATPS has been shown to be a powerful technique and offers many advantages as it is environmentally biocompatible, requires low interfacial tension, low energy, scales-up easily, and can operate continuously. The purification processes can change the structural and functional properties of the enzymes, rendering enzymatic characterization an important step in determining the process conditions and application potential.…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterisation of lipase from a new strain of Bacillus sp. ITP-001

et al. 2012

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Recebido em 26/9/11; aceito em 24/1/12; publicado na web em 15/5/12Lipases are characterised mainly by catalytic versatility and application in different industrial segments. The aim of this study was to biochemically characterise a lipase from a new strain of Bacillus sp. ITP-001. The isoelectric point and molecular mass were 3.12 and 54 kDa, respectively. The optima lipase activity was 276 U g -1 at pH 7.0 and a temperature of 80 °C, showing greater stability at pH 5.0 and 37 °C. , respectively determined by the Eadie-Scatchard method.

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“…This feature is a major benefit of ionic-liquid-based ABS given the difficulty of overcoming the limited polarity range of polymer-based ABS. 9 Polymer-polymer-based systems usually display two hydrophobic phases whereas polymer-salt ABS have both an extreme hydrophobic phase and a highly hydrophilic one. This limited difference in polarities between the coexisting phases prevents the widespread use of polymerbased ABS for extraction purposes since the tailoring and selective partitioning are difficult to achieve.…”

Section: Introductionmentioning

confidence: 99%

Aqueous biphasic systems: a benign route using cholinium-based ionic liquids

et al. 2013

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acIonic liquid based aqueous biphasic systems (ABS) have been the focus of a significant amount of research in the last decade. However, only (moderately) toxic and poorly biodegradable ionic liquids have been explored hitherto. Focusing on the development of more benign and sustainable approaches, a novel class of ABS using cholinium based ionic liquids is proposed. For the first time, it is shown that a large assortment of cholinium based ionic liquids is capable of undergoing liquid liquid demixing in the presence of aqueous solutions with strong salting out species. In order to assess the applicability of these systems for separation purposes, the partitioning of two antibiotics and/or their hydrochloride forms was also investigated. Cholinium based ABS are shown to be improved routes for the extraction of pharmaceuticals, achieving complete extractions in a single step by way of the proper tailoring of the phase forming components and their concentrations in the aqueous media.

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Ionic liquids as adjuvants for the tailored extraction of biomolecules in aqueous biphasic systems

Cited by 170 publications

References 41 publications

Single-step purification of ovalbumin from egg white using aqueous biphasic systems

Single-step purification of ovalbumin from egg white using aqueous biphasic systems

Biochemical characterisation of lipase from a new strain of Bacillus sp. ITP-001

Aqueous biphasic systems: a benign route using cholinium-based ionic liquids

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