Ionic liquid synergistic effect between preparation of hybrid supports and immobilization of lipase applied to esters production

Martins, Sílvia; Andrade, Suellen M. S.; Fricks, Alini Tinoco; Lima, Álvaro Silva; Silva, Daniel P.; Figueiredo, Renan Tavares; Freitas, Lisiane Santos; Padilla, Rebeca Yndira Cabrera; Soares, Cleide Mara Faria; Souza, Ranyere Lucena de

doi:10.1007/s10973-020-10408-4

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…For a deeper understanding of enzymatic efficiency, the FTIR spectrum's amide I region (1600–1700 cm −1 ) was deconvoluted. This region is sensitive to changes in the secondary structure, which consists of specific elements: β sheet (1610–1640 cm −1 ), random structure (1640–1650 cm −1 ), α ‐helix (1650–1658 cm −1 ), and β ‐turn (1660–1700 cm −1 ) 50,51 . The α ‐helix corresponds to the mobile arrangement of the enzyme that allows access to the active site; the greater its content, the more restricted the access to the active site.…”

Section: Resultsmentioning

confidence: 99%

“…This region is sensitive to changes in the secondary structure, which consists of specific elements: ⊎ sheet (1610-1640 cm −1 ), random structure (1640-1650 cm −1 ), ⊍-helix (1650-1658 cm −1 ), and ⊎-turn (1660-1700 cm −1 ). 50,51 The ⊍-helix corresponds to the mobile arrangement of the enzyme that allows access to the active site; the greater its content, the more restricted the access to the active site. The decrease in the ⊍-helix content constitutes a greater opening for the substrate to access the enzymatic active site, favoring enzymatic performance.…”

Section: Ftir Analysismentioning

confidence: 99%

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Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Santana,

Barros,

Canuto

et al. 2023

J Sci Food Agric

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BackgroundThis study explores the use of liquid‐liquid extraction (LLE) with thermosensitive polymers for producing laccase (Lac) from Pleurotus sajor‐caju. This process leverages liquid waste from the citrus industry, specifically pulp wash. The research delves into extractive fermentation and thermoseparation, both processes were facilitated by a polymer exhibiting a lower critical solution temperature (LCST) transition.ResultsKey factors considered include the choice of polymer, its concentration, pH, separation temperature, and the behavior of the polymer‐rich phase post‐extractive fermentation concerning LCST. Notably, under conditions of 45% by weight of Pluronic L‐61 and a pH of 5.0 at 25°C, the laccase was achieved an enhancement in the purification factor (PF) of 28.4‐fold, compared to the laccase obtained directly from the fermentation process on the 8th day. There was an 83.6% recovery of the laccase enzyme in the bottom phase of the system. Additionally, the unique properties of Pluronic L‐61, which can induce phase separation and also allow for thermoseparation, led to a secondary fraction (aqueous solution) of laccase with PF of 2.1 ± 0.1‐fold (at 32 ± 0.9 °C and 30 ± 0.3 min without stirring) from polymeric phase (top‐phase). The FTIR analysis validated the separation data, particularly highlighting the α‐helix content in the Amide I region (1600 ‐ 1700 cm‐1).ConclusionIn summary, the insights from this study pave the way for broader industrial applications of these techniques, underscoring benefits like streamlined process integration, heightened selectivity, and superior separation efficacy.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 99%

Section: Ftir Analysismentioning

confidence: 99%

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Santana,

Barros,

Canuto

et al. 2023

J Sci Food Agric

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“…This is conductive to the mass transfer and reduce reaction time. But excess temperature directly leads to the deactivation of lipase . Adak et al reported that higher residual activity of lipase was observed in [C 16 MIM]Br environment compared to lipase alone, indicating that the presence of IL improve the thermal stability of lipase.…”

Section: Biofuel Productionmentioning

confidence: 99%

Energy Applications of Ionic Liquids: Recent Developments and Future Prospects

Zhou,

Gui,

Sun

et al. 2023

Chem. Rev.

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Ionic liquids (ILs) consisting entirely of ions exhibit many fascinating and tunable properties, making them promising functional materials for a large number of energy-related applications. For example, ILs have been employed as electrolytes for electrochemical energy storage and conversion, as heat transfer fluids and phase-change materials for thermal energy transfer and storage, as solvents and/or catalysts for CO2 capture, CO2 conversion, biomass treatment and biofuel extraction, and as high-energy propellants for aerospace applications. This paper provides an extensive overview on the various energy applications of ILs and offers some thinking and viewpoints on the current challenges and emerging opportunities in each area. The basic fundamentals (structures and properties) of ILs are first introduced. Then, motivations and successful applications of ILs in the energy field are concisely outlined. Later, a detailed review of recent representative works in each area is provided. For each application, the role of ILs and their associated benefits are elaborated. Research trends and insights into the selection of ILs to achieve improved performance are analyzed as well. Challenges and future opportunities are pointed out before the paper is concluded.

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Physical adsorption used to the immobilization of Burkholderia cepacia lipase into powder polymeric supports

Corrêa

Bortolozo

Osório

et al. 2021

J Therm Anal Calorim

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Ionic liquid synergistic effect between preparation of hybrid supports and immobilization of lipase applied to esters production

Cited by 6 publications

References 55 publications

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Energy Applications of Ionic Liquids: Recent Developments and Future Prospects

Physical adsorption used to the immobilization of Burkholderia cepacia lipase into powder polymeric supports

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