Ionic Components of Wound Current at Mouse Skin Incisional Wounds

Abstract

It has been assumed that skin wound currents are produced by passive ion leakage from wounded skin. However, what ions contribute to the skin wound current? Thirty male C57BL/6 mice were used in this study. A lancet wound (about 5mm in length) was made by cutting into the full thickness skin. We measured the dynamic time courses of individual ion flux with ion-selective probes at skin wound. The probe is aligned perpendicular to the surface of skin wound. All ions , Ca 2+ and H + contribute to the wound currents at skin wounds. A most significant observation is that the skin wound currents are carried mainly by the largest sodium and chloride efflux, then potassium, while the calcium and hydrogen are the least.

Effect of electroacupuncture Bangci on chloride flux at mouse skin incisional wounds

“…Our previous study confirmed that ions flux (Na + , Cl -, K + , Ca 2+ , H + ) contributes to mouse skin wound currents (1). In addition, we found that major ions flux of the wound current involved Na + and Cl -.…”

“…Several previous studies have detec- ted wound current in various tissues in a variety of animals, including human skin (11)(12) and rodent cornea and skin (13)(14)(15)(16). In our previous study, we found that Clflux was the main ionic flux and major contributor to the skin wound current (1). The results of our study demonstrated that Clflux increased significantly after mouse skin was incised compared to that in normal skin.…”

“…Our previous study showed that Clflux strongly contributes to wound current in mice (1). A previous study showed that enhancing or decreasing Cl − flux with clinically approved pharmacological agents increased or decreased endogenous wound electric currents, respectively.…”

“…Wound current plays a very important role in the process of wound healing (1)(2). Exogenous fields are thought to have positive effects on wound current to accelerate wound healing (3)(4).…”

Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes

“…Ideally, any biological system of interest should be studied with the minimum interference and under the most physiological condition possible. Such criteria are attained by the non-invasive ion-selective vibrating probe, which has been used for measuring multiple transmembrane ion fluxes in a wide variety of experimental systems, including Drosophila (Browne and O'Donnell, 2016), zebrafish (Guh et al, 2016), mouse skin (Sun et al, 2015), roots (He et al, 2015), Daphnia (Stensberg et al, 2014), C. elegans (Adlimoghaddam et al, 2014), etc. In pollen tubes, quantitative measurements of extracellular ion fluxes using the ion-selective vibrating probe have been fundamental in establishing the role of major ions (especially Ca 2+ , H + , K + , and anions) in apical growth.…”