Ion transport, membrane fluidity and haemoglobin conformation in erythrocyte from patients with cardiovascular diseases: Role of augmented plasma cholesterol

Effects of weight loss on erythrocyte membrane composition and fluidity in overweight and moderately obese women

“…First, improved membrane fluidity has been shown to increase the rate of oxygen exchange between erythrocytes and tissue [31] and, consequently, oxygen-dependent aerobic metabolism and thermogenesis, thus, facilitating the greater weight loss shown in Group II. Second, increased membrane fluidity has been shown to improve membrane-bound insulin receptor sensitivity and, consequently, to reduce both peripheral insulin resistance and the plasma levels of insulin as shown by the lower HOMA values found in Group II.…”

Chronic Kidney Disease Predicts Impaired Membrane Microviscosity of Red Blood Cells in Hypertensive and Normotensive Subjects

“…2T' ‖ : outer hyperfine splitting, 2T' ⊥ : inner hyperfine splitting, T: tesla The order parameter (S) was calculated from 2T' ‖ and 2T' ⊥ values according to the formula (13). S= (a n /a' n ) (T' ‖ -T' ⊥ ) /(Tzz-Txx) a n /a' n : isotropic coupling constant, Tzz and Txx: hyperfine constants The greater the value of the order parameter (S), the lower the membrane fluidity of RBCs (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). Relationships between membrane fluidity of red blood cells and estimated glomerular filtration rate, plasma 8-iso-prostaglandin F2α, or plasma nitric oxide-metabolite levels in hypertensive and normotensive subjects: The order parameter (S) of RBCs was inversely correlated with eGFR value (r = -0.309, n = 105, P < 0.01) ( Figure 5), a finding that indicated the reduced membrane fluidity of RBCs was associated with decreased kidney function.…”

“…This finding might be consistent with previous findings showing that the cell membranes were stiffer and less fluid in hypertension. [13][14][15][16][17][18][19][20][21][22][23][24][25] Furthermore, it was found that the value of eGFR was inversely correlated with the order parameter (S) of RBCs, indicating that reduced membrane fluidity of RBCs might be associated with decreased kidney function. In the separate analysis of men and women, the present study also demonstrated a significant association between reduced membrane fluidity of RBCs and lower eGFR value.…”

“…10,13) In addition, 5-nitroxide stearate could reveal the properties of superficial membrane layers, and data regarding the alterations in membrane fluidity in hypertension by using 5-nitroxide stearate have been reported. [13][14][15][16][17][18][19][20][21][22][23][24][25] After incubation with 5-nitroxide stearate, the ESR measurements were immediately performed using an ESR spectrometer (Model Jeol JES-FE2XG, Nihon Denshi, Tokyo) with a microwave unit (Model Jeol ES-SCXA, Nihon Denshi). The microwave power was 5 mW and the modulation frequency was 100 kHz with a modulation amplitude of 0.2 mT (tesla).…”

Promoter cloning and characterization of the rabbit BK channel β1 subunit gene

“…However, the precise mechanisms for HC induced SOD keep unclear. HC has been demonstrated to cause changes in membrane cholesterol content and membrane structure of sorts of cells including SO cell (Bhandaru et al, 1982;Armstrong et al, 1986;Chen et al, 2003;Luneva et al, 2007). Data from experimental animals showed that the changed membrane cholesterol content may directly affect the normal functions of BK channel in smooth muscles (Crowley et al 2003;Lam et al, 2004;Shmygol et al, 2007).…”