Ion-pair reversed phase liquid chromatography-indirect conductivity detection and response deviations of underivatised amino acids

Analysis of stable and radioactive isotopes from bone collagen provides useful information to archaeologists about the origin and age of bone artifacts. Isolation and analysis of single amino acids from the proteins can provide additional and more accurate information by removing contamination and separating a bulk isotope signal into its constituent parts. In this paper, we report a new method for the separation and isolation of underivatized amino acids from bone collagen, and their analysis by isotope ratio MS and accelerator MS. RP chromatography is used to separate the amino acids with nonpolar side chains, followed by an ion pair separation to isolate the remaining amino acids. The method produces single amino acids with little or no contamination from the separation process and allows for the measurement of accurate stable isotope ratios and pure samples for radiocarbon dating.

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The simultaneous determination of 1‐aminocyclopropane‐1‐carboxylic acid and cyclopropane‐1,1‐dicarboxylic acid in Lycopersicum esculentum by high‐performance liquid chromatography—electrospray tandem mass spectrometry

Pétritis¹,

Koukaki²,

Koussissi³

et al. 2003

Phytochemical Analysis

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Varying concentrations of cyclopropane-1,1-dicarboxylic acid (CDA), an inhibitor of 1-aminocyclopropane-1-carboxylic acid oxidase, added to the solid culture medium of tomato nodal shoot segments resulted in a reduction in the level of endogenous ethylene according to the concentration of inhibitor applied. Following treatment with inhibitor, plants were homogenised and the concentrations of CDA and of 1-aminocyclopropane-1-carboxylic acid (ACC) were measured simultaneously in the resulting juice using an HPLC-ESI/MS-MS method. The levels of CDA and ACC measured in the plant tissues were associated with the concentration of inhibitor added to the solid medium. The HPLC-ESI/MS-MS method described produced limits of detection of 0.8 pmol for ACC and of 4 pmol for CDA.

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Ion-pair reversed phase liquid chromatography-indirect conductivity detection and response deviations of underivatised amino acids

Cited by 10 publications

References 40 publications

Evaporative light scattering detection for in-line monitoring of stopped-flow liquid chromatography-nuclear magnetic resonance analysis of compounds with weak or no chromophore groups

Evaporative light scattering detection for in-line monitoring of stopped-flow liquid chromatography-nuclear magnetic resonance analysis of compounds with weak or no chromophore groups

Preparative separation of underivatized amino acids for compound‐specific stable isotope analysis and radiocarbon dating of hydrolyzed bone collagen

The simultaneous determination of 1‐aminocyclopropane‐1‐carboxylic acid and cyclopropane‐1,1‐dicarboxylic acid in Lycopersicum esculentum by high‐performance liquid chromatography—electrospray tandem mass spectrometry

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