The simultaneous determination of 1‐aminocyclopropane‐1‐carboxylic acid and cyclopropane‐1,1‐dicarboxylic acid in Lycopersicum esculentum by high‐performance liquid chromatography—electrospray tandem mass spectrometry

Abstract

Varying concentrations of cyclopropane-1,1-dicarboxylic acid (CDA), an inhibitor of 1-aminocyclopropane-1-carboxylic acid oxidase, added to the solid culture medium of tomato nodal shoot segments resulted in a reduction in the level of endogenous ethylene according to the concentration of inhibitor applied. Following treatment with inhibitor, plants were homogenised and the concentrations of CDA and of 1-aminocyclopropane-1-carboxylic acid (ACC) were measured simultaneously in the resulting juice using an HPLC-ESI/MS-MS method. The levels of CDA and ACC measured in the plant tissues were associated with the concentration of inhibitor added to the solid medium. The HPLC-ESI/MS-MS method described produced limits of detection of 0.8 pmol for ACC and of 4 pmol for CDA.

The dependence of leaf senescence on the balance between 1‐aminocyclopropane‐1‐carboxylate acid synthase 1 (ACS1)‐catalysed ACC generation and nitric oxide‐associated 1 (NOS1)‐dependent NO accumulation in Arabidopsis

“…Acetyl‐CoA carboxylase was extracted from 200 mg tissue samples as described by Petritis et al . (, ) with minor modifications. Supernatants were sequentially passed through a column containing C 18 adsorbent (Oasis MCX, 30 μm, 3 cc/60 mg; Waters, Milford, MA, USA), then the column was eluted using 1 ml of 1 mol·l −1 ammonia water (chromatographic methanol as solvent).…”

Protocol: An updated integrated methodology for analysis of metabolites and enzyme activities of ethylene biosynthesis

“…In 1979, Lizada and Yang published a method to quantify ACC indirectly by liberating ethylene from ACC with NaOCl in the presence of Hg 2+ [ 5 ]. Since then, several methods have been published that allow the direct quantification of ACC by gas chromatography-mass spectrometry (GC-MS) [ 6 ], liquid chromatography-mass spectrometry (LC-MS) [ 7 ] or capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) [ 8 ]. Each technique has its own advantages and disadvantages.…”

Quo vadis plant hormone analysis?

“…carbon molecular sieve) and is then released into a smaller volume by heating the adsorbent. In addition to GC and GC-MS (Smets et al 2003) approaches, photo acoustic laser spectrophotometry (PALS; Cristescu et al 2008), LC-MS (Petritis et al 2003) and Ce-LIF (Liu et al 2004) methods for ethylene (or ACC) determination have been published. PALS offers higher detection sensitivity (ppt level) than GC and is highly selective for particular substances.…”