Ion-pair high-performance liquid chromatographic assay method for the assessment of clarithromycin stability in aqueous solution and in gastric juice

Sensitive determination of clarithromycin in human plasma by high-performance liquid chromatography with spectrophotometric detection

“…Although in comparison with other macrolides, clarithromycin has somehow better acid stability, back-extraction of clarithromycin into acid has not been tried by previous investigators. In fact, clarithromycin is rapidly degradated in 0.1% hydrochloric acid [15], which could lead to a incorrect conclusion that back-extraction of clarithromycin into acid is not possible.…”

“…HPLC-UV method has been reported for plasma determination of other related macrolides such as erythromycin [12], roxithromycin [13] and oleandomycin [14] with limit of quantification (LOQ) of 0.25 g/ml. In our knowledge, there is only one published HPLC-UV method for clarithromycin assay in gastric juice with LOQ of 1.56 g/ml [15]. However, much higher sensitive HPLC-UV method is required for plasma clarithromycin assay, which has a maximum plasma concentration of 0.78 ± 0.25 g/ml following an oral dose of 250 mg [8].…”

Development and Validation of RP-HPLC Method for Quantitation of Clarithromycin in Matrix Tablet Dosage Form

“…Literature survey revealed that various methods have been developed for the estimation of clarithromycin from laboratory prepared mixtures, pharmaceutical preparations and biological matrices (such as human plasma) through automated solid phase extraction and electrochemical detection, liquid chromatographic electrospray tandem mass spectrometry and high performance liquid chromatography. [4][5][6][7] But most of these reported methods were cumbersome due to poor resolution, troublesome mobile phase (buffers) preparation and lengthy run time, hence difficult for routine analysis. Therefore, the aim of the present work was to establish a rapid, simple, precise, specific, accurate, and cost-effective RP-HPLC method according to ICH guidelines Q2 (R1) for routine analysis of clarithromycin in pharmaceutical formulations.…”

Development and Validation of an HPLC Method for the Determination of the macrolide antibiotic Clarithromycin using Evaporative Light Scattering Detector in raw materials and Pharmaceutical Formulations

“…These methods are based either on the derivatization of the molecule before the chromatographic column due to its low absorptivity or on the ion-pair formation followed by electrochemical detection with amberometric or coulometric detection [9][10][11][12][13][14][15] . There also references using spectrochemical methods 16,17 , gas chromatography, microbiological methods, thin layer chromatography (TLC), mass spectrometry 18,19 , capillary electrophoresis 20 , HPLC 21,22 and electrophoresis 23 .…”