Ion-pair chromatography on a porous graphitic carbon stationary phase for the analysis of twenty underivatized protein amino acids

Chaimbault, Patrick; Pétritis, Konstantinos; Elfakir, Claire; Dreux, M.

doi:10.1016/s0021-9673(99)00863-8

Cited by 116 publications

(62 citation statements)

References 31 publications

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“…We modified the analytical method for the quantification of underivatized amino acids 10 to quantify the tripeptides. The hydrolysates were dissolved at 5 mg/ml in distilled water.…”

Section: Dpp-iv Inhibition Assaymentioning

confidence: 99%

Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity

Hatanaka

Kawakami

Uraji

2014

Journal of Enzyme Inhibition and Medicinal Chemistry

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The collagen tripeptide fragments Gly-Ala-Hyp, Gly-Pro-Ala and Gly-Pro-Hyp were generated by hydrolyzing collagen from pig-skin, cattle-skin, fish-scales and chicken-feet, respectively, with Streptomyces collagenase. Collagenase treatment increased the concentration of tripeptides in the hydrolysates by 13-15% (w/w). Of the three peptides, Gly-Pro-Hyp was a true peptidic inhibitor of dipeptidylpeptidase-IV (DPP-IV), because DPP-IV could not hydrolyze the bond between Pro-Hyp. This tripeptide was a moderately competitive inhibitor (K i ¼ 4.5 mM) of DPP-IV, and its level in the collagen hydrolysates could be greatly increased (4-9% [w/w]) using Streptomyces collagenase.

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“…We modified the analytical method for the quantification of underivatized amino acids 10 to quantify the tripeptides. The hydrolysates were dissolved at 5 mg/ml in distilled water.…”

Section: Dpp-iv Inhibition Assaymentioning

confidence: 99%

Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity

Hatanaka

Kawakami

Uraji

2014

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…These results may be related to several limita" ons that were observed in those studies, such as the limited number of itera" ons for each of the deriva" za" on condi" ons [22][23][24], using either peak height or peak area without evalua" ng the calibra" on linearity, sensi" vity, or the limit of detec" on (LOD), using either SCAN mode or SIM mode, or using rela" vely long GC temperature programs (e.g. 54 min [25], 62 min [26], and 90 min [27]). …”

Section: Resultsmentioning

confidence: 99%

Method for determination of amino acid content in protein products for medical use

Macovescu¹,

Chelaru²,

Gurau³

2017

LFJ

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ABSTRACT. The paper presents a method for determina$ on of amino acid content in collagen biomaterials for medical use produced in the Collagen Department of INCDTP -Division ICPI and its valida$ on. The method has three stages: the fi rst consists in hydrolysing the collagen biomaterial sample down to amino acids; the second step refers to deriva$ za$ on of amino acids with N, O-bis (trimethylsilyl) trifl uoroacetamide (BSTFA) which is a silyla$ on reagent; deriva$ zed amino acids are detected using a mass spectrometer a+ er gas chromatography. The method was validated to establish performance parameters and to check compliance with the intended purpose. KEY WORDS: collagen, amino acids, biomaterials METODĂ PENTRU DETERMINAREA CONŢINUTULUI DE AMINOACIZI DIN PRODUSELE PROTEICE PENTRU UZ MEDICALREZUMAT. Se prezintă o metodă de determinare a conţinutului de aminoacizi din biomaterialele colagenice pentru uz medical produse în Departamentul Colagen al INCDTP -Sucursala ICPI şi validarea ei. Metoda are trei etape: prima constă în hidroliza probei de biomaterial colagenic până la stadiul de aminoacizi; a doua etapă se referă la deriva$ zarea aminoacizilor cu N, O-bis (trime$ lsilil) trifl uoracetamidă (BSTFA) care este un reac$ v de sililare; aminoacizii deriva$ zaţi sunt detectaţi pe un spectrometru de masă după gaz cromatografi e. Metoda a fost validată pentru a se stabili parametrii de performanţă şi pentru verifi carea conformării cu scopul propus. CUVINTE CHEIE: colagen, aminoacizi, biomateriale PROCÉDÉ DE DÉTERMINATION DE LA TENEUR EN ACIDES AMINÉS DANS DES PRODUITS PROTÉIQUES À USAGE MÉDICALRÉSUMÉ. On présente une méthode de détermina$ on de la teneur en acides aminés de biomatériaux de collagène à usage médical fabriqués dans le Département Collagène de INCDTP-ICPI et sa valida$ on. La méthode comporte trois étapes : la première consiste en l'hydrolyse de l'échan$ llon de biomatériau de collagène au stade des acides aminés ; la deuxième étape se rapporte à la dériva$ sa$ on d'acides aminés avec du N, O-bis (triméthylsilyl) trifl uoroacétamide (BSTFA) qui est un réac$ f de silyla$ on ; les acides aminés dérivés sont détectés sur un spectromètre de masse après chromatographie en phase gazeuse. La méthode a été validée pour établir les paramètres de performance et vérifi er la conformité avec l'objec$ f prévu.

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“…Porous graphite carbon (PGC) is the best adsorbent for the separation, in brief, Hypercarb TM has been widely applied for the separation of aromatic compounds, racemic mixture, and polymers [42][43][44][45][46]. Then, we finally demonstrated the separation of aromatic compounds with the C70 column comparted to the separation by the PGC column.…”

Section: Separation Of the Aromatic Compounds With C70 Column And Pormentioning

confidence: 99%

Development of a C<sub>70</sub>-Fullerene Bonded Silica-Monolithic Capillary and Its Retention Characteristics in Liquid Chromatography

et al. 2017

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In this report, C 70 -fullerene (C70), which has aspheric structure and greater π electrons compared to C 60 -fullerene (C60), was effectively immobilized onto the surface of a silica-monolithic capillary, and then the retention characteristics for polycyclic aromatic hydrocarbons (PAHs) due to π-π interaction were evaluated in liquid chromatography (LC). For the immobilization of C70, a C70 derivative via thermal reactive agent, perfluorophenylazide (PFPA) and N-hydroxysuccinimide (NHS) was synthesized. In comparison with a typical thermal reaction in an oil bath and a microwave, the latter reaction provided higher yield. Then, both the C70 and C60 monoliths were evaluated by LC with a variety of PAHs as the solutes. The results suggested that the specific interactions, such as dipole and induced dipole interactions were contributed between the bonded C70 and PAHs. Absorption spectra of PAHs with C60 or C70 and a computer simulation also supported these interactions. Furthermore, the comparison of the separations for PAHs and brominated benzenes with the C70 column and a porous graphite carbon packed column suggested that the interactions based on the polarization of the molecules contributed the separations.

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Ion-pair chromatography on a porous graphitic carbon stationary phase for the analysis of twenty underivatized protein amino acids

Cited by 116 publications

References 31 publications

Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity

Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity

Method for determination of amino acid content in protein products for medical use

Development of a C<sub>70</sub>-Fullerene Bonded Silica-Monolithic Capillary and Its Retention Characteristics in Liquid Chromatography

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