Development of a C₇₀-Fullerene Bonded Silica-Monolithic Capillary and Its Retention Characteristics in Liquid Chromatography

Abstract

In this report, C 70 -fullerene (C70), which has aspheric structure and greater π electrons compared to C 60 -fullerene (C60), was effectively immobilized onto the surface of a silica-monolithic capillary, and then the retention characteristics for polycyclic aromatic hydrocarbons (PAHs) due to π-π interaction were evaluated in liquid chromatography (LC). For the immobilization of C70, a C70 derivative via thermal reactive agent, perfluorophenylazide (PFPA) and N-hydroxysuccinimide (NHS) was synthesized. In comparison with a typical thermal reaction in an oil bath and a microwave, the latter reaction provided higher yield. Then, both the C70 and C60 monoliths were evaluated by LC with a variety of PAHs as the solutes. The results suggested that the specific interactions, such as dipole and induced dipole interactions were contributed between the bonded C70 and PAHs. Absorption spectra of PAHs with C60 or C70 and a computer simulation also supported these interactions. Furthermore, the comparison of the separations for PAHs and brominated benzenes with the C70 column and a porous graphite carbon packed column suggested that the interactions based on the polarization of the molecules contributed the separations.

Retention Behavior of Polycyclic Aromatic Compounds in a Novel Polymer-Based Stationary Phase Liquid Chromatography

“…Development of novel stationary phases has been regarded as a key approach to create more efficient and cost-effective separations in chromatography. In fact, various types of new stationary phases have been developed in liquid chromatography (LC) [1][2][3][4][5][6][7][8][9][10], however there is a still long way to get the final systematic interpretation of the whole LC retention mechanism.…”

Molecular Shape Selectivity for Polycyclic Aromatic Compoundson a Poly(4-vinylpyridine) Stationary Phase in Liquid Chromatography

“…Octadecylsilica (ODS) is widely employed as a stationary phase in LC because of the several advantageous features such as a good versatility, high separation performance, and significantly wide commercial availability with a reasonable cost [11][12][13]. However, there are still many types of complex mixtures, consisted of many closely-related components, that are quite difficult to be separated completely by single separation on typical ODS phases [14][15][16][17][18][19][20][21].…”

Separation of halogenated benzenes enabled by investigation of halogen–π interactions with carbon materials

“…We also uncovered the possibility for the separation of halogenated aromatic compounds resulting from the efficient halogen-p interactions in HPLC. 44 The aromatic halogens, such as polychlorinated biphenyls (PCBs) 45,46 and polybrominated diphenyl ethers (PBDEs), [47][48][49] are widely known to be highly toxic; therefore, we need monitor the concentration of these pollutions in our environment. Usually, these aromatic halogens are analyzed by gas-chromatography or reverse-phase liquid chromatography, which involve complicated preparation processes.…”