Ion-beam-induced deoxyribose nucleic acid transfer

Anuntalabhochai, S.; Chandej, R.; Phanchaisri, B.; Lei, Yu; Vilaithong, T.; Brown, Ian

doi:10.1063/1.1362338

Cited by 44 publications

(17 citation statements)

References 13 publications

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“…3 In ion-beam-induced DNA transfer only the cell envelope is bombarded in order to allow a subsequent transfer of whole DNA into the internal cell region. 7 A recent report 10 described the interaction of energetic ions with bacterial cells, inducing direct DNA transfer into E. coli, indicating that ion beams with an energy such that the ion range is , but none of them has been well supported experimentally.…”

Section: −10mentioning

confidence: 99%

Untitled

Apavatjrut¹,

Alisi²,

Phanchaisri³

et al. 2003

ScienceAsia

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Although the technology of ion-beam-induced gene transfer into either plant or bacterial cells has been successfully established, relevant mechanisms have not been understood. This work aimed to study the process of induction and thus to develop applications of ion beam bioengineering. Cells of various plant tissues were bombarded in vacuum with argon and nitrogen ion beams at energies of 15-30 keV with fluences ranging from 5 × 10 14 -3 × 10 16 ions/cm 2 . The ion bombardment effects on tissue viability and neutral red dye molecule transfer into the cells through the cell envelope were investigated. The results showed that the characteristics of the tissue survival from the ion bombardment and penetration of the dye molecules into the cells through the cell envelope depended on ion species, energy and fluence. For 30-keV argon-ion bombardment at a fluence of 2 × 10 15 ions/cm 2 , the dye molecules entered the cells without fatal injury, whereas under other conditions, the dye either did not enter the cells or stained the nuclei. On the cell envelope surface, ion-bombardment-induced crater-like structures were observed. Calculations indicated that exogenous molecule transfer into living plant cells can be achieved by ion beams with appropriate physical parameters such that the ion range and the radiation damage range lie within the solid cell wall thickness.

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Section: −10mentioning

confidence: 99%

Untitled

Apavatjrut¹,

Alisi²,

Phanchaisri³

et al. 2003

ScienceAsia

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“…There is not only energy absorption (just like c-ray and X-ray), but also mass deposition, and charge exchange (Yu 1998;Wilaithong et al 2000;Anuntalabhochai et al 2001). The survival ratio of low energy ion beam implantation in a recent study was oscillations decreasing with the dose, which did not follow the exponential law.…”

Section: Comparisons Of N218 and P602 Mutantmentioning

confidence: 95%

Mutant of a Xylanase-producing Strain of Aspergillus niger in Solid State Fermentation by Low Energy Ion Implantation

Yao

et al. 2005

World J Microbiol Biotechnol

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In order to obtain an industrial strain with higher xylanase production, the original strain of Aspergillus niger was mutated by N + implantation (10 keV, 1.04 · 10 15 -2.08 · 10 15 ions/cm 2 ), and a mutant P602 was obtained. The mutant showed xylanase production increased from 2989 to 6320 IU/g and the fermentation time was decreased from 72 h to 64 h. The fermentation temperature range was 28-32°C which was a little wider than the original strain.

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“…The post biological treatment for DNA transfer is carried out immediately after ion beam bombardment normally in standard biological procedures. Using the critical ion beam conditions, we succeeded in DNA transfer in bacterial cells of Escherichia coli (E. coli) [6,7]. Fig.…”

Section: Ion Beam Induction Of Gene Transfermentioning

confidence: 99%

“…Two varieties of local rice (Oryza sativa indica), purple glutinous rice (Kum Doi Saket) and Thai jasmine rice (KDML 105), were chosen for the mutation induction purpose [15,16]. Thousands of seeds of the rice were implanted with nitrogen ions at energy of 30 -60 keV to fluences of an order of 10 16 ions/cm 2 and mutants were selected from grown plants of the seeds.…”

Section: Ion Beam Induction Of Mutationsmentioning

confidence: 99%

Low-energy Ion Beam Biology Research at Chiang Mai University

Anuntalabhochai

2011

Trans. Mat. Res. Soc. Japan

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$ Both authors contributed equallyIon beam at energy lower than tens of keV has been found to have significant biological effects on living materials. The finding has led to applications of ion-beam induced mutation and gene transfer. From the theoretical point of view, the low-energy ion beam effects on biology are difficult to understand with the knowledge of high-energy ion irradiation of biological matter since the ion range is so short that the ions can hardly directly interact with the key biological molecules for the changes. This paper introduces interesting aspects of low-energy ion beam biology, including basis of ion beam biotechnology and recent developments achieved in Chiang Mai University in relevant applications such as mutation and gene transfer and investigation on mechanisms involved in the low-energy ion interaction with biological matter such as eV-keV ion beam bombardments of naked DNA and the cell envelopes.

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Ion-beam-induced deoxyribose nucleic acid transfer

Cited by 44 publications

References 13 publications

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Mutant of a Xylanase-producing Strain of Aspergillus niger in Solid State Fermentation by Low Energy Ion Implantation

Low-energy Ion Beam Biology Research at Chiang Mai University

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