Mutant of a Xylanase-producing Strain of Aspergillus niger in Solid State Fermentation by Low Energy Ion Implantation

Wu, Min; Li, Shi Chang; Yao, Juanjuan; Pan, Ren Rui; Yu, Zeyang

doi:10.1007/s11274-004-7870-x

Cited by 19 publications

(11 citation statements)

References 11 publications

(8 reference statements)

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“…3 Three-dimensional response surface plot of the second-order regression for rotation-orthogonal composite design xylanase production (5071 IU/g), but used uneconomical 5 days fermentation time. Min Wu et al [4] obtained the highest xylanase production (6,320 IU/g) grown on the material with high hemicellulose content, in which hemicellulose could efficiently induce xylanase [22]. In comparison, inexpensive apple pomace and cotton seed powder were used as basic substrates and the fermentation time was less than 60 h in our studies.…”

Section: Validation Of the Modelmentioning

confidence: 58%

“…Xylanases have been widely applied in food, animal feed, bioconversion, textile, and in paper and pulp industries [3]. However, high cost and low yields of xylanase have been the main problems for its industrial production [4]. Therefore, there is a great need to develop a new fermentation medium with inexpensive substrates that provides a high xylanase yield.…”

Section: Introductionmentioning

confidence: 99%

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Response surface optimization of fermentation conditions for producing xylanase by Aspergillus niger SL-05

Liu

Sun

et al. 2008

J Ind Microbiol Biotechnol

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Fermentation conditions were statistically optimized for producing extracellular xylanase by Aspergillus niger SL-05 using apple pomace and cotton seed meal. The primary study shows that culture medium with a 1:1 ratio of apple pomace and cotton seed meal (carbon and nitrogen sources) yielded maximal xylanase activity. Three significant factors influencing xylanase production were identified as urea, KH(2)PO(4), and initial moisture content using Plackett-Burman design study. The effects of these three factors were further investigated using a design of rotation-regression-orthogonal combination. The optimized conditions by response surface analysis were 2.5% Urea, 0.09% KH(2)PO(4), and 62% initial moisture content. The analysis of variance indicated that the established model was significant (P < 0.05), "while" or "and" the lack of fit was not significant. Under the optimized conditions, the model predicted 4,998 IU/g dry content, whereas validation experiments produced an enzymatic activity of xylanase at 5,662 IU/g dry content after 60 h fermentation. This study innovatively developed a fermentation medium and process to utilize inexpensive agro-industrial wastes to produce a high yield of xylanase.

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Section: Validation Of the Modelmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Response surface optimization of fermentation conditions for producing xylanase by Aspergillus niger SL-05

Liu

Sun

et al. 2008

J Ind Microbiol Biotechnol

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“…, and it was the most common ion used in ion beam implantation [13]. For ion implantation, mutation frequency and screen efficiency are closely related to the energy and the doses of ions [14]. The mechanism including energy absorption, mass deposition, and charge exchange has been proposed by accumulated evidences in breeding, gene transfer, and cell modification [15].…”

Section: Effect Of Nmentioning

confidence: 99%

Screening of lipid high producing mutant from rhodotorula glutinis by low ion implantation and study on optimization of fermentation medium

Zhang

et al. 2013

Indian J Microbiol

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In order to obtain lipid producing strain with high-yield, the wild type stain Rhodotorula glutinis was treated by low ion implantation, and optimization of fermentation medium for higher lipid yield was carried out using mutant strain. It was found that the strain had a higher positive mutation rate when the output power was 10 keV and the dose of N ? implantation was 80 9 2.6 9 10 13 ions/cm 2 . Then a high-yield mutant strain D30 was obtained through cid-heating coupling ultrasonic method and lipid yield was 3.10 g/L. Additionally, the surface response method was used to optimize fermentation medium. The three significant factors (glucose, peptone, KH 2 PO 4 ) were optimized using response surface methodology (RSM), and the optimized parameters of fermentation medium were as follows: glucose 73.40 g/L, peptone 1.06 g/L and KH 2 PO 4 3.56 g/L. Finally the fermentation characteristic of high-yield mutation strain D30 was studied, when fermentation time was 10 days, which lipid yield increased to 7.81 g/L. Fatty acid composition of the lipid was determined by GC, and the most represented fatty acids of mutant D30 were C16:0 (11.4 %), C16:1 (5.66 %), C18:1 (49.3 %), and C18:2 (27.0 %).

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“…In recent years, low-energy ion implantation has been applied to microbiology breeding area, such as vitamin C [32], L-lactic acid [6], xylanase [31], lipopeptide antibiotics [12], etc. According to the experimental results, we may deduce that there may be a new repair mechanism in s65 during ion beam exposure, which has not been discovered and distinguished from the repair of excision, recombination and SOS (errorprone repair).…”

Section: Discussionmentioning

confidence: 99%

Mutation breeding of chitosanase-producing strain Bacillus sp. S65 by low-energy ion implantation

Zhou

et al. 2006

J Ind Microbiol Biotechnol

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In order to obtain an industrial strain with higher chitosanase yield, the wild strain Bacillus sp. S65 cells were mutated by a novel mutagen, nitrogen ion beam, with energy of 15 keV and dose ranging from 2.6 x 10(14 )to 5.2 x 10(15) ions/cm(2). One mutant, s65F5 with high yield of chitosanase was isolated. Results showed that the production of chitosanase of s65F5 was dramatically increased from 4.1 U/ml in s65 to 25 U/ml by ion beam implantation, while the fermentation time was shortened from 72 to 56 h, both of which greatly increased efficiency and reduced the cost of industrial production. Besides, the mutagenic effects of low-energy ion beam on survival rate showed characteristic down-up-down pattern, which was different from the traditional mutagens such as UV and gamma-ray and the possible mutation mechanism was discussed.

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Mutant of a Xylanase-producing Strain of Aspergillus niger in Solid State Fermentation by Low Energy Ion Implantation

Cited by 19 publications

References 11 publications

Response surface optimization of fermentation conditions for producing xylanase by Aspergillus niger SL-05

Response surface optimization of fermentation conditions for producing xylanase by Aspergillus niger SL-05

Screening of lipid high producing mutant from rhodotorula glutinis by low ion implantation and study on optimization of fermentation medium

Mutation breeding of chitosanase-producing strain Bacillus sp. S65 by low-energy ion implantation

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