Iodometric Titration on Microfluidic Paper-Based Analytical Devices for Determination of Ascorbic Acid: A Laboratory Experiment for Chemical Education Undergraduates

Abstract

We describe an iodometric titration method for the determination of ascorbic acid using a microfluidic paper-based analytical device (μPAD). The ascorbic acid was detected by measuring the length of the blue band formed in a straight paper channel delimited by wax. This analytical method was designed as a laboratory experiment for undergraduates majoring in chemical education. The main objectives of this practice are to introduce μPAD into a preservice teacher’s classroom, motivating their passion of learning chemistry, and implementing green chemistry education. Finally, we hope this novel microscale chemistry experiment could be introduced into the chemistry classroom at high schools by these preservice teachers after graduation. A total of 145 preservice chemistry teachers performed this experiment in the fifth semester. They completed this experiment within 3 h; positive feedback from these students indicated that the learning objectives and instructional aims were met.

Ultra-microscale Chemistry Experiment on Microfluidic Devices: A New Trend in Chemical Education

“…Paper-based microfluidic devices have been widely used as educational tools owing to their low cost, rapid fabrication, and ease of operation. Since we piloted an amino acid assay for an undergraduate analytical chemistry course, paper-based devices have been used in teaching laboratories for analysis of nitrite, iron, , copper, antibiotics, ascorbic acid, chemical equilibrium constants, and glucose . Paper-based devices have also been used for teaching some chemical concepts such as acid–base reactions, electrochemistry, and calibration curves .…”

Electrochemical Nucleic Acid Biosensor on a Microfluidic Chip to Understand the Coupling between Electrochemistry and Microfluidics

“…While previously published work focuses mainly on microfluidic chip fabrication, − this article aims at providing in-depth theoretical and practical knowledge of electrochemistry coupled with microfluidics. This laboratory experiment not only shows how flow rates impact electrochemical measurements but also explains the phenomenon behind it (e.g., the impact of forced convection on the diffusion layer and on the biosensor’s detection limit), as well as how the diffusion layer shrinks under forced convection and the consequences on the biosensor’s detection.…”

Enhancing Competency-Based Education in Instrumental Analysis: A Novel Approach Using High-Performance Liquid Chromatography for Real-World Problem Solving

“…One of the current premises in curricula, concerning content related to Instrumental Analysis, is to promote the development of teaching strategies that encourage students to acquire basic skills in the field of chemistry, such as sample preparation, handling of materials, instrumentation, and data analysis. These competencies aim to foster their creativity and autonomy [6], and therefore, new methodologies are considered that enable competency-based education, allowing for comprehensive development of the individual [7]. One of the main objectives of laboratory sessions is for students not only to acquire isolated knowledge of different techniques but also to have the ability to correctly apply the acquired knowledge in solving real analytical problems within their field of study and with autonomy [8].…”