Iodometric Detection of Staphylococcal Penicillinase in Clinical Microbiology

Rg, Cooper; Brown, Gilbert; B, Vesey

doi:10.1038/icb.1966.70

Cited by 7 publications

(4 citation statements)

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“…lodometric methods have successfully detected ,B-lactamase production by various other bacteria (7,8,(13)(14)(15) The identity of each H. influenzae strain was confirmed by its requirements for both hemin (X factor) and nicotinamide adenine dinucleotide (V factor). The immunologic specificity of the type b capsules of all strains was determined by immunofluorescence or by capsular swelling (2).…”

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confidence: 99%

Iodometric Detection of Haemophilus influenzae Beta-Lactamase: Rapid Presumptive Test for Ampicillin Resistance

1975

Antimicrob Agents Chemother

105

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Strains of Haemophilus influenzae type b sporadically isolated from clinical specimens are ampicillin resistant due to production of a f-lactamase. This enzyme which inactivates ampicillin and penicillin G is not produced by ampicillin-susceptible strains. Various characteristics of f-lactamase production and ampicillin resistance of three H. influenzae type b isolates were investigated. A sensitive iodometric test was employed to detect f-lactamase; positive results were obtained in 5 min with 109 bacteria taken from cultures on a nutritionally adequate agar medium. This simple chemical test will enable the hospital laboratory to obtain presumptive evidence of ampicillin resistance on the same day that H. influenzae is isolated.

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confidence: 99%

Iodometric Detection of Haemophilus influenzae Beta-Lactamase: Rapid Presumptive Test for Ampicillin Resistance

1975

Antimicrob Agents Chemother

105

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“…Some virulence factors were detected such as their secretion of the enzyme β-lactamase and the cell wall containing protein A. [ 9 10 ] To confirm isolates as methicillin-resistant, cefoxitin (30 μg) disc diffusion test was performed.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city

Mahdi¹

2023

J Adv Pharm Technol Res

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“…Co., Ltd., Tokyo) per ml. Iodine-potassium iodide solution containing 20,000 U of penicillin per ml was poured onto the colonies produced on the plates, according to an iodometric procedure (2,3), for examination of the Pc-ase production. Colonies producing Pc-ase were surrounded by colorless, clear halos on a dark-blue background, but Pc-ase-deficient colonies were not.…”

Section: Methodsmentioning

confidence: 99%

Mercury and Cadmium Resistances Mediated by the Penicillinase Plasmid in Staphylococcus aureus

1974

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Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat innocuous form. This process is independent of temperature and seems to be controlled by an inducible enzyme. The killing effect of Hg salts was not influenced by the coexistence of other dior monovalent ions such as MgC92, CaCl2, MnCl2, and NaCl. No vaporization of Hg, which explains the resistance mechanism such as that proposed by Komura et al. for R factor-mediated Hg resistance in enterobacilli, was found in the case of Hg resistance in staphylococci. On the other hand, the resistance to Cd ion is mediated by some protective mechanism to retain the ion outside the cell. Pc-sensitive organisms not carrying the Pc-ase plasmid incorporate Cd ions into the cells, whereas the Pc-ase plasmid-carrying organisms do not. The incorporation of this ion is temperature dependent and does not take place at 4 C. When incubated with this ion at 4 C, Pc-sensitive organisms as well as Pc-resistant organisms are also able to show a resistance. The addition of CaCl2 could eliminate the killing effect of CdCl2 with a dose-effective response.

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Iodometric Detection of Staphylococcal Penicillinase in Clinical Microbiology

Cited by 7 publications

References 0 publications

Iodometric Detection of Haemophilus influenzae Beta-Lactamase: Rapid Presumptive Test for Ampicillin Resistance

Iodometric Detection of Haemophilus influenzae Beta-Lactamase: Rapid Presumptive Test for Ampicillin Resistance

Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city

Mercury and Cadmium Resistances Mediated by the Penicillinase Plasmid in Staphylococcus aureus

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