Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine

Elmesiry, Ahmed; Seleim, Magdi; Cullis-Hill, David

doi:10.1016/j.ijvsm.2014.11.001

Cited by 6 publications

(10 citation statements)

References 24 publications

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“…In the present study, lameness was graded according to AAEP, where the highest score was reached after one week then decreased gradually. These results are in agreement with the previous report [34] that lameness was a major feature for MIA-injected donkeys. Also, Uilenreef et al (2019) [37] assessed the lameness in MIA-injected pigs, where the animals showed rapid lameness which was greatly declined by the end of the experiment.…”

Section: Discussionsupporting

confidence: 94%

“…Little of the literature used donkey as the OA model [32][33][34], although it is one of the equine species that is phylogenetically and biochemically closest to the horse, in addition to its low price and low cost of feedstuff and housing [34].…”

Section: Discussionmentioning

confidence: 99%

“…Under strict aseptic conditions, the left radiocarpal joint in each donkey was intra-articularly injected with 25 mg of MIA (sodium monoiodoacetate, Sigma-Aldrich, St. Louis, MO, USA) dissolved in 1 mL of sterile saline [34].…”

Section: Induction Of Osteoarthritismentioning

confidence: 99%

“…Several studies have been elucidated to study OA in horses, especially since they are good models for naturally occurring OA to rescue the horse racing industry and attract attention to human OA [30,31]. Donkey (Equus asinus) is one of the equine species and closest animal to the horse, making this species an alternative cheap animal model for studying equine diseases [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

et al. 2020

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Osteoarthritis (OA) is one of the most degenerative joint diseases in both human and veterinary medicine. The objective of the present study was the early diagnosis of OA in donkeys using a reliable grading of the disease based on clinical, chemical, and molecular alterations. OA was induced by intra-articular injection of 25 mg monoiodoacetate (MIA) as a single dose into the left radiocarpal joint of nine donkeys. Animals were clinically evaluated through the assessment of lameness score, radiographic, and ultrasonographic findings for seven months. Synovial fluid and cartilage samples were collected from both normal and diseased joints for the assessment of matrix metalloproteinases (MMPs) activity, COL2A1 protein expression level, and histopathological and immunohistochemical analysis of Caspase-3. Animals showed the highest lameness score post-induction after one week then decreased gradually with the progression of radiographical and ultrasonographic changes. MMP activity and COL2A1 and Caspase-3 expression increased, accompanied by articular cartilage degeneration and loss of proteoglycan. OA was successfully graded in Egyptian donkeys, with the promising use of COL2A1and Caspase-3 for prognosis. However, MMPs failed to discriminate between early and late grades of OA.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Induction Of Osteoarthritismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

et al. 2020

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“…In addition, monoiodoacetate can be effective in inducing OA in horses just as it is in mice. Elmesirty et al found that injection of 50 mg of mono-iodoacetate into the joint leads to synovitis within a week which subsequently progresses to osteoarthritis within 70 days [55].…”

Section: Limitations Of Murine Oa Models and Use Of Other Animal Modelsmentioning

confidence: 99%

Pros and cons of mouse models for studying osteoarthritis

Bapat¹,

Hubbard²,

Munjal³

et al. 2018

Clinical & Translational Med

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Osteoarthritis (OA) is one of the most common chronic conditions in the world today. It results in breakdown of cartilage in joints and causes the patient to experience intense pain and even disability. The pathophysiology of OA is not fully understood; therefore, there is currently no cure for OA. Many researchers are investigating the pathophysiology of the disease and attempting to develop methods to alleviate the symptoms or cure the OA entirely using animal models. Most studies on OA use animal models; this is necessary as the disease develops very slowly in humans and presents differently in each patient. This makes it difficult to effectively study the progression of osteoarthritis. Animal models can be spontaneous, in which OA naturally occurs in the animal. Genetic modifications can be used to make the mice more susceptible to developing OA. Osteoarthritis can also be induced via surgery, chemical injections, or non-invasive trauma. This review aims to describe animal models of inducing osteoarthritis with a focus on the models used on mice and their advantages and disadvantages that each model presents.

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Morphological and Imaging Evaluation of the Metacarpophalangeal and Metatarsophalangeal Joints in Healthy and Lame Donkeys

El‐Gendy

Alsafy

El-khamary

et al. 2020

Journal of Equine Veterinary Science

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Anatomical description of donkey metacarpophalangeal and metatarsophalangeal joints Pathologies observed in clinically abnormal donkey joints including exostosis New anatomical structures observed in donkey joints Optimized computed tomography methodology can give better visualization for soft tissues Abstract The donkey is of socio-economic value yet imaging techniques in both healthy and abnormal limbs are a limiting factor in research and medicine. The objective was to determine anatomical features of both healthy and clinically abnormal donkey metacarpophalangeal and metatarsophalangeal joints (n=13) using anatomical dissection, casting, x-ray and computed tomography. The joint capsule contained two palmar/plantar and two dorsal recesses. The proximal-palmar or plantar recess was larger than the distodorsal recess and potential sites of approaches to the recesses were determined. Soft tissue structures were distinguished using computed tomography at 300mA which was superior to 120mA. This methodology gave better assessments of the synovial tendon sheath, joint recesses and cruciate, collateral and short sesamoidean ligaments. Computed tomography provided outstanding discrimination between the cortex and medulla of the third metacarpal, the proximal sesamoid bones, the proximal phalanx and excellent details of the osseous structures. Although the joints appeared free from exostosis using x-ray; the position and extension of exostosis in pathologically affected donkeys (a novel finding) was revealed using computed tomography with 300mA in comparison to 120mA. The study also provided an anatomical record of the metacarpophalangeal and metatarsophalangeal joints using the latest technology which could impact on clinical situations including anesthesia injection sites.

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Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine

Cited by 6 publications

References 24 publications

COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

Pros and cons of mouse models for studying osteoarthritis

Morphological and Imaging Evaluation of the Metacarpophalangeal and Metatarsophalangeal Joints in Healthy and Lame Donkeys

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