Iodide enhances IgG synthesis by human peripheral blood lymphocytes in vitro

Weetman, A. P.; McGregor, A M; Campbell, Harold A.; Lazarus, J. H.; Ibbertson, H. K.; Hall, R

doi:10.1530/acta.0.1030210

Cited by 56 publications

(27 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Iodide has also been reported to enhance IgG synthesis by human peripheral blood lymphocytes in vitro. 30 In our study, the detection of low but equivalent proliferative cell responses to Tg and I-Tg in lymphoid organs of NOD. H2 h4 mice developing ISAT additionally suggested that the iodine content of Tg does not affect its antigenicity in this model.…”

Section: Discussionmentioning

confidence: 53%

Iodine content of thyroglobulin in NOD.H2h4 mice developing iodine-accelerated autoimmune thyroiditis

Kolypetri

Noel

Carayanniotis

et al. 2010

View full text Add to dashboard Cite

oBJective: In NOD.H2 h4 mice, high dietary iodine intake has been known to cause Iodineaccelerated spontaneous Autoimmune thyroiditis (IsAt) via an unknown mechanism. the aim of the study was to examine whether the NOD.H2 h4 genetic background predisposes to enhanced iodine organification in thyroglobulin (tg), a target autoantigen in IsAt. DEsIGN: to avoid issues associated with an ongoing anti-tg antibody response, we assessed tg iodination levels in iodine-fed, b-cell deficient NOD.H2 h4 mice. Additionally, we tested whether humoral or cellular immune responses of iodine-fed NOD.H2 h4 mice are preferentially directed to tg with increased iodine content (I-tg) or known pathogenic tg peptides that contained iodine. rEsULts: the iodine content of tg was not significantly different between control (9.0±2.7 I atoms per monomer) and iodine-fed mice (10.9±0.3 I atoms per monomer). Furthermore, in iodine-fed NOD.H2 h4 mice developing IsAt, strong but equivalent serum IgG responses were detected to both tg or I-tg, whereas their lymphoid cells were stimulated weakly but equally well by tg or I-tg in vitro and did not show reactivity against a panel of five pathogenic tg peptides that contained iodine. cONcLUsIONs: the results suggest that development of IsAt in NOD.H2 h4 mice is not associated with enhanced iodine organification or differential b-or t-cell responses to iodinated determinants in tg.

show abstract

Section: Discussionmentioning

confidence: 53%

Iodine content of thyroglobulin in NOD.H2h4 mice developing iodine-accelerated autoimmune thyroiditis

Kolypetri

Noel

Carayanniotis

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Secondly, iodine has a toxic effect on thyroid cells (51)(52)(53). Thirdly, iodine directly stimulates immune and immunity-related cells (54)(55)(56). Then what is the upper limit of iodine intake that could be considered a risk factor for iodine-induced autoimmune thyroiditis in humans?…”

Section: Discussionmentioning

confidence: 99%

More than adequate iodine intake may increase subclinical hypothyroidism and autoimmune thyroiditis: a cross-sectional study based on two Chinese communities with different iodine intake levels

Teng¹,

Shan²,

Chen

et al. 2011

European Journal of Endocrinology

150

View full text Add to dashboard Cite

Objective: With the introduction of iodized salt worldwide, more and more people are exposed to more than adequate iodine intake levels with median urinary iodine excretion (MUI 200-300 mg/l) or excessive iodine intake levels (MUI O300 mg/l). The objective of this study was to explore the associations between more than adequate iodine intake levels and the development of thyroid diseases (e.g. thyroid dysfunction, thyroid autoimmunity, and thyroid structure) in two Chinese populations. Design: A population-based cross-sectional study was conducted in two areas in which people are exposed to different levels of iodine intake (Rongxing, MUI 261 mg/l; Chengshan, MUI 145 mg/l). A total of 3813 individuals were recruited by random sampling. Thyroid hormones, thyroid autoantibodies in serum, and iodine levels in urine were measured. B-mode ultrasonography of the thyroid was also performed for each participant. Results: The prevalence of subclinical hypothyroidism was significantly higher for subjects who live in Rongxing than those who live in Chengshan (5.03 vs 1.99%, P!0.001). The prevalence of positive anti-thyroid peroxidase antibody (TPOAb) and positive anti-thyroglobulin antibody (TgAb) was significantly higher for subjects in Rongxing than those in Chengshan (TPOAb: 10.64 vs 8.4%, PZ0.02; TgAb: 10.27 vs 7.93%, PZ0.01). The increase in thyroid antibodies was most pronounced in the high concentrations of TPOAb (TPOAb: R500 IU/ml) and low concentrations of TgAb (TgAb: 40-99 IU/ml) in Rongxing. Conclusions: More than adequate iodine intake could be a public health concern in terms of thyroid function and thyroid autoimmunity in the Chinese populations.European Journal of Endocrinology 164 943-950

show abstract

“…In addition, I can exhibit pleiotropic effects by influencing processes that interfere with immune function. These include inhibition of IFN-␥-induced class II expression on rat (FRLT-5), but not human, thyroid cells (49), increased inducibility of the 72-kDa heat shock protein in cultured human thyrocytes (50), and enhancement of IgG production in pokeweed mitogen-stimulated human peripheral blood lymphocytes in vitro (51).…”

Section: Discussionmentioning

confidence: 99%

Enhanced Iodination of Thyroglobulin Facilitates Processing and Presentation of a Cryptic Pathogenic Peptide

Dai

Rao

Carayanniotis

2002

The Journal of Immunology

View full text Add to dashboard Cite

Increased iodine intake has been associated with the development of experimental autoimmune thyroiditis (EAT), but the biological basis for this association remains poorly understood. One hypothesis has been that enhanced incorporation of iodine in thyroglobulin (Tg) promotes the generation of pathogenic T cell determinants. In this study we sought to test this by using the pathogenic nondominant As-binding Tg peptides p2495 and p2694 as model Ags. SJL mice challenged with highly iodinated Tg (I-Tg) developed EAT of higher severity than Tg-primed controls, and lymph node cells (LNC) from I-Tg-primed hosts showed a higher proliferation in response to I-Tg in vitro than Tg-primed LNC reacting to Tg. Interestingly, I-Tg-primed LNC proliferated strongly in vitro against p2495, but not p2694, indicating efficient and selective priming with p2495 following processing of I-Tg in vivo. Tg-primed LNC did not respond to either peptide. Similarly, the p2495-specific, IL-2-secreting T cell hybridoma clone 5E8 was activated when I-Tg-pulsed, but not Tg-pulsed, splenocytes were used as APC, whereas the p2694-specific T cell hybridoma clone 6E10 remained unresponsive to splenic APC pulsed with Tg or I-Tg. The selective in vitro generation of p2495 was observed in macrophages or dendritic cells, but not in B cells, suggesting differential processing of I-Tg among various APC. These data demonstrate that enhanced iodination of Tg facilitates the selective processing and presentation of a cryptic pathogenic peptide in vivo or in vitro and suggest a mechanism that can at least in part account for the association of high iodine intake and the development of EAT.

show abstract

Iodide enhances IgG synthesis by human peripheral blood lymphocytes in vitro

Cited by 56 publications

References 8 publications

Iodine content of thyroglobulin in NOD.H2h4 mice developing iodine-accelerated autoimmune thyroiditis

Iodine content of thyroglobulin in NOD.H2h4 mice developing iodine-accelerated autoimmune thyroiditis

More than adequate iodine intake may increase subclinical hypothyroidism and autoimmune thyroiditis: a cross-sectional study based on two Chinese communities with different iodine intake levels

Enhanced Iodination of Thyroglobulin Facilitates Processing and Presentation of a Cryptic Pathogenic Peptide

Contact Info

Product

Resources

About