Involvement of zinc in intracellular oxidant/antioxidant balance

Parat, Marie-Odile; Richard, Marie‐Jeanne; Béani, J.-C.; Favier, A.

doi:10.1007/bf02784439

Cited by 69 publications

(38 citation statements)

References 38 publications

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“…We studied Zn-related detoxification factors, such as metallothioneins (17), GSH (18), and the GSH-coupled excretory pump GS-X (4), and we observed that intracellular GSH was high in PC/RES cells (Fig. 3A).…”

Section: Resultsmentioning

confidence: 99%

Element Array by Scanning X-ray Fluorescence Microscopy after Cis-Diamminedichloro-Platinum(II) Treatment

Shimura

Saitô

Matsuyama³

et al. 2005

Cancer Research

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Minerals are important for cellular functions, such as transcription and enzyme activity, and are also involved in the metabolism of anticancer chemotherapeutic compounds. Profiling of intracellular elements in individual cells could help in understanding the mechanism of drug resistance in tumors and possibly provide a new strategy of anticancer chemotherapy. Using a recently developed technique of scanning X-ray fluorescence microscopy (SXFM), we analyzed intracellular elements after treatment with cisdiamminedichloro-platinum(II) (CDDP), a platinum-based anticancer agent. The images obtained by SXFM (element array) revealed that the average Pt content of CDDP-resistant cells was 2.6 times less than that of sensitive cells, and the zinc content was inversely correlated with the intracellular Pt content. Data suggested that Zn-related detoxification is responsible for resistance to CDDP. Of Zn-related excretion factors, glutathione was highly correlated with the amount of Zn. The combined treatment of CDDP and a Zn(II) chelator resulted in the incorporation of thrice more Pt with the concomitant down-regulation of glutathione. We propose that the generation of an element array by SXFM opens up new avenues in cancer biology and treatment. (Cancer Res 2005; 65(12): 4998-5002)

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Section: Resultsmentioning

confidence: 99%

Element Array by Scanning X-ray Fluorescence Microscopy after Cis-Diamminedichloro-Platinum(II) Treatment

Shimura

Saitô

Matsuyama³

et al. 2005

Cancer Research

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“…Zn has catalytic and structural roles in more than 300 metalloenzymes, as well as regulatory roles in diverse cellular processes such as signaling transduction and gene expression (McCall et al, 2000). Many studies have demonstrated that Zn per se functions as antioxidant and also plays an important role in regulation of cellular glutathione (GSH) (Parat et al, 1997;Powell, 2000;Zhou et al, 2005). Although GSH is the most important molecule involved in cellular antioxidant defense, radical scavenging activity of GSH is 300-times lower than that of MT (Thornalley and Vasak, 1985).…”

Section: Effect Of Zn and CD Against Acute Ccl 4 Toxicitymentioning

confidence: 99%

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

et al. 2016

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-Carbon tetrachloride (CCl 4 ) is commonly used as a chemical inducer of experimental liver injury. Several compounds have been demonstrated to attenuate the hepatic damage caused by sublethal doses of CCl 4 . However, rescue from lethal toxicity of CCl 4 has not been reported. In the present study, we evaluated the protective effect of metallothionein (MT), an endogenous scavenger of free radicals, on CCl 4 -induced lethal toxicity of mice. To induce MT production in male ddY mice, we administered Zn (as ZnSO 4 ) at 50 mg/kg as a once-daily subcutaneous injection for 3 days prior to a single intraperitoneal administration of 4 g/kg CCl 4 . Animals were observed for mortality every 3 hr for 24 hr after CCl 4 injection. Liver damage was assessed by determining (in a subset of these mice) blood levels of alanine aminotransferase (ALT; a marker of liver injury) and liver histopathology at 6 hr after CCl 4 injection. Our results showed that three times pretreatment with Zn yielded > 40-fold induction of hepatic MT protein levels compared to control group. Zn pretreatment completely abolished the CCl 4 -induced mortality of mice. We also found that pretreatment of mice with Zn significantly decreased the ALT levels and reduced the histological liver damage as assessed at 6 hr post-CCl 4 . These findings suggest that prophylaxis with Zn protects mice from CCl 4 -induced acute hepatic toxicity and mortality, presumably by induction of radical-scavenging MT.

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“…Radikal bebas dan senyawa oksigen reaktif yang diproduksi dalam jumlah yang normal, penting untuk fungsi biologis, namun ia tidak menyerang sasaran spesifik, sehingga ia juga akan menyerang asam lemak tidak jenuh ganda dari membran sel, organel sel atau deoxyribonucleid acid (DNA), hal ini dapat menyebabkan kerusakan struktur dan fungsi sel (Winarsi, 2007). Lebih lanjut Parat et al (1997) menyatakan bahwa stres oksidatif dapat mengakibatkan terjadinya kematian sel (nekrosis). Menurunnya jumlah eritrosit pada perlakuan pada P2 dan P3 diduga karena adanya nekrosis atau kerusakan jaringan pada organ pembentukan sel-sel darah pada ikan nila.…”

Section: Tabel 1 Rata-rata (±Sd) Jumlah Eritrosit Ikan Nila Pada Masunclassified

PENGARUH PENINGKATAN SUHU TERHADAP JUMLAH ERITROSIT IKAN NILA (Oreochromis niloticus)

Bangsa¹,

Sugito²,

Zuhrawati³

et al. 2015

J.Med.Vet.

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ABSTRAKPenelitian ini bertujuan mengetahui pengaruh peningkatan suhu terhadap jumlah eritrosit ikan nila. Sampel yang digunakan adalah darah ikan nila yang berasal dari 18 ekor ikan nila dengan bobot badan berkisar 40-50 g. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) pola searah dengan tiga perlakuan dan enam kali ulangan. Perlakuan P1 sebagai kontrol merupakan perlakuan dengan suhu akuarium 29±1 C, P2 merupakan perlakuan dengan suhu akuarium 32±1 C, dan P3 merupakan perlakuan dengan suhu akuarium 35±1 C. Ukuran akuarium pada masing-masing kelompok perlakuan adalah 80 x 60 x 40 cm dengan ketinggian air 30 cm. Perlakuan dilakukan selama 15 hari dan pada hari ke-16 dilakukan pengambilan sampel darah. Darah diambil melalui vena caudalis. Data dianalisis dengan analisis varian (ANAVA) dan dilanjutkan dengan uji Duncan. Rata-rata (±SD) jumlah eritrosit ikan nila (10 6 /mm 3 ) pada P1, P2, dan P3 masing-masing adalah 3,01x10 6 /mm 3 ; 2,63x10 6 /mm 3 ; dan 2,08x10 6 /mm 3 . Hasil penelitian menunjukkan bahwa peningkatan suhu berpengaruh (P<0,05) terhadap jumlah eritrosit ikan nila. Dapat disimpulkan bahwa peningkatan suhu air 32±1 C dan 35±1 C dalam akuarium dapat menurunkan jumlah eritrosit ikan nila. ____________________________________________________________________________________________________________________ Kata kunci: suhu, eritrosit, ikan nila ABSTRACT The aim of this research was to find the effect of temperature increase on erythrocytes count of tilapia. The blood from 18 tilapia weighing from 40 to 50 g were used in this study and divided into 3 groups. The research method used was a laboratory experiment. The research was conducted using completely randomized design (CRD) one way pattern with 3 treatments and 6 replications. Group P1 was untreated control with aquarium temperature 29±1 C, P2 was the treatment with aquarium temperature 32±1 C, and P3 was the treatment with aquarium PENDAHULUANIkan nila (Oreochromis niloticus) dapat hidup di lingkungan air tawar, air payau, dan air asin (Suyanto, 2005). Ikan yang berasal dari Sungai Nil ini telah dibudidayakan di berbagai negara di dunia, termasuk di Indonesia (Kordi, 2010). Budidaya ikan nila mempunyai prospek yang baik untuk dikembangkan di Indonesia, karena budidayanya dapat dilakukan di tambak, lahan bekas galian pasir atau penambangan, dan karamba jaring apung (KJA) di perairan umum atau laut. Potensi ikan nila untuk dibudidayakan cukup besar karena memiliki beberapa kelebihan, yaitu mudah berkembang biak, pertumbuhan cepat, kandungan protein cukup tinggi, ukuran tubuh relatif besar, tahan terhadap penyakit, mudah beradaptasi dengan lingkungan, harga relatif murah, dan mempunyai nilai gizi yang cukup tinggi sebagai sumber protein hewani (Wardoyo, 2005).Menurut Syahailatua (2008), dampak perubahan iklim yang diakibatkan meningkatnya suhu udara di bumi tentu cukup mengkhawatirkan bagi kehidupan manusia. Selama 50 tahun terakhir, suhu atmosfir bumi terus meningkat, yang secara langsung kondisi ini juga menaikkan suhu...

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Involvement of zinc in intracellular oxidant/antioxidant balance

Cited by 69 publications

References 38 publications

Element Array by Scanning X-ray Fluorescence Microscopy after Cis-Diamminedichloro-Platinum(II) Treatment

Element Array by Scanning X-ray Fluorescence Microscopy after Cis-Diamminedichloro-Platinum(II) Treatment

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

PENGARUH PENINGKATAN SUHU TERHADAP JUMLAH ERITROSIT IKAN NILA (Oreochromis niloticus)

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