Involvement of Wnt activation in the micromechanical vibration-enhanced osteogenic response of osteoblasts

Hou, Wanwan; Zhu, Zhuo Li; Zhou, Yi; Zhang, Chun Xiang; Yu, Hai

doi:10.1007/s00776-011-0124-5

Cited by 26 publications

(31 citation statements)

References 30 publications

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“…Vibration plates have been and are being used to enhance bone formation. However, there had been no studies in cases of distraction osteogenesis in humans (Hou et al, 2011).…”

Section: Vibrationsmentioning

confidence: 99%

Distraction Osteogenesis and Its Challenges in Bone Regeneration

Hamdy¹,

Rendon²,

Tabrizian³

2012

Bone Regeneration

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“…Vibration plates have been and are being used to enhance bone formation. However, there had been no studies in cases of distraction osteogenesis in humans (Hou et al, 2011).…”

Section: Vibrationsmentioning

confidence: 99%

Distraction Osteogenesis and Its Challenges in Bone Regeneration

Hamdy¹,

Rendon²,

Tabrizian³

2012

Bone Regeneration

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“…The vibration technique used in these studies was also different. Some vibrated the culture plates perpendicularly (Hou et al 2011), while others applied vibration horizontally (Bacabac et al 2006;Rosenberg et al 2002;Tjandrawinata et al 1997). The manner by which vibrational strain was applied to cells on a plastic substrate is different.…”

Section: Introductionmentioning

confidence: 99%

Vibrational stimulation induces osteoblast differentiation and the upregulation of osteogenic gene expression in vitro

2016

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Vibrational stimulation is an accepted non-invasive method used to improve bone remodeling. However, the underlying mechanisms of this phenomenon remain unclear. In this study, we developed a new vibration-loading system to apply vibrational stimulation to cells based on a previously reported in vivo study. We hypothesized that osteoblasts respond to vibrational strain by expressing osteogenic marker genes, such as alkaline-phosphatase (ALP), Runx2, and Osterix. To test our hypothesis, we developed a vibration-loading system to apply a precise vibrational force to an osteoblast culture on a silicone membrane. The system regulated frequency and acceleration of the vibration, and strain on the silicone membrane culture surface was measured using the strain gauge method. After vibrational stimulation, cellular gene expression was analyzed using real-time polymerase chain reaction. We obtained clear strain signals from the culture surface at vibrational ranges of 1.0-10 m/s 2 acceleration and frequencies of 30, 60, and 90 Hz, respectively. The strain increased in a linear fashion, depending on the acceleration magnitude. Vibrational stimulation also significantly upregulated expression of the osteogenic marker genes Runx2, Osterix, type I collagen, and ALP. In conclusion, we developed a new vibrationloading system that can precisely regulate frequency and acceleration, and we established the presence of dynamic cellular strain on a culture surface. Our findings suggest that vibrational stimulation may directly induce osteoblast differentiation.

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“…The vibration frequency was 40 Hz, while the magnitude was set to 0.06, 0.14, 0.32, 0.49, 0.66 or 0.8 g as previous studies indicated these values to be optimal for bone remodeling (Hou et al, 2011;Judex, Lei, Han, & Rubin, 2007;Xie et al, 2006;Zhou et al, 2011). Twelve hours after a 3-day vibration treatment (30 min/day), cells were harvested for assessing biochemical markers.…”

Section: Cell Culture and Lmhf Vibrationsmentioning

confidence: 99%

“…MC3T3-E1 cells (obtained from the State Key Laboratory of Oral Diseases at Sichuan University) were cultured and treated with LMHF vibrations as previously described (Hou et al, 2011). The vibration frequency was 40 Hz, while the magnitude was set to 0.06, 0.14, 0.32, 0.49, 0.66 or 0.8 g as previous studies indicated these values to be optimal for bone remodeling (Hou et al, 2011;Judex, Lei, Han, & Rubin, 2007;Xie et al, 2006;Zhou et al, 2011).…”

Section: Cell Culture and Lmhf Vibrationsmentioning

confidence: 99%

“…Although the micromechanical environment is involved in alveolar bone remodeling and is important for implant osteointegration, there is little information regarding the molecular response of cells to micromovement forces. Our previous study showed that a LMHF mechanical vibration of 0.49 g best activates Wnt10b and promotes osteogenesisinMC3T3-E1 cells (Hou, Zhu, Zhou, Zhang, & Yu, 2011). In addition, LMHF vibrations promote bone marrow stromal cell (BMSC) differentiation and increase bone formation from BMSC progenitors seeded on bone-derived scaffolds along with increased expression ofCbfa1, ALP, collagen I and osteocalcin .…”

Section: Introductionmentioning

confidence: 98%

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