Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components

Doke, Noriyuki

doi:10.1016/0048-4059(83)90019-x

Cited by 728 publications

(358 citation statements)

References 35 publications

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“…We used nitro blue tetrazolium (NBT) salt, a chromogenic redox indicator, to demonstrate the generation and release of superoxide anion [36]. We used 3,3′-diaminobenzidine (DAB), to reveal hydrogen peroxide production [37] and we used the fluorogenic probe, 2′,7′-dichlorodihydrofluorescin diacetate (DCFH-DA), to detect hydroperoxides inside the cells [38].…”

Section: Detection Of Ros (Superoxide Hydrogen Peroxide and Hydropementioning

confidence: 99%

Phytotoxicity of multi-walled carbon nanotubes on red spinach (Amaranthus tricolor L) and the role of ascorbic acid as an antioxidant

Begum

Fugetsu

2012

Journal of Hazardous Materials

170

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Carbon nanotubes (CNTs) are a novel nanomaterial with wide potential applications; however the adverse effects of CNTs following environmental exposure have recently received significant attention. Herein, we explore the systemic toxicity and potential influence of 0-1000 mg L −1 the multi-walled CNTs on red spinach. CNTs exposed plants exhibited growth inhibition and cell death after 15 days of hydroponic culture.CNTs had adverse effects on root and leaf morphology, as observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Raman spectroscopy detected CNTs in leaves. Biomarkers of nanoparticle toxicity, reactive oxygen species (ROS), and cell damage in the red spinach were greatly increased 15 days post-exposure to CNTs. These effects were reversed when CNTs were supplemented with ascorbic acid (AsA), suggesting a role of ROS in the CNT-induced toxicity and that the primary mechanism of CNTs' toxicity is oxidative stress.

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Section: Detection Of Ros (Superoxide Hydrogen Peroxide and Hydropementioning

confidence: 99%

Phytotoxicity of multi-walled carbon nanotubes on red spinach (Amaranthus tricolor L) and the role of ascorbic acid as an antioxidant

Begum

Fugetsu

2012

Journal of Hazardous Materials

170

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“…During the oxidative burst hydrogen peroxide is generated with concomitant oxygen consumption, which mediates rapid crosslinking and insolubilization of the cell-wall structural proteins presumably making the damaged site invulnerable for further penetration of pathogens to interior (Bradley et al, 1992;Levine et al, 1994). Very little is known about the mechanism and origin of oxidative burst in plants in spite of the intensive research since its discovery by Doke in 1983(Doke, 1983. The plant defense response related genes characterized to date fail to identify features that could be related to the redox active enzymes or proteins capable of reducing molecular oxygen.…”

Section: Stellacyanin Plastocyaninmentioning

confidence: 99%

Uclacyanins, stellacyanins, and plantacyanins are distinct subfamilies of phytocyanins: Plant‐specific mononuclear blue copper proteins

et al. 1998

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The cDNAs encoding plantacyanin from spinach were isolated and characterized. In addition, four new cDNA sequences from Arubidopsis ESTs were identified that encode polypeptides resembling phytocyanins, plant-specific proteins constituting a distinct family of mononuclear blue copper proteins. One of them encodes plantacyanin from Arubidopsis. while three others, designated as uclacyanin 1, 2, and 3, encode protein precursors that are closely related to precursors of stellacyanins and a blue copper protein from pea pods. Comparative analyses with known phytocyanins allow further classification of these proteins into three distinct subfamilies designated as uclacyanins, stellacyanins, and plantacyanins. This specification is based on (1) their spectroscopic properties, (2) their glycosylation state, (3) the domain organization of their precursors, and (4) their copper-binding amino acids. The recombinant copper binding domain of Arubidopsis uclacyanin I was expressed, purified, and shown to bind a copper atom in a fashion known as "blue" or type 1. The mutant of cucumber stellacyanin in which the glutamine axial ligand was substituted by a methionine (Q99M) was purified and shown to possess spectroscopic properties similar to uclacyanin I rather than to plantacyanins. Its redox potential was determined by cyclic voltammetry to be +420 mV, a value that is significantly higher than that determined for the wild-type protein (+260 mV). The available structural data suggest that stellacyanins (and possibly other phytocyanins) might not be diffusible electron-transfer proteins participating in long-range electron-transfer processes. Conceivably, they are involved in redox reactions occurring during primary defense responses in plants and/or in lignin formation.

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“…In 1983, Doke reported a production of O 2 Å during an incompatible interaction of potato with the oomycete Phytophthora infestans (Doke, 1983). This observation set forth a considerable wave of studies on the production of ROS in whole plants or in suspension cells confronted with live pathogens or various elicitors.…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components

Cited by 728 publications

References 35 publications

Phytotoxicity of multi-walled carbon nanotubes on red spinach (Amaranthus tricolor L) and the role of ascorbic acid as an antioxidant

Phytotoxicity of multi-walled carbon nanotubes on red spinach (Amaranthus tricolor L) and the role of ascorbic acid as an antioxidant

Uclacyanins, stellacyanins, and plantacyanins are distinct subfamilies of phytocyanins: Plant‐specific mononuclear blue copper proteins

Reactive oxygen species and plant resistance to fungal pathogens

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