Involvement of polyamines in epidermal growth factor (EGF), transforming growth factor (TGF)-α and -β1 action on culture of L6 and fetal bovine myoblasts

Blachowski, S.; Motyl, T.; Grzelkowska, K.; Kasterka, M.; Orzechowski, Arkadiusz; Interewicz, Boźena

doi:10.1016/0020-711x(94)90082-5

Cited by 20 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These molecules may stimulate polyamines (27) which, in turn, may affect these same molecules and their receptors (28) and alter phosphorylation of retinoblastoma protein (29), which is integral to the G1-S phase transition (22). Furthermore, transforming growth factor-␤ might also inhibit phosphorylation of retinoblastoma protein, thereby arresting progression through the cell cycle and converting hyperplasia into hypertrophy (22).…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Ornithine Decarboxylase in Distal Tubules of Early Diabetic Rat Kidneys

et al. 2003

View full text Add to dashboard Cite

Polyamines are small biogenic molecules that are essential for cell cycle entry and progression and proliferation. They can also contribute to hypertrophy. The activity of ornithine decarboxylase (ODC), the ratelimiting enzyme in polyamine biosynthesis, increases in the early diabetic kidney to enable renal hypertrophy. Inhibition of ODC in early diabetes attenuates diabetic renal hypertrophy and glomerular hyperfiltration. The current studies examine the temporal profile of renal ODC protein expression and localization, intrarenal polyamine levels, and sites of proliferation in kidneys of rats during the first 7 days of streptozotocin diabetes. ODC mRNA and protein content were increased in diabetic kidneys. High-performance liquid chromatography analysis showed increased intrarenal polyamine concentrations peaking after 24 h of diabetes. A subsequent increase in the number of proliferating proximal tubular cells was detected by in vivo 5-bromodeoxyuridine (BrdU) incorporation on day 3. Surprisingly, immunohistochemical studies revealed that increased ODC protein was apparent only in distal nephrons, whereas the main site of diabetic kidney hypertrophy is the proximal tubule. These findings raise the possibility that polyamines produced in the distal nephron may mediate the early diabetic kidney growth of the proximal tubules via a paracrine mechanism.

show abstract

Section: Discussionmentioning

confidence: 99%

Increased Expression of Ornithine Decarboxylase in Distal Tubules of Early Diabetic Rat Kidneys

et al. 2003

View full text Add to dashboard Cite

show abstract

“…The maintenance of appropriate levels of intracellular polyamines is crucial to the balance between cell growth and differentiation, since polyamines mediate DNA synthesis, cell cycle events, and apoptosis via a variety of mechanisms that include interactions with key regulatory factors including the estrogen receptor [8], control of growth factor secretion [9] and action [10], and induced expression of immediate early and cyclin genes [11,12]. Indeed, overexpression of human SSAT in Escherichia coli retarded cellular growth, while addition of polyamines to these cells abrogated the effect of increased SSAT [13].…”

Section: Introductionmentioning

confidence: 99%

Paracrine Inducers of Uterine Endometrial Spermidine/Spermine N1-Acetyltransferase Gene Expression during Early Pregnancy in the Pig1

Green¹,

Chung²,

Reed³

et al. 1998

Biology of Reproduction

View full text Add to dashboard Cite

The endogenous factors that underlie the transient induction of the gene encoding spermidine/spermine N1-acetyltransferase (SSAT), the rate-limiting enzyme in cellular polyamine catabolism, in pig uterine endometrium during periimplantation are not known. The present study examined a number of peptide growth factors and regulatory molecules that are present within the uterine environment at early pregnancy, coincident with maximal SSAT gene expression, for their ability to manifest endogenous SSAT gene-inducing activity. Basal SSAT expression in luminal epithelial cells was higher (p < 0. 01) than that for glandular epithelial (GE) or stromal (ST) cells. Recombinant human insulin-like growth factor-I (IGF-I; 50 ng/ml) had no effect on steady-state SSAT mRNA levels, but it increased mitogenesis in all three cell types. In contrast, IGF-I caused a marked induction (p < 0.01) of SSAT mRNA levels in the human endometrial carcinoma cell line Hec-1-A. Uterine explants incubated with interleukin-6, transforming growth factor alpha, epidermal growth factor (each at 1, 10, and 100 ng/ml), retinoic acid and retinol (each at 0.01, 0.1, and 1 microM), and estradiol-17beta (10 nM) had SSAT mRNA levels similar to controls. By contrast, leukemia inhibitory factor (LIF; at 10 and 100 ng/ml) caused a modest, but significant (p < 0.05), increase in SSAT mRNA levels over those of untreated explants. This effect of LIF, however, did not approach the level of induction observed in GE or ST cells after addition of medium conditioned by Day 12 or 17 porcine conceptuses and in endometrial explants supplemented with medium conditioned by Day 21 porcine conceptuses or a continuous cell line (Jag-1) derived from Day 14 porcine trophoblast. We suggest that transient induction of endometrial SSAT gene expression at implantation is mediated by the functional interactions of specific conceptus-derived regulatory factors, distinct from estrogen, with endometrial-derived factor(s) such as LIF. These complex interactions are probably requisite for the transient, yet dramatic, induction of SSAT gene expression and may be critical for successful implantation.

show abstract

“…Indeed, in vitro studies have shown that the application of EGF (epidermal growth factor) can either lead to a reduction in the activity of ODC and AdoMetDC [22] or stimulation [23]. Moreover, activation of dopamine and adrenergic receptors by selective agonists can induce ODC expression in normal female mouse kidney [24].…”

Section: Regulation Of the Expression Of Enzymes Involved In Polyaminmentioning

confidence: 98%

Nuclear and membrane receptor-mediated signalling pathways modulate polyamine biosynthesis and interconversion

Grzelakowska-Sztabert

Dudkowska

Manteuffel-Cymborowska

2007

Biochemical Society Transactions

View full text Add to dashboard Cite

Polyamines play an important role in cell growth and differentiation, while their overproduction has potentially oncogenic consequences. Polyamine homoeostasis, a critical determinant of cell fate, is precisely tuned at the level of biosynthesis, degradation and transport. The enzymes ODC (ornithine decarboxylase), AdoMetDC (S-adenosylmethionine decarboxylase) and SSAT (spermidine/spermine N(1)-acetyltransferase) are critical for polyamine pool maintenance. Our experiments were designed to examine the expression of these enzymes in testosterone-induced hypertrophic and antifolate-induced hyperplastic mouse kidney, characterized by activation of AR (androgen receptor) and HGF (hepatocyte growth factor) membrane receptor c-Met respectively. The expression of these key enzymes was up-regulated by antifolate CB 3717 injury-evoked activation of HGF/c-Met signalling. In contrast, activation of the testosterone/AR pathway remarkably induced a selective increase in ODC expression without affecting other enzymes. Studies in catecholamine-depleted kidneys point to a synergistic interaction between the signalling pathways activated via cell membrane catecholamine receptors and AR, as well as c-Met. We found that this cross-talk modulated the expression of ODC and AdoMetDC, enzymes limiting polyamine biosynthesis, but not SSAT. This is in contrast with the antagonistic cross-talk between AR- and c-Met-mediated signalling which negatively regulated the expression of ODC, but affected neither AdoMetDC nor SSAT.

show abstract

Involvement of polyamines in epidermal growth factor (EGF), transforming growth factor (TGF)-α and -β1 action on culture of L6 and fetal bovine myoblasts

Cited by 20 publications

References 30 publications

Increased Expression of Ornithine Decarboxylase in Distal Tubules of Early Diabetic Rat Kidneys

Increased Expression of Ornithine Decarboxylase in Distal Tubules of Early Diabetic Rat Kidneys

Paracrine Inducers of Uterine Endometrial Spermidine/Spermine N1-Acetyltransferase Gene Expression during Early Pregnancy in the Pig1

Nuclear and membrane receptor-mediated signalling pathways modulate polyamine biosynthesis and interconversion

Contact Info

Product

Resources

About