Involvement of PI3K/Akt signaling pathway in promoting osteogenesis on titanium implant surfaces modified with novel non-thermal atmospheric plasma

Abstract

Non-thermal atmospheric plasma (NTAP) modification to induce a hydrophilic titanium (Ti) surface with less carbon contamination, has been demonstrated to boost the osteogenic responses. In this study, we investigated the underlying bone formation mechanism of NTAP-Ti, and the involvement of PI3K/Akt signaling pathway in regulating osteogenic activities on NTAP-Ti surfaces. NTAP was employed for Ti activation, and PI3K inhibitor, LY294002, was applied to the suppression of PI3K/Akt pathway. We systematically and quantitatively detected the cell morphology, attachment, proliferation, osteogenic differentiation and mineralization of MC3T3-E1 mouse preosteoblasts, and molecular expressions involved in osteogenesis and PI3K/Akt signaling pathway in vivo and in vitro. A descent in osteoblast proliferation on Ti surfaces in relation to LY294002. Alkaline phosphatase (ALP) activity, as well as matrix mineralization, was mitigated by PI3K inhibitor in NTAP-Ti. Likewise, the expression levels of osteogenesis-related genes [ALP, osteocalcin (Ocn), osteopontin (Opn) and runt-related transcription factor 2 (Runx2)] on NTAP-Ti were notably attenuated by LY294002, as confirmed by the results of osteogenesis-related proteins (ALP, and Runx2) expression analysis. In addition, the expression of PI3K/Akt signal pathway proteins further verified the inhibition of LY294002 on Ti surfaces modified by NTAP. Collectively, the PI3K/Akt signal pathway was involved in the amelioration of osteogenesis induced by NTAP modification. NTAP treatment for Ti activation is promising in augmented osteogenic potential through the activation of PI3K/Akt signal pathway.

Role of Berberine Thermosensitive Hydrogel in Periodontitis via PI3K/AKT Pathway In Vitro

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Novel Nonthermal Atmospheric Plasma Irradiation of Titanium Implants Promotes Osteogenic Effect in Osteoporotic Conditions

“…33 In addition, previous studies showed that NTAP-treated implants promoted the biological behavior of rat bone marrow mesenchymal stem cells (BMSCs) and MC3T3-E1. 34,35 Previous comparative experiments also revealed that the osteogenic effect of the NTAP-treated implant was similar to that of the Straumann SLActive implant. 36 In summary, the NTAP device boasts highly efficient, convenient, and biologically safe advantages.…”

Melatonin-encapsuled silk fibroin electrospun nanofibers promote vascularized bone regeneration through regulation of osteogenesis-angiogenesis coupling

“…In accordance with our findings, Ren et al observed the activation of the PI3K/AKT signaling pathway in the melatonin-induced preservation of the osteogenic potential of MC3T3-E1 preosteoblast cells against iron overload [ 40 ]. Conversely, the inhibition of PI3K phosphorylation by LY294002 hindered the osteointegration of titanium implant surfaces treated with innovative non-thermal atmospheric plasma, leading to a decline in osteoblast proliferation and differentiation [ 41 ]. The participation of the PI3K/AKT pathway has been previously validated in a study where the inhibition of PI3K resulted in the elimination of the bone regenerative impact of nano-laponite hydrogel, as demonstrated by the attenuation of calcified matrix formation.…”