Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants

Profotová, Bronislava; Burketová, Lenka; Novotná, Zuzana; Martinec, Jan; Valentová, Olga

doi:10.1016/j.plaphy.2006.02.003

Cited by 46 publications

(28 citation statements)

References 45 publications

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“…Such oxidative bursts are the first reactions of plants to avirulent pathogens (Xu et al, 2005). Activation of PLC and PLD activities in vitro was described after SA treatment of Brassica napus (Profotová et al, 2006). We have recently reported that SA treatment triggers a lipid signaling pathway.…”

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confidence: 98%

Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions

et al. 2009

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Salicylic acid (SA) plays a central role in defense against pathogen attack, as well as in germination, flowering, senescence, and the acquisition of thermotolerance. In this report we investigate the involvement of phospholipase D (PLD) in the SA signaling pathway. In presence of exogenous primary alcohols, the production of phosphatidic acid by PLD is diverted toward the formation of phosphatidylalcohols through a reaction called transphosphatidylation. By in vivo metabolic phospholipid labeling with 33 P i , PLD activity was found to be induced 45 min after addition of SA. We show that incubation of Arabidopsis (Arabidopsis thaliana) cell suspensions with primary alcohols inhibited the induction of two SA-responsive genes, PATHO-GENESIS-RELATED1 and WRKY38, in a dose-dependent manner. This inhibitory effect was more pronounced when the primary alcohols were more hydrophobic. Secondary or tertiary alcohols had no inhibitory effect. These results provide compelling arguments for PLD activity being upstream of the induction of these genes by SA. A subsequent study of n-butanol effects on the SA-responsive transcriptome identified 1,327 genes differentially expressed upon SA treatment. Strikingly, the SA response of 380 of these genes was inhibited by n-butanol but not by tert-butanol. A detailed analysis of the regulation of these genes showed that PLD could act both positively and negatively, either on gene induction or gene repression. The overlap with the previously described phosphatidylinositol-4-kinase pathway is discussed.

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confidence: 98%

Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions

et al. 2009

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“…B. napus was sprayed with 50 mM SA (Sigma, MO, USA (Hegedus et al 2008)), 20 µM JA (Sigma (Profotova et al 2006)), or 20 µM ACC (Sigma (Chen and Chang 2003)). Ethanol in water (0.1% (v/v)) was used as a control for ACC treatment, and water was used as a control for both SA and JA treatments.…”

Section: Plant Growth and Treatmentsmentioning

confidence: 99%

“…Ethanol in water (0.1% (v/v)) was used as a control for ACC treatment, and water was used as a control for both SA and JA treatments. Leaves were harvested at 2, 4, 8, 24 h post-treatment (Hegedus et al 2008;Profotova et al 2006;Chen and Chang 2003), flash-frozen in liquid nitrogen and stored at −80°C.…”

Section: Plant Growth and Treatmentsmentioning

confidence: 99%

Characterization of Defense Signaling Pathways of Brassica napus and Brassica carinata in Response to Sclerotinia sclerotiorum Challenge

et al. 2009

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Canola (Brassica napus L.) is an agriculturally and economically important crop in Canada, and its growth and yield are frequently influenced by fungal pathogens. Sclerotinia sclerotiorum is among those fungal pathogens and causes stem rot disease in B. napus whereas it has been reported that Brassica carinata is moderately tolerant to S. sclerotiorum. Jasmonic acid/ethylene (JA/ET) and salicylic acid (SA) are phytohormones that are known to be involved in plant disease responses. To investigate the defense signaling cascades involved in the interaction of B. napus and B. carinata with S. sclerotiorum, we examined the expression of five orthologs of B. napus genes involved in JA/ET or SA signaling pathways using quantitative RT-PCR. Our results indicated that there are differences in the timing of JA/ET and SA signaling pathways between B. napus and B. carinata. Our results in these two Brassica species also support previous observations that necrotrophic pathogens trigger JA/ET signaling in response to infection. Finally, we observed that transgenic canola expressing 1-aminocyclopropane-1-carboxylate-deaminase producing low levels of ET was relatively more susceptible to S. sclerotiorum than its wild-type counterpart, suggesting that ET inhibits S. sclerotiorum-induced symptom development.

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“…They act as systemic signals and can interact with other hormones and modulate production of transcription factors (TFs), protein kinases 3 as well as some phospholipases such as PLC. 4 The involvement of phospholipases in plant immunity to different stresses is due in part to early activation of signaling events. 5 Phospholipases have been shown to play important roles in resistance to various biotic and abiotic stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

et al. 2014

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Phosphatidylinositol-specific phospholipase C2 (PLC2) is a signaling enzyme with hydrolytic activity against membrane-bound phosphoinositides. It catalyzes the cleavage of phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5) P 2 ) into two initial second messengers, myo-inositol-1,4,5-trisphosphate (InsP 3 ) and diacylglycerol (DAG). The former, as well as its fully phosphorylated derivative, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP 6 ), play a major role in calcium signaling events within the cell, while DAG may be used in the regeneration of phospholipids or as a precursor for phosphatidic acid (PA) biosynthesis, an important signaling molecule involved in both biotic and abiotic types of stress tolerance. Overexpression of the gene for Brassica napus phospholipase C2 (BnPLC2) in Brassica napus has been shown to enhance drought tolerance, modulate multiple genes involved in different processes and favorably affect hormonal levels in different tissues. We, therefore, undertook the current study with a view to examining, at the metabolome level, its effect on both abiotic (low temperature) and biotic (stem white rot disease) types of stress in canola. Thus, while transgenic plants exhibited a significant rise in maltose levels and a concomitant elevation in some unsaturated free fatty acids (FFAs), glycerol, and glycerol 3-phosphate under subzero temperatures, they accumulated high levels of raffinose, stachyose and other sugars as well as some flavonoids under acclimatization conditions. Collectively, overexpression of BnPLC2 appears to have triggered different metabolite patterns consistent with its abiotic and, to a limited extent, biotic stress tolerance phenotypes.

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Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants

Cited by 46 publications

References 45 publications

Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions

Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions

Characterization of Defense Signaling Pathways of Brassica napus and Brassica carinata in Response to Sclerotinia sclerotiorum Challenge

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

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