Involvement of parathyroid hormone‐related protein in vascular calcification of chronic haemodialysis patients

Liu, Fang; Fu, Ping; Fan, Wenxing; Gou, Rong; Huang, Youqun; Qiu, Hui; Zhong, Hui; Huang, Songmin

doi:10.1111/j.1440-1797.2012.01601.x

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…Activation of BMP-2 signaling is associated with parathyroid hormone ligand, PTHLH, and its receptor, PTH1R. PreGC-IIc and oogonia overexpress PTHLH and PTH1R, respectively 59,60 .…”

Section: Crosstalk Between Germ and Supporting Cells Shapes Ovarian Architecturementioning

confidence: 99%

Single-cell roadmap of human gonadal development

Vento‐Tormo¹,

García-Alonso²,

Lorenzi³

et al. 2021

Preprint

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Gonadal development is a complex process that involves sex determination followed by divergent maturation into ovaries or testes. Historically, limited tissue accessibility and lack of reliable in vitro models have impeded our understanding of human gonadogenesis, despite its importance in gonadal pathologies and infertility. Here, we generated a comprehensive map of first- and second-trimester gonadal development using a combination of single-cell and spatial transcriptomics, chromatin accessibility assays and imaging. Using this approach, we identified novel transcription factors and cell states in human germ and supporting cell lineages. We compared them with other mammalian species and found primate-specific regulatory programmes. Our data identified cell context–specific interactions shaping sex specification and development of human germ cells. We defined a novel bipotent progenitor cell (LGR5+, TSPAN8+) in late embryos that can differentiate into early Sertoli in males or pre-granulosa cells in females. In fetal ovaries, we defined two subsets of pre-granulosa cells supporting germ-cell differentiation and distributed across the cortico-medullary axis. We also found a subset of developing granulosa cells appearing during the second trimester of pregnancy that is involved in follicular assembly. In fetal testes, we defined a novel supporting population (sPAX8 cells) located at the poles of the developing testis cords. We also found two tissue-resident myeloid populations that we named microglia-like and SIGLEC15+ fetal testicular macrophages. This study provides an unprecedented spatiotemporal map of human gonadal differentiation that can be utilised as a blueprint for in vitro gametogenesis.

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“…Activation of BMP-2 signaling is associated with parathyroid hormone ligand, PTHLH, and its receptor, PTH1R. PreGC-IIc and oogonia overexpress PTHLH and PTH1R, respectively 59,60 .…”

Section: Crosstalk Between Germ and Supporting Cells Shapes Ovarian Architecturementioning

confidence: 99%

Single-cell roadmap of human gonadal development

Vento‐Tormo¹,

García-Alonso²,

Lorenzi³

et al. 2021

Preprint

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“…The expression of the Runx2 is identified in arteries from CKD patients but not in normal vessels [21, 22], suggesting an important role of Runx2 in the pathogenesis of vascular calcification associated in CKD. Previous studies demonstrated that pooled uremic serum directly increased Runx2 expression and enhanced calcification of VSMC [22, 23]. Runx2 is regulated at multiple levels by a complex signal cascade.…”

Section: Regulation Of Vascular Calcification In Ckdmentioning

confidence: 99%

“…Using an ex vivo rat aortic ring assay, Lomashvili et al demonstrated that high calcium and/or high phosphate treatments induced medial VSMC calcification [42]. In vitro studies further demonstrated that high phosphate levels comparable to those seen in hyperphosphatemic individuals directly promoted osteogenic differentiation of VSMC, as indicated by increased expression of bone-related markers and loss of SMC marker genes [23, 43–45]. Similarly, extracellularly added calcium comparable to the levels observed in hypercalcemic subjects with or without addition of high phosphate induced VSMC calcification in vitro [45, 46•].…”

Section: Regulation Of Vascular Calcification In Ckdmentioning

confidence: 99%

Molecular Mechanisms of Vascular Calcification in Chronic Kidney Disease: The Link between Bone and the Vasculature

Byon

Chen

2015

Curr Osteoporos Rep

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Vascular calcification is highly prevalent in patients with chronic kidney disease (CKD) and increases mortality in those patients. Impaired calcium and phosphate homeostasis, increased oxidative stress, and loss of calcification inhibitors have been linked to vascular calcification in CKD. Additionally, impaired bone may perturb serum calcium/phosphate and their key regulator, parathyroid hormone, thus contributing to increased vascular calcification in CKD. Therapeutic approaches for CKD, such as phosphate binders and bisphosphonates, have been shown to ameliorate bone loss as well as vascular calcification. The precise mechanisms responsible for vascular calcification in CKD and the contribution of bone metabolism to vascular calcification have not been elucidated. This review discusses the role of systemic uremic factors and impaired bone metabolism in the pathogenesis of vascular calcification in CKD. The regulation of the key osteogenic transcription factor Runt-related transcription factor 2 (Runx2) and the emerging role of Runx2-dependent receptor activator of nuclear factor kappa-B ligand (RANKL) in vascular calcification of CKD are emphasized.

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“…The most common applications of Alizarin red staining are similar to those of Von Kossa, such as the recognition of calcification in coronary arteries and aorta, conditions strictly associated with increases in morbidity and mortality in end stage renal patients (112)(113).Moreover, the staining is also helpful for the development of possible therapeutic drugs able to suppress mineralization deposit in the vascular system (114). As many others stains, Alizarin red is not only useful for tissue sections but it can also be applied to plated cells, acquiring great importance in cell biology.…”

Section: Minerals Identificationmentioning

confidence: 99%

Dyes and Stains: from molecular structure to histological application

2014

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In the present review, the chemistry of dyes as well as the interaction mechanisms between tissue and dye has been detailed, and also some of the key factors affecting the selectivity of dyes by certain cellular structures have been mentioned. Moreover, due to the relevance that histological stains have acquired in biomedical research, some of the most common stains have been described, pointing out previous and current applications in basic and applied research.

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Involvement of parathyroid hormone‐related protein in vascular calcification of chronic haemodialysis patients

Abstract: Vascular calcification was common in patients with chronic haemodialysis, to which PTHrP might contribute by activating BMP-2/ Cbfa1 signalling pathway.

Cited by 10 publications

References 46 publications

Single-cell roadmap of human gonadal development

Single-cell roadmap of human gonadal development

Molecular Mechanisms of Vascular Calcification in Chronic Kidney Disease: The Link between Bone and the Vasculature

Dyes and Stains: from molecular structure to histological application

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