Involvement of PAF-Acether in Anaphylactic Bronchoconstriction Induced in Guinea Pigs by Aerosolized Antigen

Lagente, Vincent; Cirino, Maria; Desquand, S.; Lefort, Jean; Vargaftig, B.B.

doi:10.1159/000234469

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…These changes may, at least in the case of PAF, be due to the action of sulphidopeptide leukotrienes and thromboxanes, which have been shown to be released by PAF from non-mast cell populations in human lung tissue (Schleimer et al, 1986a). Furthermore, it has been demonstrated in the guinea-pig lung that repeated challenges with PAF desensitize against a subsequent allergen challenge but not vice versa (Lagente et al, 1988). This indicates that PAF activates other inflammatory cells that are not mast cells and do not respond to allergen.…”

Section: Discussionmentioning

confidence: 99%

Effect of nedocromil sodium on allergen‐, PAF‐, histamine‐ and bradykinin‐induced airways vasodilatation and pulmonary obstruction in the pig

Alving

Matran

Lundberg

1991

British J Pharmacology

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1 The influence of nedocromil sodium on the nasal and bronchial effects induced by allergen, plateletactivating factor (PAF), capsaicin, histamine and bradykinin aerosol challenge in ascaris-sensitized and pentobarbitone-anaesthetized pigs was studied. Blood flow changes in the bronchial and nasal circulation were measured with ultrasonic flow probes around the supplying arteries, and vascular resistance was calculated. Changes in pulmonary resistance (Rpul.), dynamic compliance (Cdy.), mean arterial pressure (MAP) and heart rate (HR) were also determined. 2 Allergen and PAF aerosol challenge in the lung produced similar effects consisting of both bronchial and nasal vasodilatation, bronchoconstriction (increase in Rpuim and decrease in CdY,) and increases in MAP and HR. Local pretreatment with nedocromil sodium (80mg, aerosol) reduced the peak and duration of both the bronchial vasodilatation and increase in Rpuim, while only the duration of the change in Cdyn was significantly decreased. Nedocromil sodium did not alter the increases in MAP and HR. The nasal vasodilatation evoked by PAF, but not allergen, challenge in the lung was reduced by nedocromil sodium. 3 Allergen challenge in the nose induced vasodilatation of long duration which was reduced by local nedocromil sodium pretreatment (50,pg kg-1, intra-arterially). 4 The vasodilator response to histamine aerosol was attenuated in the nasal, but not the bronchial circulation by local nedocromil sodium pretreatment. Histamine-induced bronchoconstriction was not altered by nedocromil sodium. 5 Bradykinin aerosol-induced vasodilatation in the nasal and bronchial circulation was markedly and equally reduced by local nedocromil sodium and systemic capsaicin (50mgkg-1, s.c. 2 days before) pretreatment. The vasodilatation caused by i.v. bradykinin challenge was not reduced by capsaicin pretreatment. Capsaicin aerosol-induced vasodilatation in the nasal and bronchial circulation was not affected by local nedocromil sodium pretreatment. 6 In conclusion, nedocromil sodium blocks some local vascular and bronchial effects, but not increases in MAP and HR, induced by allergen and PAF aerosol in the pig. Bradykinin-induced vasodilatation in the airways, which seems to be largely dependent on capsaicin-sensitive sensory nerves, is markedly inhibited by nedocromil sodium pretreatment, whereas capsaicin-induced vasodilatation is not affected by nedocromil sodium. It may be suggested that nedocromil sodium acts by inhibiting some common process involved in the release of mediators from inflammatory cells (when stimulated by allergen and PAF) and sensory nerves (when stimulated by bradykinin and histamine, but not capsaicin).

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Section: Discussionmentioning

confidence: 99%

Effect of nedocromil sodium on allergen‐, PAF‐, histamine‐ and bradykinin‐induced airways vasodilatation and pulmonary obstruction in the pig

Alving

Matran

Lundberg

1991

British J Pharmacology

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“…Pretolani et al [35] demonstrated that WEB 2086 antagon ized the pulmonary effects of paf-acether and ana phylactic shock in the guinea pig. In fact, a recent pu blication [36] demonstrated that passively sensitized animals which were made refractory to aerosolized paf-acether, also showed a reduced response to aeros olized ovalbumin. These data suggested that pafacether and antigen may interact in the lung.…”

Section: Discussionmentioning

confidence: 99%

Antigenic Contraction of Guinea Pig Tracheal Preparations Passively Sensitized with Monoclonal IgE: Pharmacological Modulation

Haye-Legrand¹,

Norel²,

Labat³

et al. 1988

Int Arch Allergy Immunol

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Spirally cut guinea pig tracheal preparations were passively sensitized using a mouse monoclonal IgE antibody against dinitrophenol (DNP). Maximal contraction observed following challenge with DNP-bovine serum albumin (DNP-BSA, 5 μg/ml; n = 20) response was approximately 46% of the histamine response (0.52 ± 0.09 g/mm²; n = 53). Indomethacin (1.7 μM) increased and PGE₂ (1 μM) decreased the response to the antigen. FPL 55712 (10 μM), atropine(0.1 μM), L 651392 (5 μM) or tripelennamine(1 μM) always reduced the maximal DNP-BSA response, but not BN 52021 (100 μM). This model may be used for rapid detection of compounds with antiallergic properties on IgE-dependent lung pathological states.

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“…Solutions of FMLP (10, 100 and 1000lgml-P during 2min), aspirin (300mgml-1 during 30min) and indomethacin (l00mgmlP1 during 20min) or the appropriate solvents, i.e., saline for FMLP (the stock solution of FMLP was prepared in DMSO at final concentration of 50 mg ml -1 and then diluted in saline to obtain 1000 ug ml -1) and aspirin, or NaOH (pH 7.8-8.0) for indomethacin (Boukili et al, 1986), were placed in the reservoir of a nebuliser connected to an air compressor, and the flow was directed into the outflow tube of the respiratory pump as described by Lagente et al (1988). FMLP was delivered 5 and 15 min after administration of aspirin and indomethacin intravenously or by aerosol, respectively.…”

Section: Guinea-pig In Vivo Preparationmentioning

confidence: 99%

Alveolar macrophages and eicosanoids but not neutrophils, mediate bronchoconstriction induced by FMLP in the guinea‐pig

Boukili

Bureau

Lellouch‐Tubiana

et al. 1989

British J Pharmacology

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1 N-formyl-methionyl-leucyl-phenylalanine (FMLP), when administered by aerosol to guineapigs, induced a dose-dependent bronchoconstriction (BC) with no overt effect on platelet and leukocyte blood counts. Repeated administration of FMLP by aerosol was followed by desensitization. 2 Electron microscopy studies showed that administration of FMLP by aerosol is accompanied by alveolar macrophage activation, accumulation and aggregation in the alveolar lumens. Nondegranulated eosinophils were observed in the lungs and a few platelet micro-aggregates in the pulmonary microvasculature.3 No significant accumulation of 13'I-labelled albumin, 1"'In-labelled neutrophils or "'Inlabelled platelets was detected in the lungs after the administration of FMLP by aerosol, whereas the intravenous administration was accompanied by an increase of extravascular albumin and significant neutrophil sequestration in the lungs. 4 Aspirin administered intravenously or by aerosol reduced significantly the BC induced by an aerosol of FMLP. By contrast, intravenous indomethacin reduced only BC induced by the submaximal dose of FMLP as an aerosol whereas, when administered by inhalation, it inhibited BC induced by FMLP administered either intravenously or by aerosol at all the concentrations tested. 5 FMLP induced a dose-dependent contraction of the guinea-pig trachea, which was not inhibited by indomethacin.

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Involvement of PAF-Acether in Anaphylactic Bronchoconstriction Induced in Guinea Pigs by Aerosolized Antigen

Cited by 5 publications

References 20 publications

Effect of nedocromil sodium on allergen‐, PAF‐, histamine‐ and bradykinin‐induced airways vasodilatation and pulmonary obstruction in the pig

Effect of nedocromil sodium on allergen‐, PAF‐, histamine‐ and bradykinin‐induced airways vasodilatation and pulmonary obstruction in the pig

Antigenic Contraction of Guinea Pig Tracheal Preparations Passively Sensitized with Monoclonal IgE: Pharmacological Modulation

Alveolar macrophages and eicosanoids but not neutrophils, mediate bronchoconstriction induced by FMLP in the guinea‐pig

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