Antigenic Contraction of Guinea Pig Tracheal Preparations Passively Sensitized with Monoclonal IgE: Pharmacological Modulation

Abstract

Spirally cut guinea pig tracheal preparations were passively sensitized using a mouse monoclonal IgE antibody against dinitrophenol (DNP). Maximal contraction observed following challenge with DNP-bovine serum albumin (DNP-BSA, 5 μg/ml; n = 20) response was approximately 46% of the histamine response (0.52 ± 0.09 g/mm²; n = 53). Indomethacin (1.7 μM) increased and PGE₂ (1 μM) decreased the response to the antigen. FPL 55712 (10 μM), atropine(0.1 μM), L 651392 (5 μM) or tripelennamine(1 μM) always reduced the maximal DNP-BSA response, but not BN 52021 (100 μM). This model may be used for rapid detection of compounds with antiallergic properties on IgE-dependent lung pathological states.