Involvement of oxidative stress in mutagenicity and apoptosis caused by dental resin monomers in cell cultures

Lee, Dong Hee; Lim, Bum-Soon; Lee, Yong‐Keun; Ahn, Sug-Joon; Yang, Hyeong‐Cheol

doi:10.1016/j.dental.2005.09.002

Cited by 109 publications

(79 citation statements)

References 29 publications

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“…Although ROS production is statistically insignificant following cotreatment of RES and UDMA or HEMA/Bis-GMA, there is a trend toward an increased ROS accumulation indicating that RES in combination with these agents may trigger oxidative stress. Our results are consistent with previous reports demonstrating that oxidative stress is directly involved in pathways leading to dentin bonding agents-induced cell death 4,33) and that RES addition to the formulations may exert protective effect.…”

Section: Discussionsupporting

confidence: 83%

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The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

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Section: Discussionsupporting

confidence: 83%

“…Resin-monomer induced toxicity results from oxidative stress and ROS production has a potential to induce DNA damage and mutagenity 33) . The increase of ROS generation in the cell renders all macromolecules such as lipids, proteins, carbohydrates and DNA becomes as a target in terms of oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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“…At present it is unclear how NAC protects the cells from cell death. However, previous work has attributed the protective effect of NAC in HEMA and TEGDMA mediated cell death to their anti-oxidant effect [18][19][20]. In contrast to these reports, our data suggests mechanisms for NAC function which are distinct from those shown previously and those mediated by other well established anti-oxidants such as Vitamin E and C [21].…”

Section: Introductioncontrasting

confidence: 96%

N-acetylcysteine protects dental pulp stromal cells from HEMA-induced apoptosis by inducing differentiation of the cells

Paranjpe

Cacalano

Hume

et al. 2007

Free Radical Biology and Medicine

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Resin based materials are now widely used in dental restorations. While the use of these materials is aesthetically appealing in patients, they carry the risk of local and systemic adverse effects. The potential risks are direct damage to the cells and induction of immune-based hypersensitivity reactions. Dental Pulp Stromal Cells (DPSCs) and oral keratinocytes are the major cell types which may come in contact with dental resins such as 2-hydroxyethyl methacrylate (HEMA) after dental restorations. Here we show that N-acetyl cysteine (NAC) inhibits HEMA induced apoptotic cell death and restores the function of DPSCs and oral epithelial cells. NAC inhibits HEMA mediated toxicity through induction of differentiation in DPSCs since the genes for dentin sialoprotein (DSP), Osteopontin (OPN), Osteocalcin (OCN), and Alkaline Phosphatase (ALP) which are induced during differentiation are also induced by NAC. Unlike NAC, Vitamin E and C which known anti-oxidant compounds failed to prevent either HEMA mediated cell death or decrease in VEGF secretion by human DPSCs. More importantly, when added either alone or in combination with HEMA Vitamin E and Vitamin C did not increase the gene expression for OPN, and in addition Vitamin E inhibited the protective effect of NAC on DPSCs. NAC inhibited HEMA mediated decrease in NFκB activity thus, providing survival mechanism for the cells. Overall, the studies reported in this paper indicated that undifferentiated DPSCs have exquisite sensitivity to HEMA induced cell death, and their differentiation by NAC resulted in an increased NFκB activity which might have provided the basis for their increased protection from HEMA mediated functional loss and cell death.

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“…[16][17][18] Rezin monomerlerde genel olarak sitotoksisite sıralaması Bis-GMA>UDMA>TEGDMA>HEMA şeklindedir. 16,19 Öte yandan moleküler ağırlık da difüzyon oranı nedeniyle monomerin sitotoksisitesinde etkili bir faktördür. HEMA, TEGDMA ve 4-metakroksiletil trimellitat anhidrit (4-META) gibi düşük molekül ağırlığına sahip monomerler Bis-GMA ve UDMA gibi daha visköz rezinler için solvent işlevi görmekte ve bunların hücre ve dokulara daha çok difüze olmasına neden olmaktadır.…”

Section: Biyouyumluluğun Değerlendirilmesiunclassified

“…[61][62][63] ROS artışının hücrenin lipit, protein ve karbonhidrat gibi yapısal bileşenlerini ve DNA'yı oksidatif hasara uğratması ve apoptozise neden olması mümkündür. 8,19,64 Oksidatif stres her zaman mutajenik koşullar oluşturmayabilir, ancak hücre içi oksidatif stres ve ROS üretimine neden olan kimyasallar mutajenite için de potansiyel oluşturmaktadır. 65 Materyalin pulpa dokusu ve hücrelerinde oluşturacağı biyolojik etkide, serbest monomerleri absorbe ederek bir tür bariyer görevi yapan dentin dokusu önemli bir faktördür.…”

Section: Dolayısıylaunclassified

An Overview to the Cytotoxicity of Resin Based Dental Materials

Atalayın¹,

Tezel²,

Ergücü³

2016

EÜ Dişhek Fak Derg

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ÖZETDişhekimliği pratiğinde rezin esaslı materyaller estetik özellikleri nedeni ile ön plana çıkmaktadır. Günümüzde klinik başarı sadece materyalin mekanik ve estetik özellikleri değil, aynı zamanda biyolojik açıdan güvenirliliği ve dokularla uyumluluğu ile de ilişkilendirilmektedir. Bu yaklaşımdan hareketle rezin esaslı dental materyallerin içeriğindeki monomerler nedeni ile biyouyumlulukları sorgulanmaktadır. Bu derlememin amacı biyoyumluluğa ilişkin temel kavramları ve yöntemleri gözden geçirmek, rezin esaslı materyallerin sitotoksisitesine ilişkin çalışmaların verilerini sunmak ve son olarak klinik uygulamalara ilişkin tavsiyeleri bildirmektir. Anahtar Kelimeler: biyouyumluluk, dental materyal, rezin, monomer, sitotoksisite ABSTRACT

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Involvement of oxidative stress in mutagenicity and apoptosis caused by dental resin monomers in cell cultures

Cited by 109 publications

References 29 publications

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

N-acetylcysteine protects dental pulp stromal cells from HEMA-induced apoptosis by inducing differentiation of the cells

An Overview to the Cytotoxicity of Resin Based Dental Materials

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