Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress

Bustos, Diego Martín; Bustamante, Claudia A.; Iglesias, Alberto A.

doi:10.1016/j.jplph.2007.06.005

Cited by 38 publications

(31 citation statements)

References 20 publications

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“…Remarkably, organisms that do not depend on the degradation of organic compounds for energy, such as phototrophs, may forfeit glycolytic ATP production altogether. These organisms substantially increase the chemical motive force of the pathway reactions by bypassing the substrate-level phosphorylation step and using glycolysis solely for the production of carbon skeletons 90,91 .…”

Section: The Physicochemical Properties Of Intermediatesmentioning

confidence: 99%

Rethinking glycolysis: on the biochemical logic of metabolic pathways

et al. 2012

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Metabolic pathways may seem arbitrary and unnecessarily complex. In many cases, a chemist might devise a simpler route for the biochemical transformation, so why has nature chosen such complex solutions? In this review, we distill lessons from a century of metabolic research and introduce new observations suggesting that the intricate structure of metabolic pathways can be explained by a small set of biochemical principles. Using glycolysis as an example, we demonstrate how three key biochemical constraints--thermodynamic favorability, availability of enzymatic mechanisms and the physicochemical properties of pathway intermediates--eliminate otherwise plausible metabolic strategies. Considering these constraints, glycolysis contains no unnecessary steps and represents one of the very few pathway structures that meet cellular demands. The analysis presented here can be applied to metabolic engineering efforts for the rational design of pathways that produce a desired product while satisfying biochemical constraints.

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Section: The Physicochemical Properties Of Intermediatesmentioning

confidence: 99%

Rethinking glycolysis: on the biochemical logic of metabolic pathways

et al. 2012

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“…In Arabidopsis, for example, it has been shown that GAPDH proteolysis is modulated by binding to regulatory 14-3-3 proteins, through protein phosphorylation [33]. In plants, a cytosolic nonphosphorylating GAPDH is occurring in addition to glycolitic GAPDH; this phosphorylated enzyme is involved in the generation of NADPH and is modulated by oxidative stress [34]. Proteins belonging to the photosynthetic apparatus, namely two components of the photosystem II oxygenevolving complex (spots 4 and 5), a component of the cytochrome b 6 -f complex (spot 9) and an extrinsic protein associated to the PS II complex (spot 7), also showed lower levels of their phosphorylated species.…”

Section: Chitosan Treatmentmentioning

confidence: 99%

Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

Huang

Verrillo

Renzone

et al. 2011

Journal of Proteomics

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“…Metabolic flux has to be regulated between generating hexose-Ps via gluconeogenesis and glycolytic "downstream" products, such as 3-PGA, PEP, and pyruvic acid. In higher plants, the redox transfer by a TP/3-PGA shuttle via nonphosphorylating glyceraldehyde-3-P dehydrogenase and 3-PGA kinase is proposed to be marginal and glyceraldehyde-3-P dehydrogenase expression is mainly induced under stress conditions, such as anaerobiosis and heat shock or Suc feeding (Yang et al, 1993;Bustos et al, 2008;Holtgrefe et al, 2008). On the other hand, glycolysis In photosynthetically active cells, TP is exported via the GsTPT from rhodoplasts to sustain soluble and insoluble carbohydrate synthesis (floridoside and floridean starch) in the cytosol.…”

Section: Impact Of Subtle Differences Of Tpt In Chloroplastida and Rhmentioning

confidence: 99%

Functional Characterization of the Plastidic Phosphate Translocator Gene Family from the Thermo-Acidophilic Red Alga Galdieria sulphuraria Reveals Specific Adaptations of Primary Carbon Partitioning in Green Plants and Red Algae

2008

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In chloroplasts of green plants and algae, CO 2 is assimilated into triose-phosphates (TPs); a large part of these TPs is exported to the cytosol by a TP/phosphate translocator (TPT), whereas some is stored in the plastid as starch. Plastidial phosphate translocators have evolved from transport proteins of the host endomembrane system shortly after the origin of chloroplasts by endosymbiosis. The red microalga Galdieria sulphuraria shares three conserved putative orthologous transport proteins with the distantly related seed plants and green algae. However, red algae, in contrast to green plants, store starch in their cytosol, not inside plastids. Hence, due to the lack of a plastidic starch pool, a larger share of recently assimilated CO 2 needs to be exported to the cytosol. We thus hypothesized that red algal transporters have distinct substrate specificity in comparison to their green orthologs. This hypothesis was tested by expression of the red algal genes in yeast (Saccharomyces cerevisiae) and assessment of their substrate specificities and kinetic constants. Indeed, two of the three red algal phosphate translocator candidate orthologs have clearly distinct substrate specificities when compared to their green homologs. GsTPT (for G. sulphuraria TPT) displays very narrow substrate specificity and high affinity; in contrast to green plant TPTs, 3-phosphoglyceric acid is poorly transported and thus not able to serve as a TP/3-phosphoglyceric acid redox shuttle in vivo. Apparently, the specific features of red algal primary carbon metabolism promoted the evolution of a highly efficient export system with high affinities for its substrates. The low-affinity TPT of plants maintains TP levels sufficient for starch biosynthesis inside of chloroplasts, whereas the red algal TPT is optimized for efficient export of TP from the chloroplast.

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Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress

Cited by 38 publications

References 20 publications

Rethinking glycolysis: on the biochemical logic of metabolic pathways

Rethinking glycolysis: on the biochemical logic of metabolic pathways

Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

Functional Characterization of the Plastidic Phosphate Translocator Gene Family from the Thermo-Acidophilic Red Alga Galdieria sulphuraria Reveals Specific Adaptations of Primary Carbon Partitioning in Green Plants and Red Algae

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