Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

Huang, Chao; Verrillo, Francesca; Renzone, Giovanni; Arena, Simona; Rocco, Mariapina; Scaloni, Andrea; Marra, Mauro

doi:10.1016/j.jprot.2011.05.016

Cited by 37 publications

(33 citation statements)

References 87 publications

(102 reference statements)

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“…Our research also suggests a role for their differential phosphorylation status in response to biotic stress. These findings are in good agreement with recent reports suggesting that mechanisms by which plants respond to different stresses (abiotic and biotic) are not independent, but instead participate in crosstalk with one another and share several biochemical networks at least partially overlapped (Cerny et al 2011;Huang et al 2011;Atkinson and Urwin 2012).…”

Section: Discussionsupporting

confidence: 93%

Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus

Wang

et al. 2014

Amino Acids

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Protein phosphorylation plays a pivotal role in the regulation of many cellular events. No information is yet available, however, on protein phosphorylation in plants in response to virus infection. In this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (Zea mays L.) in response to Sugarcane mosaic virus (SCMV) infection. Based on isotope tags for relative and absolute quantification technology, TiO2 enrichment method and LC-MS/MS analysis, we identified 65 and 59 phosphoproteins respectively, whose phosphorylation level regulated significantly in susceptible and resistant plants. Some identified phosphoproteins were shared by both genotypes, suggesting a partial overlapping of the responsive pathways to virus infection. While several phosphoproteins are well-known pathogen response phosphoproteins, virus infection differentially regulates most other phosphoproteins, which has not been reported in literature. Changes in protein phosphorylation status indicated that response to SCMV infection encompass a reformatting of major cellular processes. Our data provide new valuable insights into plant-virus interactions.

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Section: Discussionsupporting

confidence: 93%

Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus

Wang

et al. 2014

Amino Acids

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“…In parallel, a bi-dimensional electrophoresis analysis of protein extracts was used to identify specific proteomic changes in drupes with larval feeding tunnels. Gel-based proteomic studies have been extensively used to investigate protein expression changes in plant tissues during responses to biotic or abiotic stress and to highlight molecular signatures in genotypes with higher levels of resistance to insects or fungi [20-24]. Our transcriptomic and proteomic analyses allowed us to reveal the molecular bases and related signalling pathways induced in the interaction between olive and its most damaging biotic pest.…”

Section: Introductionmentioning

confidence: 99%

Molecular interactions between the olive and the fruit fly Bactrocera oleae

et al. 2012

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BackgroundThe fruit fly Bactrocera oleae is the primary biotic stressor of cultivated olives, causing direct and indirect damages that significantly reduce both the yield and the quality of olive oil. To study the olive-B. oleae interaction, we conducted transcriptomic and proteomic investigations of the molecular response of the drupe. The identifications of genes and proteins involved in the fruit response were performed using a Suppression Subtractive Hybridisation technique and a combined bi-dimensional electrophoresis/nanoLC-ESI-LIT-MS/MS approach, respectively.ResultsWe identified 196 ESTs and 26 protein spots as differentially expressed in olives with larval feeding tunnels. A bioinformatic analysis of the identified non-redundant EST and protein collection indicated that different molecular processes were affected, such as stress response, phytohormone signalling, transcriptional control and primary metabolism, and that a considerable proportion of the ESTs could not be classified. The altered expression of 20 transcripts was also analysed by real-time PCR, and the most striking differences were further confirmed in the fruit of a different olive variety. We also cloned the full-length coding sequences of two genes, Oe-chitinase I and Oe-PR27, and showed that these are wound-inducible genes and activated by B. oleae punctures.ConclusionsThis study represents the first report that reveals the molecular players and signalling pathways involved in the interaction between the olive fruit and its most damaging biotic stressor. Drupe response is complex, involving genes and proteins involved in photosynthesis as well as in the production of ROS, the activation of different stress response pathways and the production of compounds involved in direct defence against phytophagous larvae. Among the latter, trypsin inhibitors should play a major role in drupe resistance reaction.

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“…Proteins were extracted according to the phenol-based extraction method for Arabidopsis plants of Huang et al (2011), with some modifications. Independent, frozen Arabidopsis leaves (0.3-0.4 g fresh mass) were finely powdered in liquid nitrogen using a pestle and mortar, and suspended in 2.5-volumes of extraction buffer (0.9 M sucrose, 0.5 M Tris, 0.005 M EDTA, 0.1 M KCl, and 1% W/V DTT) by vortexing until obtaining a thick paste.…”

Section: Protein Extractionmentioning

confidence: 99%

Plant defense in response to chewing insects: proteome analysis ofArabidopsis thalianadamaged byPlutella xylostella

Truong

Nguyen

Bauwens

et al. 2017

Journal of Plant Interactions

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The interactions between Arabidopsis thaliana and Plutella xylostella have been considered as a model system to unravel the responses of plants to herbivorous insects. Here, we use a 2-DE proteome approach to detect protein expression changes in the leaves of Arabidopsis plants exposed to P. xylostella larval infestation at 27°C within 8 h. Approximately 450 protein spots were reproducibly detected on gels. Of these, comparing healthy and infested leaves, we identified 18 differentially expressed protein spots. Thirteen proteins were successfully identified by MALDI-TOF/MS and LC-ESI-MS/MS. Functional classification analysis indicated that the differentially identified proteins were associated with amino acid, carbohydrate, energy, lipid metabolism, and photosynthesis. In addition, their relative abundances were assessed according to larval pest feeding on Arabidopsis leaves. These data provide valuable new insights for further works in plant-biotic and environmental stress interaction. ARTICLE HISTORY

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Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

Cited by 37 publications

References 87 publications

Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus

Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus

Molecular interactions between the olive and the fruit fly Bactrocera oleae

Plant defense in response to chewing insects: proteome analysis ofArabidopsis thalianadamaged byPlutella xylostella

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