Involvement of free and conjugated polyamines and free amino acids in the adventitious rooting of micropropagated cork oak and grapevine shoots

Neves, Clélia; Santos, Helena; Boas, L. F. Vilas; Amâncio, Sara

doi:10.1016/s0981-9428(02)01472-9

Cited by 30 publications

(15 citation statements)

References 31 publications

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“…Similar polyamine effect on root formation of apple rootstock microcuttings was reported by Naija et al [2009]. In turn, the high positive correlation between in vitro rooting and the increased level of endogenous free polyamines, particularly Put was reported by Neves et al [2002]. Moreover, these authors concluded that free Put can be considered as a marker of in vitro root induction phase.…”

Section: Discussionsupporting

confidence: 74%

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ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

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For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid -IBA, 1-naphthaleneacetic acid -NAA and indole--3-acetic acid -IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars 'Free Redstar', 'Gala Must', 'Pinova' and 'Redchief' were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60-80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.

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Section: Discussionsupporting

confidence: 74%

“…The positive impact of arginine on rooting, observed in our and other studies may indicate major role for arginine decarboxylation rather than ornithine in Put synthesis. It was suggested that such a mechanism of Put biosynthesis with arginine decarboxylation could function in Phaseolus [Palavan-Ünsal 1987] and cork oat [Neves et al 2002].…”

Section: Discussionmentioning

confidence: 99%

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

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“…Also, the results from the application of exogenous polyamines suggest that endogenous concentration of these polyamines could regulate growth and development in higher plants (Tang and Newton 2005;Martinez-Pastur et al 2007). A correlation between polyamine accumulation and the initial stages of adventitious root formation has been observed by several authors (Jarvis et al 1985;Biondi et al 1990;Heloir et al 1996;Neves et al 2002), suggesting that polyamines could also be used as markers of the rooting process.…”

Section: Introductionmentioning

confidence: 88%

Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106

Naija

Elloumi²,

Ammar

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Apple rootstock MM106 shoots, raised in vitro, rooted at 96.7% after culture on a medium supplemented with an auxin for 5 d in darkness followed by culture on a second medium without growth regulators for 25 d in light. In control conditions (in absence of auxin in the first medium), these shoots did not root. Putrescine (PUT), spermidine (SPD), cyclohexylamine (CHA), and aminoguanidine (AG) enhanced rooting when applied during the first d of culture in the absence of IBA; on the contrary, α-difluoromethylornithine (DFMO) added to the first medium with IBA inhibited rooting. The endogenous levels of indole 3-acetic acid (IAA) and indole 3-acetylaspartic acid (IAAsp) increased up to a maximum concentration at days 2 and 3, respectively, in initial rooting conditions. PUT, when added with IBA, did not affect the typical IAA and IAAsp increase; when applied alone, it provoked an increase of their levels. Similar results were recorded with CHA. SPD, AG, and DFMO did not induce an increase of IAA and IAAsp in nonrooting conditions. The levels of endogenous PUT increased to a maximum at day 2 in rooting conditions; it was slightly affected by exogenous PUT and CHA application but reduced by SPD, AG, and DFMO. In rooting conditions, if the first medium was supplemented with SPD or AG, a small increase in peroxidase activity was observed, similar to that obtained with PUT treatment. The present work indicates an involvement of polyamines in the control of rooting and an interaction with auxins during the physiological phase of rooting. The consequence of this relationship was a different rooting expression, according especially to the content of these regulators in the culture medium.

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“…Polyamines bound to low molecular mass compounds, such as hydroxycinnamic acids are designated as conjugated polyamines, which are soluble in perchloric acid (PCA). Polyamines bound to high molecular mass compounds, such as DNA, proteins, nucleic acids, and membrane phospholipids are designated as bound polyamines, which are insoluble in PCA (Neves et al 2002). Also, conjugated and bound polyamines are associated with plant tolerance to stresses.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ca(NO3)2 stress on oxidative damage, antioxidant enzymes activities and polyamine contents in roots of grafted and non-grafted tomato plants

et al. 2008

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The effects of Ca(NO 3 ) 2 stress on biomass production, oxidative damage, antioxidant enzymes activities and polyamine contents in roots of grafted and non-grafted tomato plants were investigated. Results showed that when exposed to 80 mM Ca(NO 3 ) 2 stress, the biomass production reduction in non-grafted plants was more significant than that of grafted plants. Under Ca(NO 3 ) 2 stress, superoxide anion radical (O 2 • -) producing rate, hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) contents of non-grafted plants roots were significantly higher than those of grafted plants, however, nitrate (NO 3 -), ammonium (NH 4

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Involvement of free and conjugated polyamines and free amino acids in the adventitious rooting of micropropagated cork oak and grapevine shoots

Cited by 30 publications

References 31 publications

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106

Effects of Ca(NO3)2 stress on oxidative damage, antioxidant enzymes activities and polyamine contents in roots of grafted and non-grafted tomato plants

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