Effects of Ca(NO3)2 stress on oxidative damage, antioxidant enzymes activities and polyamine contents in roots of grafted and non-grafted tomato plants

Abstract

The effects of Ca(NO 3 ) 2 stress on biomass production, oxidative damage, antioxidant enzymes activities and polyamine contents in roots of grafted and non-grafted tomato plants were investigated. Results showed that when exposed to 80 mM Ca(NO 3 ) 2 stress, the biomass production reduction in non-grafted plants was more significant than that of grafted plants. Under Ca(NO 3 ) 2 stress, superoxide anion radical (O 2 • -) producing rate, hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) contents of non-grafted plants roots were significantly higher than those of grafted plants, however, nitrate (NO 3 -), ammonium (NH 4

Effects of salt-tolerant rootstock grafting on ultrastructure, photosynthetic capacity, and H2O2-scavenging system in chloroplasts of cucumber seedlings under NaCl stress

“…This is in accordance with the findings in watermelon (Colla et al 2006;Goreta et al 2008), tomato (Martinez-Rodriguez et al 2008Zhang et al 2008), and our previous reports in cucumber Zhen et al 2010;Zhu et al 2008). Growth inhibition induced by salinity is often associated with a decrease in their photosynthetic capacity (Zuccarini 2008;He et al 2009).…”

“…Rootstock grafting seems to enhance the antioxidant enzyme activities involved in the ascorbate-glutathione system, and then controls the H 2 O 2 concentration in the chloroplasts of cucumber leaves under NaCl stress to avoid oxidative damage at subcellular levels. This result is generally consistent with those found in the leaves of watermelon (Colla et al 2006;Goreta et al 2008), tomato (He et al 2009;Zhang et al 2008), and cucumber . The serious structural alteration of chloroplasts in self-grafted plant leaves is also likely caused by H 2 O 2 oxidative damage, because H 2 O 2 , a more stable ROS, can diffuse across biological membranes and cause oxidative protein modifications in areas distant from its production (Asada 2006).…”

“…He et al (2009) concluded that the alleviation of growth inhibition of rootstock-grafted plants by salt stress may be related to improvement of photosynthesis and enhancement of antioxidant enzymes activity. Although grafting has been reported to be capable of enhancing plant antioxidant levels under salt stress (He et al 2009;Wei et al 2009;Zhang et al 2008;Zhen et al 2010), its roles in promoting tolerance at the chloroplast level has not yet been studied. Furthermore, although grafting with salt-tolerant rootstock plays an important role in improving the photosynthetic performance of plants under salinity (He et al 2009;Massai et al 2004), data on the effects of grafting on the chloroplast ultrastructure in plants exposed to salinity was limited.…”

Regulation of 2,4-epibrassinolide on mineral nutrient uptake and ion distribution in Ca(NO3)2 stressed cucumber plants

“…1 Equal contributions to this paper. Zhang et al, 2008). However, there is little research on ion damage by excessive Ca(NO 3 ) 2 .…”

Calcium Salts Enhance Activity and Azo Dye Decolourisation Capacity of Crude Peroxidase from <i>Armoracia rusticana</i>

“…This suggests that the higher concentration of nitrate ions ( ) 3 NO − in the Ca(NO 3 ) 2 treated set may have contributed to an additional increase in peroxidase activity in these roots. The peroxidase activity in roots of tomato plants treated with Ca(NO 3 ) 2 has been reported to be significantly higher than in roots of untreated plants [15]. Similarly, the peroxidase activity in the roots of transgenic tobacco plants was found to increase with an increasing concentration of 3 …”