Involvement of ethylene in somatic embryogenesis in Scots pine (Pinus sylvestris L.)

Lu, Jinrong; Vahala, Jorma; Pappinen, Ari

doi:10.1007/s11240-011-9952-4

Cited by 37 publications

(21 citation statements)

References 48 publications

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“…Huxter et al (1981). Ethylene production has also been described as influencing the development of somatic embryos in several works that have reported regeneration through the embryogenic pathway (Ptak et al 2009;Lu et al 2011). …”

Section: Effect On Organogenesis Of Different Doses Of Ethylene Inhibmentioning

confidence: 99%

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The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

Trujillo-Moya

Gisbert

2012

Plant Cell Tiss Organ Cult

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Abstract:The influence of ethylene and ethylene modulators on the in vitro organogenesis of tomato was studied using a highly regenerating accession of the wild tomato Solanum pennellii and an F1 plant resulting from a cross between Solanum pennellii and Solanum lycopersicum cv. Anl27, which is known to have a low regeneration frequency. Four ethylene-modulating compounds, each at four levels, were used, namely: cobalt chloride (CoCl2), which inhibits the production of ethylene; AgNO3 (SN), which inhibits ethylene action; and Ethephon and the precursor 1-aminocyclopropane-1-carboxylic acid (ACC), which both promote ethylene synthesis. Leaf explants of each genotype were incubated on shoot induction medium (SIM) supplemented with each of these compounds at 0, 10 or 15 days following bud induction. The results obtained in our assays indicate that ethylene has a significant influence on tomato organogenesis. Concentrations of ethylene lower than the optimum (according to genotype) at the beginning of the culture may decrease the percentage of explants with buds (B), produce a delay in their appearance, or indeed inhibit bud formation. This was observed in S. pennellii and the F1 explants cultured on media with SN (5.8-58.0 µM) as well as in the F1 explants cultured on medium with 21.0 µM CoCl2. The percentage of explants with shoots (R) and the mean number of shoots per explant with shoots (PR) also diminished in media that contained SN. Shoots isolated from these explants were less developed compared to those isolated from control explants. On the other hand, ethylene supplementation may contribute to enhancing shoot development. The number of isolable shoots from S. pennellii explants doubled in media with ACC (9.8-98.0 µM). Shoots isolated from explants treated with ethylene releasing compounds showed a higher number of nodes when ACC and Ethephon were added at 10 days (in F1 explants) or at 15 days (in S. pennellii) after the beginning of culture. Thus, the importance of studying not only the concentration but also the timing of the application of regulators when developing regeneration protocols has been made manifest. An excess of ethylene supplementation may produce an inhibitory effect, as was observed when using Ethephon (17.2-69.0 µM). These results show the involvement of ethylene in tomato organogenesis and lead us to believe that ethylene supplementation may contribute to enhancing regeneration and shoot development in tomato.Response to Reviewers: Dear Dr. Schuyler, Attached please find the revised version of our manuscript TICU5251, "The role of Powered by Editorial Manager® and Preprint Manager® from Aries Systems Corporationethylene in tomato shoot regeneration," which we would like to publish in Plant Cell, Tissue and Organ Culture. The revised version has been prepared taking into account your comments and those of the reviewers. Below, we have provided a detailed list of the changes made in response to the reviewers' comments. Looking forward to hearing from you. Best Regards, Carmina GisbertAns...

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Section: Effect On Organogenesis Of Different Doses Of Ethylene Inhibmentioning

confidence: 99%

“…Please inform of any further changes deemed necessary. 4.We have included three articles published in PCTOC, Dias et al (2009), Lu et al (2011) and Ptak et al (2010), in addition to two that were cited in the previous version (Bhatia et al 2004 andMohiudding et al 1997).…”

mentioning

confidence: 99%

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

Trujillo-Moya

Gisbert

2012

Plant Cell Tiss Organ Cult

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“…However, ACS and ACO expression levels were greater in embryogenic tissue and somatic embryos than in a non-embryogenic callus in M. truncatula, and the genes were expressed at an early growth stage as well as throughout the culture period (Mantiri et al, 2008). ACS members showed differential upregulation during the proliferation and maturation of somatic embryos from a callus of Pinus sylvestris (Lu et al, 2011). Timing of chemical treatment was critical for ethylene production and proper application of ethylene inhibitors or precursors at an appropriate physiological stage of barley and M. sativa L. explants affected the regeneration capability (Tyagi and Dahleen, 2011).…”

Section: Introductionmentioning

confidence: 94%

Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

et al. 2014

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ABSTRACT. Phalaenopsis orchids have been regenerated by inducing protocorm-like bodies (PLBs) from etiolated leaf sections. However, the physiological and molecular mechanisms of secondary PLB development and subsequent proliferation have not been explored. Bisectionally cutting primary PLBs resulted in more secondary PLBs at 5 weeks, suggesting an embryogenic stem cell property imposed by wounding of primary PLB tissues. The ethylene precursors ethephon and 1-aminocyclopropanecarboxylic acid and the ethylene perception inhibitor silver nitrate increased PLB formation, while aminoethoxyvinylglycine decreased PLB formation. Ethylene content in wounded PLB explants increased over culture time in media containing ethylene precursors or inhibitors. mRNA levels of PhACS2, PhACS3, and PhACO were increased by ethephon and decreased by ethylene inhibitors. Expression of genes in the ethylene signaling pathway was enhanced following ethylene-precursor treatment and was mitigated by ethylene inhibitors during PLB proliferation. Transcription of PhETR and PhEIN3, as well as PhERS, PhCTR, and PhGTP, was significantly increased 12 h after ethylene treatment. Ethylene and physical wounding stimulated secondary PLB formation in Phalaenopsis, probably through ethylene biosynthesis and signal transduction.

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“…[64], Medicago sativa [65], Pinus sylvestris [66], and Quercus ilex [67]. However, in some other plant species such as black spruce [68] and Leucojum aestivum [69] ethylene behaves negatively during somatic embryo development.…”

Section: Ethylene-inducible Genesmentioning

confidence: 99%

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

2018

J App Biol Biotech

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Based on the previous available documents involving molecular events during plant somatic embryogenesis, this report aims to review the advances that have been made for the past several years in the area of molecular mechanism of plant somatic embryogenesis. To begin with, studies suggest that the induction and differentiation of embryos from somatic tissue directly or through callusing involves the interaction of various cellular and molecular factors. Several intra-and extra-cellular proteins such as germins and germins-like proteins, lipid transfer proteins, heatshock proteins, and late embryogenesis abundant proteins are known to regulate the induction of somatic embryos from the somatic cell. Simultaneously, regulation and expression of specific genes such as housekeeping genes OsIAA in rice; hormone-responsive genes Dcarg-1, Dchsp-1, DcECP31, DcEMB1 in carrot; and AtECP63, Mt somatic embryo-related factor 1 in arabidopsis have been identified to play key roles during the process of somatic embryogenesis. These genes are known to express differentially for synthesis of new proteins during induction and development of somatic embryo. In addition, several transcription factors such as leafy cotyledon genes, agamouslike15 (AGL15) gene, ethylene-responsive element-binding protein (EREBPs), knotted1-like homeobox proteins, and RWP-RK group of plant-specific transcription factors are equally known that efficiently control the molecular events of somatic embryogenesis. Further, it is also now established that epigenetic factors such asDNA methylation, histone deacetylation/methylation, and microRNAs also influence the molecular mechanism of plant somatic embryogenesis.

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Involvement of ethylene in somatic embryogenesis in Scots pine (Pinus sylvestris L.)

Cited by 37 publications

References 48 publications

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

The influence of ethylene and ethylene modulators on shoot organogenesis in tomato

Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

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