Involvement of bone morphogenetic protein–related pathways in the effect of aucubin on the promotion of osteoblast differentiation in MG63 cells

Li, Yutong; Hu, Wenjing; Han, Guanghong; Lü, Wei; Jia, Dongxu; Hu, Min; Wang, Di

doi:10.1016/j.cbi.2018.02.005

Cited by 29 publications

(22 citation statements)

References 58 publications

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“…The expression level of C-C chemokine receptor type 5 (CCR5) and chemokine receptor type 4 (CXCR4) on CD4+ Tcellswere decreased in cells treated with this iridoid glycoside, demonstrated that this iridoid glycoside restricts HIV-1 replication on the early stage of HIV infection [35] Antiproliferative activities Deacetyl asperuloside 806.4 µg/mL K562 chronic myelogenous leukemic cells Significantly increased caspase 3 activity (p < 0.05) [16] AChE inhibitory Lupulinoside 134.0 µM GST, AChE Lupulinoside exhibited different levels of GST, AChE inhibitory [41] Anti-obesity Genipin 20 mg/kg High-fat diet-fed obese mice Regulating miR-142a-5p/SREBP-1c axis, led to the inhibition of lipogenesis [96] Anti-osteoporosis activity Aucubin 1, 2.5, 5 µM MG63 cells Improved osteoblast differentiation and enhanced the levels of BMP2 (bone morphogenetic proteins-2) in MG63 cells [97] Nuezhenelenoliciside -MC3T3-E1 cells Increased the proliferation of pre-osteoblast MC3T3-E1 cells, possessed anti-osteoporosis activity [11]…”

Section: Othersmentioning

confidence: 99%

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

et al. 2020

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Iridoids are a class of active compounds that widely exist in the plant kingdom. In recent years, with advances in phytochemical research, many compounds with novel structure and outstanding activity have been identified. Iridoid compounds have been confirmed to mainly exist as the prototype and aglycone and Ι and II metabolites, by biological transformation. These metabolites have been shown to have neuroprotective, hepatoprotective, anti-inflammatory, antitumor, hypoglycemic, and hypolipidemic activities. This review summarizes the new structures and activities of iridoids identified locally and globally, and explains their pharmacokinetics from the aspects of absorption, distribution, metabolism, and excretion according to the differences in their structures, thus providing a theoretical basis for further rational development and utilization of iridoids and their metabolites.

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Section: Othersmentioning

confidence: 99%

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

et al. 2020

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“…Bone Morphogenetic Proteins (BMPs) are in the transforming growth factor-β (TGFβ) superfamily, which is important in the regulation of bone induction, maintenance and repair [25]. BMP-2 has been considered to be the most active osteogenesis factor in TGF-β superfamily.…”

Section: Discussionmentioning

confidence: 99%

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

Gou

et al. 2018

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Icariin, the main active ingredient of Epimedium, has played an important role in bone anabolism. However, the molecular mechanism for this effect was not convincingly reported yet. In this paper, the role of icariin on cell morphology, viability, cell cycling and the activity of alkaline phosphatase (ALP) were studied, and the molecular mechanism of icariin induced osteogenic effect was also investigated. Icariin with different concentrations (10, 20 and 40 ng/ml) was used to modify the pre-osteoblastic MC3T3-E1 cells for 48, 72 and 96 h, and the proliferation, morphology, and the cell cycle of the cells were evaluated by Cell Counting Kit-8 (CCK-8), microscopy and flow cytometry, respectively. Bone morphogenic protein-2 (BMP-2), bone morphogenic protein receptor-2 (BMPR-2), Smad4, Smadl/5/8 proteins expression levels were obtained by Western blotting and the expression levels of runt-related transcription factor 2 (Runx2) mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR). In this study, we found that icariin could promote the proliferation and differentiation of MC3T3-E1 cells in a dose-and time-dependent manner. Icariin could stimulate the expression of the BMP-2, BMPR-2, Smad4 and Smadl/5/8 proteins.Furthermore, icariin could upregulate the expression of Runx2 mRNA. These results showed that icariin played an important role in upregulating BMP-2 expression to activate the BMP-2/Smads/Runx2 signal pathway for increasing both the proliferation and differentiation of the MC3T3-E1 cells. However, the osteogenic effects of icariin can be suppressed by the BMP-2 antagonist (Noggin). In conclusion, we demonstrate that icariin is an osteoinductive factor that exerts its osteogenic effect by regulating the BMP-2/Smads/Runx2 signal pathway in MC3T3-E1 cells.

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“…It is a positive effect for hydroxyl-carbonate-apatite formation, but it makes the pH-dependent cytotoxicity become a significant issue to evaluate the bioactivity of materials [15]. Therefore, the human osteosarcoma cells (MG63, ATCC ® CRL-1427TM) were used in this study to evaluate cell viability [16]. Dulbecco's modified Eagle medium (DMEM) was used for MG63 cells.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Properties for Bioceramics Composed of Silica and Titanium Oxide Composites

et al. 2018

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It is important for oral and maxillofacial surgeons to repair craniofacial defects on oral cancer patients or patients with congenital problems. Thus, it is a challenge to develop biomaterials that promote bone regeneration as potential materials for bone repair. This work is devoted to the fabrication of bioceramics composed of silica and titanium oxide with various concentrations of titanium oxide for developing bone repair materials for dentistry and tissue engineering. The silica-based bioceramics were synthesized using the sol–gel method, and titanium oxide was added from the hydrolysis of tetrabutyl titanate. The surface morphology was observed using scanning electron microscopy. The chemical composition was measured using an energy dispersive X-ray spectrometer, and the crystal structure was identified by using an X-ray diffraction diffractometer. The pH value and ion concentrations released in simulated body fluids after immersion with bioceramic samples were measured using a pH meter and inductively coupled plasma mass spectrometry, respectively. In the cell toxicity test, the human osteosarcoma cells (MG63) were used and quantitatively assessed using an MTT assay. The results showed that the proposed bioceramics can be controlled by tuning the Si/Ti ratio to modify the dissolution rate of samples and enhance the formation of apatite. Compared to Dulbecco’s modified Eagle’s medium (DMEM) groups, the cell number of the BG_Ti75 group can be increased to 120%. Furthermore, BG_Ti75 can promote MG63 cell growth with statistical significance and keep the pH value and the released calcium ion concentrations of the soaking environment stable. The proposed bioceramics show potential for bone-regenerating capability.

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Involvement of bone morphogenetic protein–related pathways in the effect of aucubin on the promotion of osteoblast differentiation in MG63 cells

Cited by 29 publications

References 58 publications

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

In Vitro Properties for Bioceramics Composed of Silica and Titanium Oxide Composites

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Involvement of bone morphogenetic protein–related pathways in the effect of aucubin on the promotion of osteoblast differentiation in MG63 cells

Cited by 29 publications

References 58 publications

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

In Vitro Properties for Bioceramics Composed of Silica and Titanium Oxide Composites

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Involvement of bone morphogenetic protein–related pathways in the effect of aucubin on the promotion of osteoblast differentiation in MG63 cells