Involvement of BmoR and BmoG in n-alkane metabolism in ‘Pseudomonas butanovora’

Kurth, Elizabeth G.; Doughty, David M.; Bottomley, Peter J.; Arp, Daniel J.; Sayavedra-Soto, Luis A.

doi:10.1099/mic.0.2007/012724-0

Cited by 47 publications

(49 citation statements)

References 38 publications

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“…Induction of the alkane monooxygenase in P. butanovora is well studied. Alkane monooxygenase is not expressed during growth of P. butanovora on organic acids, but is induced during growth on C 2 -C 5 n-alkanes [137][138][139] and during the growth on alcohols and aldehydes that result from the oxidation of C 2 -C 9 n-alkanes [25,137,140,141]. The BMO structural genes are transcribed as a single polycistronic mRNA and the transcription of the cluster is controlled by a σ 54 promoter [131], identical to the consensus sequence of mmo genes in Methylosinus trichosporium OB3b, Methylocystis sp.…”

Section: Butane Monooxygenase (Bmo) In Pseudomonas Butanovoramentioning

confidence: 99%

“…BmoR is suggested to be necessary for the exponential growth on C 6 -C 8 n-alkanes [139]. Another gene (bmoG) at the 3'-end of the BMO operon encodes a peptide (BmoG) similar to GroEL chaperonins.…”

Section: Butane Monooxygenase (Bmo) In Pseudomonas Butanovoramentioning

confidence: 99%

“…Another gene (bmoG) at the 3'-end of the BMO operon encodes a peptide (BmoG) similar to GroEL chaperonins. BmoG is proposed to assist the proper assembly of BMO [139]. orf1 encodes a transcriptional regulator of the GntR family.…”

Section: Butane Monooxygenase (Bmo) In Pseudomonas Butanovoramentioning

confidence: 99%

“…orf1 encodes a transcriptional regulator of the GntR family. istA and istB genes were identified as an operon encoding a putative transposase based on the sequence similarity [139]. In a variety of other microorganisms short chain alkane monooxygenase enzymes were described to be induced during growth on alkanes, but not during growth on non-alkane substrates [62,66,137].…”

Section: Butane Monooxygenase (Bmo) In Pseudomonas Butanovoramentioning

confidence: 99%

“…The six structural genes cluster, Induction of the alkane monooxygenase in P. butanovora is well studied. Alkane monooxygenase is not expressed during growth of P. butanovora on organic acids, but is induced during growth on C 2 -C 5 n-alkanes [137][138][139] and during the growth on alcohols and aldehydes that result from the oxidation of C 2 -C 9 n-alkanes [25,137,140,141]. In a variety of other microorganisms short chain alkane monooxygenase enzymes were described to be induced during growth on alkanes, but not during growth on non-alkane substrates [62,66,137].…”

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confidence: 99%

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Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Cappelletti

Fedi

Honda

et al. 2010

Journal of Biotechnology

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Discussion 107 Summary 113Chapter 5 -Analysis of the alkB gene expression Summary 157Chapter 6 -Proteomic analysis of n-alkanes growth of Rhodococcus sp. BCP1 Chapter 1 -General Introduction PrefaceThe quality of life on Earth is tightly related with the overall quality of the environment [1]. During the last century it was commonly believed that the abundance of land and resources were unlimited and it was rarely acknowledged that the production, use, and disposal of hazardous substances had environmental and health effects [1][2][3]. Owing to this, human activity has deliberately or inadvertently released into waters and soil a variety of toxic compounds as refrigerants, paints, solvents, herbicides and pesticides that can cause considerable environmental pollution and human health problems as a result of their persistence, toxicity, and transformation into hazardous metabolites [4,5]. Very soon, however, it became clear how the carelessness and negligence in handling the industrial wastes and gas emissions had caused dramatic effect on the nature and on human health [1].Nowadays, the concern about the global warming and the climate changing induced the government regulatory agencies to establish procedures for assessing the environmental impact and health hazards of chemicals and for controlling/limiting their utilization.International efforts have been applied to remedy many contaminated sites all over the world, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use [5]. In response to public and government concern and because of the intriguing research problems presented, environmental scientists, biologists and chemists have been giving increased attention to identifying and determining the behavior and fate of organic compounds in natural ecosystems [5].Bioremediation procedures offer an economical and effective possibility to degrade or render innocuous toxic pollutants using natural biological activity [1]. By definition, bioremediation implies the use of living organisms, primarily microorganisms, to degrade the Since contaminant compounds are transformed by living organisms through reactions that take place as a part of their metabolic processes, environmental biotechnology focuses on the development of techniques to optimize environmental parameters to allow these metabolic pathways to proceed faster and, therefore, to improve the bio-degradation [1]. As a result, the removal of the contaminated soil can be necessary to let the biodegradation proceed in controlled sites (such as bioreactors). These ex situ approaches provide optimal conditions out one single step of the entire bio-degradation process. Hydrocarbons in the environmentHydrocarbons are energy-rich organic compounds consisting of carbon and hydrogen atoms and they can be saturated (only single C-H bounds) or unsaturated (one ore more double or triple C-H bounds). Alkanes are saturated hydrocarbons with the general formula C n H 2n+2 ; they can b...

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