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Twenty three bacterial isolates either pure or consortium were initially screened on the basis of their ability to degrade as well as dechlorinate 4 -chlorobenzoic acid (4-CBA). Based on comparative growth response, three pure isolates Pseudomonas putida GVS-4, Pseudomonas aeruginosa GVS-18 and Pseudomonas aeruginosa GWS-19 and a consortium SW-2 was fi nally selected for further fi studies. The enzyme studies performed with cell free extracts revealed that dehalogenase activity was substrate specifi c with maximum activity at 300 μgml fi -1 substrate concentration. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 4 -chlorobenzoic acid (4-CBA) is catabolized by ortho-ring cleavage pathway. The dehalogenase enzyme profi le showed single fi enzyme band in case of GVS-4 (Rm 0.76), GVS-18 (Rm 0.84), GWS -19 (Rm 0.85) and two bands in SW-2 (Rm 0.71 & 0.10).

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