Involvement of AtoSC two-component system in Escherichia coli flagellar regulon

Θεοδώρου, Μάμας; Theodorou, Evaggelos C.; Kyriakidis, Dimitrios A.

doi:10.1007/s00726-011-1140-7

Cited by 32 publications

(33 citation statements)

References 42 publications

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“…For biotechnological approaches that aim at production of 3HB or related compounds, ackA mutants might hence be preferred to other acetate mutants. The AtoCA system has been implicated in the control of flagella synthesis and motility (Oshima et al, 2002;Theodorou et al, 2012). The ackA mutation affects motility and biofilm formation (Prüß and Wolfe, 1994;Wolfe et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12

et al. 2020

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Acetate is a characteristic by-product of Escherichia coli K-12 growing in batch cultures with glucose, both under aerobic as well as anaerobic conditions. While the reason underlying aerobic acetate production is still under discussion, during anaerobic growth acetate production is important for ATP generation by substrate level phosphorylation. Under both conditions, acetate is produced by a pathway consisting of the enzyme phosphate acetyltransferase (Pta) producing acetyl-phosphate from acetyl-coenzyme A, and of the enzyme acetate kinase (AckA) producing acetate from acetyl-phosphate, a reaction that is coupled to the production of ATP. Mutants in the AckA-Pta pathway differ from each other in the potential to produce and accumulate acetyl-phosphate. In the publication at hand, we investigated different mutants in the acetate pathway, both under aerobic as well as anaerobic conditions. While under aerobic conditions only small changes in growth rate were observed, all acetate mutants showed severe reduction in growth rate and changes in the by-product pattern during anaerobic growth. The AckA − mutant showed the most severe growth defect. The glucose uptake rate and the ATP concentration were strongly reduced in this strain. This mutant exhibited also changes in gene expression. In this strain, the atoDAEB operon was significantly upregulated under anaerobic conditions hinting to the production of acetoacetate. During anaerobic growth, protein acetylation increased significantly in the ackA mutant. Acetylation of several enzymes of glycolysis and central metabolism, of aspartate carbamoyl transferase, methionine synthase, catalase and of proteins involved in translation was increased. Supplementation of methionine and uracil eliminated the additional growth defect of the ackA mutant. The data show that anaerobic, fermentative growth of mutants in the AckA-Pta pathway is reduced but still possible. Growth reduction can be explained by the lack of an important ATP generating pathway of mixed acid fermentation. An ackA deletion mutant is more severely impaired than pta or ackA-pta deletion mutants. This is most probably due to the production of acetyl-P in the ackA mutant, leading to increased protein acetylation.

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Section: Discussionmentioning

confidence: 99%

The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12

et al. 2020

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“…As a final 2CSTS in the context of regulation of flhDC transcription, AtoS/AtoC facilitates the response to acetoacetate, spermidine, and histamine via enhancing transcription of flhD and fliA (68). This 2CSTS may expand the range of nutrients that E. coli is able to utilize.…”

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confidence: 99%

Involvement of Two-Component Signaling on Bacterial Motility and Biofilm Development

Prüß

2017

J Bacteriol

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Two-component signaling is a specialized mechanism that bacteria use to respond to changes in their environment. Nonpathogenic strains of Escherichia coli K-12 harbor 30 histidine kinases and 32 response regulators, which form a network of regulation that integrates many other global regulators that do not follow the two-component signaling mechanism, as well as signals from central metabolism. The output of this network is a multitude of phenotypic changes in response to changes in the environment. Among these phenotypic changes, many twocomponent systems control motility and/or the formation of biofilm, sessile communities of bacteria that form on surfaces. Motility is the first reversible attachment phase of biofilm development, followed by a so-called swim or stick switch toward surface organelles that aid in the subsequent phases. In the mature biofilm, motility heterogeneity is generated by a combination of evolutionary and gene regulatory events.

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“…Importantly, the expression levels of atoS and atoC were increased in the rhizoplane compared with those in the rhizosphere. The AtoSC signal-transducing system is critical for flagellar synthesis and chemotaxis, and is essential for bacteria to efficiently migrate towards high concentrations of attractants, such as plant-derived simple carbon sources and amino acids, and away from repellents [51]. In contrast, more genes belonging to the category "Cell wall/membrane/envelope biogenesis" showed higher expression levels in the rhizosphere, and several genes involved in lipopolysaccharide (LPS) synthesis and export, including lpxD, lpxL, lpxB, kdsA, and lptB, were observed to be more actively expressed in the rhizosphere (Figures 3 and 4).…”

Section: Figurementioning

confidence: 99%

Mechanisms Underlying the Rhizosphere-To-Rhizoplane Enrichment of Cellvibrio Unveiled by Genome-Centric Metagenomics and Metatranscriptomics

Zhang

Wang

et al. 2020

Microorganisms

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Maintaining integrity of the plant cell walls is critical for plant health, however, our previous study showed that Cellvibrio, which is recognized by its robust ability to degrade plant cell walls, was enriched from the citrus rhizosphere to the rhizoplane (i.e., the root surface). Here we investigated the mechanisms underlying the rhizosphere-to-rhizoplane enrichment of Cellvibrio through genome-centric metagenomics and metatranscriptomics analyses. We recovered a near-complete metagenome-assembled genome representing a potentially novel species of Cellvibrio, herein designated Bin79, with genome size of 5.71 Mb across 11 scaffolds. Differential gene expression analysis demonstrated that plant cell wall degradation genes were repressed, whereas genes encoding chitin-degrading enzymes were induced in the rhizoplane compared with the rhizosphere. Enhanced expression of multi-drug efflux genes and iron acquisition- and storage-associated genes in the rhizoplane indicated mechanisms by which Bin79 competes with other microbes. In addition, genes involved in repelling plant immune responses were significantly activated in the rhizoplane. Comparative genomics analyses with five related Cellvibrio strains showed the importance of gene gain events for the rhizoplane adaptation of Bin79. Overall, this study characterizes a novel Cellvibrio strain and indicates the mechanisms involved in its adaptation to the rhizoplane from meta-omics data without cultivation.

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Involvement of AtoSC two-component system in Escherichia coli flagellar regulon

Cited by 32 publications

References 42 publications

The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12

The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12

Involvement of Two-Component Signaling on Bacterial Motility and Biofilm Development

Mechanisms Underlying the Rhizosphere-To-Rhizoplane Enrichment of Cellvibrio Unveiled by Genome-Centric Metagenomics and Metatranscriptomics

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