Involvement of an efflux transporter in prochloraz resistance of Fusarium fujikuroi CF245 causing rice bakanae disease

Yang, You Ri; Kim, Young Cheol; Lee, Se Won; Lee, Si Woo; An, Gwang Guk; Kim, In Soo

doi:10.1007/s13765-012-2126-1

Cited by 14 publications

(10 citation statements)

References 9 publications

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“…In nursery box settings without pathogen inoculation, the reduction of bakanae severity at the higher treatment concentrations was confirmed with control values of 60.0–66.7%, which was not significantly different from that of the chemical seed treatment with both prochloraz and fludioxonil ( Table 2 ). Seed treatment with only prochloraz almost did not induce the suppression of bakanae; this appeared to be due to the fungicide resistance developed in F. fujikuroi ( Yang et al, 2012 ). In control seedlings, high disease severity was shown in both pot and nursery box tests, which indicated severe infection of rice seeds by the pathogen during cultivation in fields ( Kazempour and Elahinia, 2007 ; Kim, 1981 ).…”

Section: Discussionmentioning

confidence: 94%

“…Seed treatments with the fungicides have been used widely in most rice growing areas during the last decades. However, due to the occurrence of increasing fungal resistance to these chemicals, the efficacy of these chemicals has been greatly reduced ( Park et al, 2009 ; Yang et al, 2012 ). Seed soaking in hot water (50–55°C) or salty hot water has been used to control seed-borne diseases including bakanae in some areas, but it is not commonly practiced ( Bonman, 1992 ; Tung and Serrano, 2011 ).…”

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confidence: 99%

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Biological Control of Rice Bakanae by an Endophytic Bacillus oryzicola YC7007

Hossain

Khan

Chung

et al. 2016

The Plant Pathology Journal

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In our previous study, we reported that a novel endophytic bacterium Bacillus oryzicola YC7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. This endophytic strain, B. oryzicola YC7007 was used as a biological control agent against bakanae disease of rice caused by Fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. Root drenching with B. oryzicola YC7007 suspension reduced the disease severity of bakanae significantly when compared with the untreated controls. The treatments of B. oryzicola YC7007 suspension (2.0 × 107 cfu/ml) to the rice rhizosphere reduced bakanae severity by 46–78% in pots and nursery box tests containing autoclaved and non-autoclaved soils. Moreover, in the detached rice leaves bioassay, the development of necrotic lesion and mycelial expansion of F. fujikuroi were inhibited significantly by spraying the culture filtrate of B. oryzicola YC7007. Drenching of ethyl acetate extracts of the culture filtrate to the rhizosphere of rice seedlings also reduced the bakanae disease severity in the plant culture dish tests. With the root drenching of B. oryzicola YC7007 suspension, the accumulation of hydrogen peroxide was observed at an early stage of rice seedlings, and a hormonal defense was elicited with and without pathogen inoculation. Our results showed that the strain B. oryzicola YC7007 had a good biocontrol activity against the bakanae disease of rice by direct inhibition, and was also capable of inducing systemic resistance against the pathogen via primed induction of the jasmonic acid pathway.

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Section: Discussionmentioning

confidence: 94%

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confidence: 99%

Biological Control of Rice Bakanae by an Endophytic Bacillus oryzicola YC7007

Hossain

Khan

Chung

et al. 2016

The Plant Pathology Journal

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“…The yield losses due to bakanae caused by Fusarium fujikuroi were estimated to be approximately 10–50% in the Asian rice-growing areas ( Bonman, 1992 ). For controlling the diseases, chemical fungicides have been widely used in most Asian countries during past decades, but the efficacy of fungicides has decreased recently due to the occurrence of resistance ( Yang et al, 2012 ). Additionally, the misuse of chemical fungicides has adverse effects on the agricultural environment and farmers, so the development of alternative control measures, specifically, biological controls using antagonistic microorganisms, has been attempted in many Asian countries ( Gnanamanickam, 2009 ).…”

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confidence: 99%

Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice

Chung

Hossain

Khan

et al. 2015

The Plant Pathology Journal

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Biological control of major rice diseases has been attempted in several rice-growing countries in Asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. Two novel endophytic Bacillus species, designated strains YC7007 and YC7010T, with anti-microbial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at Jinju, Korea, and their multifunctional activities were analyzed. Strain YC7007 inhibited mycelial growth of major rice fungal pathogens strongly in vitro. Bacterial blight and panicle blight caused by Xanthomonas oryzae pv. oryzae (KACC 10208) and Burkholderia glumae (KACC 44022), respectively, were also suppressed effectively by drenching a bacterial suspension (107 cfu/ml) of strain YC7007 on the rhizosphere of rice. Additionally, strain YC7007 promoted the growth of rice seedlings with higher germination rates and more tillers than the untreated control. The taxonomic position of the strains was also investigated. Phylogenetic analyses based on 16S rRNA gene sequences indicated that both strains belong to the genus Bacillus, with high similarity to the closely related strains, Bacillus siamensis KACC 15859T (99.67%), Bacillus methylotrophicus KACC 13105T (99.65%), Bacillus amyloliquefaciens subsp. plantarum KACC 17177T (99.60%), and Bacillus tequilensis KACC 15944T (99.45%). The DNA-DNA relatedness value between strain YC7010T and the most closely related strain, B. siamensis KACC 15859T was 50.4±3.5%, but it was 91.5±11.0% between two strains YC7007 and YC7010T, indicating the same species. The major fatty acids of two strains were anteiso-C15:0 and iso C15:0. Both strains contained MK-7 as a major respiratory quinone system. The G+C contents of the genomic DNA of two strains were 50.5 mol% and 51.2 mol%, respectively. Based on these polyphasic studies, the two strains YC7007 and YC7010T represent novel species of the genus Bacillus, for which the name Bacillus oryzicola sp. nov. is proposed. The type strain is YC7010T (= KACC 18228T). Taken together, our findings suggest that novel endophytic Bacillus strains can be used for the biological control of rice diseases.

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“…In addition, involvement of the efflux transporter concerning prochloraz resistance has been reported. 21) Ammaf et al 22) reported the identification of the efflux transporter of F. graminearum, and it played an important role in both sensitivity to DMI and virulence. Recently, Hellin et al 23) reported that the overexpression of transporter genes accounts for the adaptation of DMI fungicides in F. culmorum.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism and expression level of <i>CYP51</i> (cytochrome P450) and sensitivity to ipconazole in <i>Fusarium fujikuroi</i> isolates

Tateishi

Miyake

Suga

2019

Journal of Pesticide Science

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The relationship between the nucleotide sequences of CYP51, its expression level and its sensitivity to ipconazole of Fusarium fujikuroi isolates were investigated. Single nucleotide polymorphisms (SNPs) were observed in the CYP51 of isolates with different sensitivities to ipconazole, but no amino acid substitution was detected in the putative amino acid sequence of the CYP51 protein. On the other hand, the expression of CYP51 was enhanced by the presence of ipconazole, and it tended to be higher in isolates with lower sensitivities and no gibberellin productivity. In the presumed promoter region, the upstream nucleotide sequence of CYP51, several common SNPs and insertions of nucleotides were detected in the lower sensitivity isolates. These results suggest that F. fujikuroi isolates consist of 2 different groups in sensitivity and gibberellin productivity, and no amino acid substitution in CYP51 protein may contribute to the stably high efficacy of ipconazole against rice Bakanae disease for more than 25 years.

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Involvement of an efflux transporter in prochloraz resistance of Fusarium fujikuroi CF245 causing rice bakanae disease

Cited by 14 publications

References 9 publications

Biological Control of Rice Bakanae by an Endophytic Bacillus oryzicola YC7007

Biological Control of Rice Bakanae by an Endophytic Bacillus oryzicola YC7007

Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice

Polymorphism and expression level of <i>CYP51</i> (cytochrome P450) and sensitivity to ipconazole in <i>Fusarium fujikuroi</i> isolates

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