Polymorphism and expression level of &lt;i&gt;CYP51&lt;/i&gt; (cytochrome P450) and sensitivity to ipconazole in &lt;i&gt;Fusarium fujikuroi&lt;/i&gt; isolates

Tateishi, Hideaki; Miyake, Taiji; Suga, Haruhisa

doi:10.1584/jpestics.d18-011

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…Type I resistant isolates (half of the resistant isolates) were found with no insertions in the promoter region, consistent with results from Sclerotinia homoeocarpa 41 and Fusarium fujikuroi. 42 Type II resistant isolates (the other half of the resistant isolates) were observed to contain a 6 bp insertion in the promoter region, which may be associated with DMI resistance but needs further verification. However, it is unknown whether transcription factors 43 or CYP51 copies 44 contribute to the over-expression of CYP51 in our resistant isolates.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, we observed that induced expression of CYP51 was associated with DMI resistance in all resistant isolates with two genotypes. Type I resistant isolates (half of the resistant isolates) were found with no insertions in the promoter region, consistent with results from Sclerotinia homoeocarpa and Fusarium fujikuroi . Type II resistant isolates (the other half of the resistant isolates) were observed to contain a 6 bp insertion in the promoter region, which may be associated with DMI resistance but needs further verification.…”

Section: Discussionmentioning

confidence: 99%

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Induced expression of CYP51 associated with difenoconazole resistance in the pathogenic Alternaria sect. on potato in China

Zhang

Zhou

Tian

et al. 2019

Pest Management Science

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BACKGROUND Early blight caused by Alternaria spp. is amongst the most important diseases in potato. Demethylation inhibitor (DMI) fungicides are widely used to control the disease but long‐term use may decrease its control efficacy due to fungicide resistance. This study investigated the occurrence of difenoconazole resistance in Alternaria spp. and molecular resistant mechanisms. RESULTS EC50 values of 160 isolates to difenoconazole ranged from 0.026 μg mL−1 to 15.506 μg mL−1 and the frequency of difenoconazole sensitivity formed a non‐normal distribution curve with a major and a minor peak. Isolates with EC50 values of 4.121 and 5.461 μg mL−1 were not controlled effectively at fungicide doses of 50 and 100 μg mL−1. Cross‐resistance was observed between DMI fungicides difenoconazole and propiconazole, but not between difenoconazole and other fungicide groups, including boscalid, iprodione, or carbendazim. The CYP51gene was 1673 bp encoding 525 amino acids in length and contained two introns. All sensitive and resistant isolates had the identical amino acid sequence of CYP51, with the exception of one resistant isolate carrying a mutation of R511W. A 6 bp insertion in the upstream region was observed in half of the resistant isolates. In the absence of propiconazole, the relative expression of CYP51 was not significantly different in sensitive and resistant isolates. In the presence of difenoconazole, expression of CYP51 gene was induced significantly in the DMI‐resistant isolates but not in the sensitive ones. CONCLUSION Induced expression of CYP51 in resistant isolates exposed to difenoconazole is an important determinant for DMI resistance in potato pathogens Alternaria sect. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Induced expression of CYP51 associated with difenoconazole resistance in the pathogenic Alternaria sect. on potato in China

Zhang

Zhou

Tian

et al. 2019

Pest Management Science

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“…Inhibitors inhibited the activity of these enzymes and blocked the synthesis of ergosterol, which may disrupt the structure of the cell membrane and prevent infection and reproduction. CYP51 is an important enzyme in the biosynthetic process of ergosterol, and DMIs target CYP51 enzymes [ 59 ], e.g., CgCYP51A and CgCYP51B in C. gloeosporioides [ 60 ]. Here, we characterized the role of CsCyp51G1 in regulating the sensitivity of C. siamense to fludioxonil but not azoles.…”

Section: Discussionmentioning

confidence: 99%

The Transcription Factor CsAtf1 Negatively Regulates the Cytochrome P450 Gene CsCyp51G1 to Increase Fludioxonil Sensitivity in Colletotrichum siamense

Guan

Miao

et al. 2022

JoF

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Previous studies have shown that the high-osmolarity glycerol mitogen-activated protein kinase (HOG MAPK) signaling pathway and its downstream transcription factor CsAtf1 are involved in the regulation of fludioxonil sensitivity in C. siamense. However, the downstream target genes of CsAtf1 related to the fludioxonil stress response remain unclear. Here, we performed chromatin immunoprecipitation sequencing (ChIP-Seq) and high-throughput RNA-sequencing (RNA-Seq) to identify genome-wide potential CsAtf1 target genes. A total of 3809 significantly differentially expressed genes were predicted to be directly regulated by CsAtf1, including 24 cytochrome oxidase-related genes. Among them, a cytochrome P450-encoding gene, designated CsCyp51G1, was confirmed to be a target gene, and its transcriptional expression was negatively regulated by CsAtf1, as determined using an electrophoretic mobility shift assay (EMSA), a yeast one-hybrid (Y1H) assay, and quantitative real-time PCR (qRT-PCR). Moreover, the overexpression mutant CsCYP51G1 of C. siamense exhibited increased fludioxonil tolerance, and the CsCYP51G1 deletion mutant exhibited decreased fludioxonil resistance, which revealed that CsCyp51G1 is involved in fludioxonil sensitivity regulation in C. siamense. However, the cellular ergosterol content of the mutants was not consistent with the phenotype of fludioxonil sensitivity, which indicated that CsCyp51G1 regulates fludioxonil sensitivity by affecting factors other than the ergosterol level in C. siamense. In conclusion, our data indicate that the transcription factor CsAtf1 negatively regulates the cytochrome P450 gene CsCyp51G1 to increase fludioxonil sensitivity in C. siamense.

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“…G-group/bakanae type strains produce GAs but no or low FUM, while F-group/stunt type strains produce FUM but no or low levels of GAs [22,23]. Interestingly, different sensitivities to the fungicides thiophanate-methyl [22] and ipconazole [24] were observed between the G-group and F-group strains. Despite the development of management strategies, F. fujikuroi still causes bakanae disease worldwide.…”

Section: Introductionmentioning

confidence: 94%

Various Methods for Controlling the <i>Bakanae</i> Disease in Rice

Suga

2021

RAS

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Bakanae disease, caused by Fusarium fujikuroi, is a serious problem in rice production. This disease is widespread across the world and leads to substantial yield losses. F. fujikuroi is known to produce various secondary metabolites, including the plant hormone gibberellin, which induces typical bakanae symptoms. In this article, authors overviewed the methods for controlling bakanae disease, including the use of host resistance, chemical compounds, biocontrol agents, natural products, and physical methods. Although various strategies have been applied to control bakanae disease, the disease is not yet completely prevented. Authors discuss the advantages and disadvantages of these various methods. In addition, the mode of action of major fungicides and the resistance mechanisms to these fungicides were outlined. These information contribute to the development of more effective methods for controlling bakanae disease.

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Polymorphism and expression level of <i>CYP51</i> (cytochrome P450) and sensitivity to ipconazole in <i>Fusarium fujikuroi</i> isolates

Cited by 3 publications

References 24 publications

Induced expression of CYP51 associated with difenoconazole resistance in the pathogenic Alternaria sect. on potato in China

Induced expression of CYP51 associated with difenoconazole resistance in the pathogenic Alternaria sect. on potato in China

The Transcription Factor CsAtf1 Negatively Regulates the Cytochrome P450 Gene CsCyp51G1 to Increase Fludioxonil Sensitivity in Colletotrichum siamense

Various Methods for Controlling the <i>Bakanae</i> Disease in Rice

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