Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against β-amyloid-induced alterations in red blood cells

Carelli-Alinovi, Cristiana; Ficarra, Silvana; Russo, Anna María; Giunta, Elena; Barreca, Davide; Galtieri, Antonio; Misiti, Francesco; Tellone, Ester

doi:10.1016/j.biochi.2015.11.022

Cited by 32 publications

(19 citation statements)

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“…These altered molecules could potentially be applied as biomarkers for detection of early AD. In erythrocytes, similarly as in neurons, an increase of Aβ toxicity is mediated by its binding with dimeric acetylcholinesterase AChE forms located on the RBCs external face [75]. External acetylcholinesterase conformational status is dependent on NO metabolism, involving PKC activity [75].…”

Section: Aβ-induced Morphological Changes In Erythrocytesmentioning

confidence: 99%

Section: Aβ-induced Morphological Changes In Erythrocytesmentioning

confidence: 99%

“…In erythrocytes, similarly as in neurons, an increase of Aβ toxicity is mediated by its binding with dimeric acetylcholinesterase AChE forms located on the RBCs external face [75]. External acetylcholinesterase conformational status is dependent on NO metabolism, involving PKC activity [75]. It was demonstrated that in RBCs PKC, in consideration of its known crucial role in RBC signalling, could play a key role in Aβ-induced oxidative imbalance [75].…”

mentioning

confidence: 99%

“…External acetylcholinesterase conformational status is dependent on NO metabolism, involving PKC activity [75]. It was demonstrated that in RBCs PKC, in consideration of its known crucial role in RBC signalling, could play a key role in Aβ-induced oxidative imbalance [75].An important aspect in RBC functioning is energy production, related closely with redox status. Indeed, in AD erythrocytes increased oxidative stress is manifested in the elevated concentrations of hydrogen peroxide and decreased glutathione/glutathione disulfide ratio and glutathione transferase activity [76].…”

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confidence: 99%

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Search for Alzheimer's Disease Biomarkers in Blood Cells: Hypotheses-Driven Approach

et al. 2017

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Current Alzheimer's disease (AD) diagnostics is based on cognitive testing, and detecting amyloid Aβ and τ pathology by brain imaging and assays of cerebrospinal fluid. However, biomarkers identifying complex pathways contributing to pathology are lacking, especially for early AD. Preferably, such biomarkers should be more cost-effective and present in easily available diagnostic tissues, such as blood. Here, we summarize the recent findings of potential early AD molecular diagnostic biomarkers in blood platelets, lymphocytes and erythrocytes. We review molecular alterations which refer to such main hypotheses of AD pathogenesis as amyloid cascade, oxidative and mitochondrial stress, inflammation and alterations in cell cycle regulatory molecules. The major advantage of such biomarkers is the potential ability to indicate individualized therapies in AD patients. Alzheimer's disease (AD) is the most prevalent age-related dementia worldwide and one of the major unmet, increasing medical and economic problems of the modern world. As of 2015, there were an estimated 46.8 million people with dementia worldwide. This number will increase to an estimated 74.7 million in 2030 [1].Among the neuropathological hallmarks of AD are: the progressive loss of brain neurons, synaptic degeneration and the deposition of extracellular amyloid plaques and intracellular neurofibrillary tangles (NFTs) in such regions of the brain as the hippocampus, cortex and amygdala. Amyloid plaques consist mainly of 40-and 42-amino-acid Aβ peptides (Aβ40 and Aβ42). Peptides are proteolytic cleavage products of the Aβ protein precursor (APP) but with no fully elucidated biological function. NFTs are deposits of paired helical filaments composed mainly of hyperphosphorylated τ protein, a microtubule-stabilizing protein that maintains neuronal cell structure and axonal transport. Nevertheless, neither senile plaques nor NFTs are absolute hallmarks of AD dementia, because cognitively intact aged individuals may exhibit both pathological changes upon postmortem brain examination or as assessed by positron emission tomography amyloid and τ imaging agents (amyloid-PET and τ-PET) [2][3][4][5].Clinical AD manifests by aggravating dysfunction and weakening functional cognitive processes, linked to brain neuron loss that is both progressive and permanent [4], and that within several years leads to total dependence on the caregiver and eventually to death. Advances in our knowledge of AD have shown that clinical symptoms usually develop after a long preclinical pathogenic process, lasting for decades, making early AD detection in asymptomatic patients a subject of great interest [4,5]. Increasing proof demonstrates that the therapeutic methods' effectiveness is strictly contingent on the early diagnosis of AD, before the occurrence of massive neuron loss and dementia. This highlights the key importance of biochemical biomarkers for early AD diagnostics. AD diagnostics & demand for novel, improved biomarkersThe broadly accepted recommendations for AD diagnosis...

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Section: Aβ-induced Morphological Changes In Erythrocytesmentioning

confidence: 99%

Section: Aβ-induced Morphological Changes In Erythrocytesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Search for Alzheimer's Disease Biomarkers in Blood Cells: Hypotheses-Driven Approach

et al. 2017

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“…The evaluation of the levels of erythrocytary hemolysis and met-Hb, membrane peroxidation, determination of the total reducing power (FRAP) and dosage of acetylcholinesterase on erythrocyte membrane were carried out according to Ficarra et al (2013, 2016), Scala et al (2015) and Carelli-Alinovi et al (2016). Nitrites and nitrates (as an index of stability of the phospholipidic bilayer), quantization of the total thiols and gluthathione (index of the cells redox state and onset of oxidative injuries) were performed utilizing a commercial determination kit supplied by Sigma Aldrich.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Study on the In vitro and In vivo Effects of Asparagopsis taxiformis Bioactive Phycoderivates on Teleosts

Marino

Gugliandolo

et al. 2016

Front. Physiol.

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Several compounds from marine organisms have been studied for their potential use in aquaculture. Among the red algae, Asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. Here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. Depending on the seasonal period of sampling, ethanol extracts of A. taxiformis exhibited significantly different inhibitory activity against fish pathogenic bacteria. The extract obtained in late spring showed strong antibacterial activity against Aeromonas salmonicida subsp. salmonicida, Vibrio alginolyticus, and V. vulnificus, and moderate activity against Photobacterium damselae subsp. damselae, P. damselae subsp. piscicida, V. harveyi and V. parahaemolyticus. Sea bass and gilthead sea bream were fed with pellets supplied with the alga and algal extracts. The absence of undesired effects on fish was demonstrated. Hematological and biochemical investigations allowed to confirm that the whole alga and its extracts could be proposed for a future application in aquaculture.

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Encapsulation of caffeine in polysaccharide oil-core nanocapsules

Milkova

Goycoolea

2020

Colloid Polym Sci

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Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against β-amyloid-induced alterations in red blood cells

Cited by 32 publications

References 45 publications

Search for Alzheimer's Disease Biomarkers in Blood Cells: Hypotheses-Driven Approach

Search for Alzheimer's Disease Biomarkers in Blood Cells: Hypotheses-Driven Approach

Preliminary Study on the In vitro and In vivo Effects of Asparagopsis taxiformis Bioactive Phycoderivates on Teleosts

Encapsulation of caffeine in polysaccharide oil-core nanocapsules

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