Investigations on the Stability of Stevioside and Rebaudioside A in Soft Drinks

Abstract

The stability of the two steviol glycosides stevioside and rebaudioside A and the possible formation of the aglycon steviol in different soft drinks were analyzed in samples spiked with stevioside or rebaudioside A after 24, 48, and 72 h storage times at 80 °C. Degradation of up to 70% was observed, and stevioside was less stable than rebaudioside A. Stevioside and rebaudioside A and their degradation products were analyzed by high-performance liquid chromatography with ultraviolet detection (UV-HPLC) on a HILIC analytical column, and the identity of the degradation products was confirmed by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS(n)) in negative mode. A UV-HPLC method was developed using a C18 analytical column to exclude the presence of the aglycon steviol, which gave a positive response in the forward mutation assay using the sensitive Salmonella typhimurium TM677 strain. The recoveries of steviol with this method ranged from 95.9 to 109.2%, and the calibration curves were linear from 1 to 100 μg/mL with R(2) = 0.9999. The limit of detection was 1 μg/mL. Confirmation by LC-ESI-MS(n) resulted in a LOD of 6 ng/mL. The absence of steviol in the degraded samples could be unambiguously confirmed by UV-HPLC and by LC-ESI-MS(n).

The Effects of Rebaudioside A on Microbial Diversity in Mouse Intestine

“…RA has been a focus for decades, its structure (Steinmetz and Lin, 2009;Upreti et al, 2012), pharmacokinetic (Wheeler et al, 2008), stability properties WÖlwer-Rieck et al, 2010), clinical safety (Hsieh et al, 2003), and toxicologies, including chronic toxicity Nikiforov and Eapen, 2008), genotoxicity (Williams and Burdock, 2009), carcinogenicity (Toyoda et al, 1997;Xili et al, 1992), reproductive and developmental toxicity had been fully studied. In addition, some beneficial functions of RA, including nontoxicological effects, antioxidant, antihypertensive, anti-Inflammatory and immunomodulatory activities, and antihyperglycemic activities had also been proved (Boonkaewwan et al, 2006;Chan et al, 2000;Gregersen et al, 2004;Jeppesen et al, 2002;Lee et al, 2001;Tadhani et al, 2007).…”

Recent Analytical Methods for the Analysis of Sweeteners in Food: A Regulatory Perspective

“…Steviol glycosides under certain conditions are considered Generally Recognized as Safe (GRAS) by the FDA and are approved in the EU. Stevia produces several diterpene glycosides, the most abundant being STV and REB A [13]. Five other steviol glycosides have been identifi ed as minor components of the stevia leaf, including Reb fi C, D, F, dulcoside A, and rubusoside.…”

Preparative separation of steviol glycosides fromStevia rebaudianabertoni by macroporous resin and preparative HPLC

“…To the best of our knowledge, a few papers have reported on the use of analytical HILIC for the analysis and determination of steviol glycosides in the plant extracts or other else. Ursula Woelwer-Rieck et al applied analytical HILIC column to analyze stevioside and rebaudioside A and their degradation products from different soft drinks [20]. Ursula Woelwer-Rieck et al performed on a Luna HILIC analytical column to the analysis of stevioside and rebaudioside A [21].…”