Investigations on the pancreatic and stomach secretion in pigs by means of continuous infusion of<sup>14</sup>C-amino acids

Simon, O.; Żebrowska, T.; Bergner, H.; Münchmeyer, R

doi:10.1080/17450398309425668

Cited by 39 publications

(12 citation statements)

References 14 publications

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“…It has been reported that up to 25% of the daily protein synthesis in the body may be secreted into the gut (Simon et al., 1983). Endogenous nitrogen consists mainly of nitrogen from digestive enzymes, mucoproteins, desquamated cells, serum albumin, peptides, free amino acids and amines (Moughan and Schuttert, 1991).…”

Section: Introductionmentioning

confidence: 99%

ORIGINAL ARTICLE: Ileal endogenous amino acid flow of broiler chickens under high ambient temperature

Soleimani

Kasim

Alimon

et al. 2010

Journal of Animal Physiology and Animal Nutrition

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Summary High environmental temperature has detrimental effects on the gastrointestinal tract of poultry. An experiment was conducted to determine the effect of acute heat stress on endogenous amino acid (EAA) flow in broiler chickens. A total of 90, day‐old broiler chicks were housed in battery cages in an environmentally controlled chamber. Chicks were fed a nitrogen‐free diet on day 42 following either no heat exposure (no‐heat) or 2 weeks exposure to 35 ± 1 °C for 3 h from days 28 to 42 (2‐week heat) or 1 week exposure to 35 ± 1 °C for 3 h from days 35 to 42 (1 week heat). The most abundant amino acid in the ileal flow was glutamic acid, followed by aspartic acid, serine and threonine in non‐heat stressed group. The EAA flow in 1‐week heat and 2‐week heat birds were significantly (p < 0.05) higher than those under no heat exposure (14682, 11161 and 9597 mg/kg of dry matter intake respectively). Moreover, the EAA flow of 2‐week heat group was less than 1‐week heat group by approximately 36%. These observations suggest that the effect of heat stress on EAA flow is mostly quantitative; however, heat stress may also alter the content of EAA flow qualitatively.

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Section: Introductionmentioning

confidence: 99%

ORIGINAL ARTICLE: Ileal endogenous amino acid flow of broiler chickens under high ambient temperature

Soleimani

Kasim

Alimon

et al. 2010

Journal of Animal Physiology and Animal Nutrition

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“…Taking into account that both extracellular amino acids originating from plasma or digesta and intracellular amino acids derived from intracellular protein degradation are used for protein synthesis (Simon et al, 1982(Simon et al, , 1983 and that endogenous N in the gut lumen is derived from multiple precursor pools (Moughan et al, 1992), the ,5 N-enrichment in urinary N was used as an indicator of that in endogenous N during a steady state after withdrawal of 15 NH 4 C1. At this time, the 15 N in urine originated only from the catabolism of 15 N-labelled body protein.…”

Section: Methodical Aspectsmentioning

confidence: 99%

Determination of endogenous nitrogen associated with bacteria in ileal digesta of pigs receiving cereal-based diets with or without fish meal and various fibre supplements by using a simple <sup>15</sup>N-dilution technique

Bartelt¹,

Drochner²,

Götz³

et al. 1999

J. Anim. Feed Sci.

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Four Gottinger miniature pigs and five domestic pigs (Landrace) of similar body weight fitted with an ileocaecal re-entrant cannula were given each a cereal-based diets with or without fish meal (Treatments 1 and 2). The diets contained increasing levels of partially hydrolysed straw meal and pectin (2:1 w/w). On day 3, 5 and 7 after the last administration of 15 NH 4 C1 given with the diets during five days the ileal flow of endogenous nitrogen (N) was measured using the atom % 15 N excess in urinary N as indicator for that in endogenous N. The 15 N-enrichments in urinary N, in trichloroacetic acid (TCA)-soluble N of blood plasma as well as in TCA-soluble and -preciptable N in the pancreas and small intestine were nearly the same. Furthermore, the contribution of endogenous N to total, TCA-preciptable and bacterial N of ileal digesta was not affected by the three collection periods. Urinary N seems to be the easiest accessible and valid indicator for determination of the endogenous N under these experimental conditions. Neither the fibre supplements nor the

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“…The continuous-infusion method (Waterlow et al 1978 a) requires several hours to reach a plateau of constant labelling, even in the rat, and has largely been replaced by giving a flooding dose of tracer amino acid, which produces uniform labelling of free amino acid over 10-20 min (Kean, 1959;Scornik & Botbol, 1976;McNurlan, Tomkins & Garlick, 1979;Garlick, McNurlan & Preedy, 1980 c). The method is particularly useful for rapidly turning over tissues such as gut mucosa (McNurlan, Pain & Garlick, 1980) and pancreas (Simon, Zebrowska, Bergner & Munchmeyer, 1983). Because of the short time scale, the method measures the synthesis of both fixed and secreted proteins.…”

Section: Measurement Ofprotein Turnover Rates In Individual Tissuesmentioning

confidence: 99%

Protein Turnover With Special Reference to Man

1984

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This review is concerned with rates of N flux in the living animal rather than with mechanisms of protein synthesis and breakdown at the cellular level. Methods of measuring protein turnover in the whole body are discussed, with special emphasis on studies in man, and results obtained by different methods have been compared. Aspects of whole body protein turnover which are of physiological interest include its relation to body size, growth and development, energy metabolism and food intake. There are substantial increases in protein turnover in injury, and changes that occur in exercise are beginning to be explored. From the physiological point of view these results point the need for future research along two main lines. The first is that of regulation: a wide variety of hormones stimulate or repress protein synthesis and breakdown, with varying actions in different tissues. These effects, however, do not in themselves explain the mechanism by which a balance between synthesis and breakdown is maintained. Secondly, the fact that all cellular proteins are in a dynamic state poses questions about the relation between structure and function in tissues such as muscle and brain, which physiologists have hardly begun to tackle.

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Investigations on the pancreatic and stomach secretion in pigs by means of continuous infusion of¹⁴C-amino acids

Cited by 39 publications

References 14 publications

ORIGINAL ARTICLE: Ileal endogenous amino acid flow of broiler chickens under high ambient temperature

ORIGINAL ARTICLE: Ileal endogenous amino acid flow of broiler chickens under high ambient temperature

Determination of endogenous nitrogen associated with bacteria in ileal digesta of pigs receiving cereal-based diets with or without fish meal and various fibre supplements by using a simple <sup>15</sup>N-dilution technique

Protein Turnover With Special Reference to Man

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Investigations on the pancreatic and stomach secretion in pigs by means of continuous infusion of14C-amino acids

Cited by 39 publications

References 14 publications

ORIGINAL ARTICLE: Ileal endogenous amino acid flow of broiler chickens under high ambient temperature

ORIGINAL ARTICLE: Ileal endogenous amino acid flow of broiler chickens under high ambient temperature

Determination of endogenous nitrogen associated with bacteria in ileal digesta of pigs receiving cereal-based diets with or without fish meal and various fibre supplements by using a simple <sup>15</sup>N-dilution technique

Protein Turnover With Special Reference to Man

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Investigations on the pancreatic and stomach secretion in pigs by means of continuous infusion of¹⁴C-amino acids