Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract

Wang, Juan; Li, Lan Zhou; Liu, Yan Ge; Li, Teng; Lu, Jin; Xie, Jing; Hu, Wen Ji; Liu, Yang; Wang, Di; Teng, Le Sheng

doi:10.3892/mmr.2015.4734

Cited by 18 publications

(16 citation statements)

References 44 publications

(49 reference statements)

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“…Tramadol, a typical central antinociceptive medicine, strongly reduces pain in phase I, while peripherally active drugs selectively inhibit phase II responses (Rauf et al, 2015). In our previous experiment, we verified that PH shows no effects on autonomic activity and prolongs the residence time in a Rota-rod test (Wang et al, 2015). Collectively, the antinociceptive effects of PH mainly occur via the central nervous system.…”

Section: Discussionsupporting

confidence: 57%

Anti-nociceptive effects of Paecilomyces hepiali via multiple pathways in mouse models

Wang

Jia

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Paecilomyces hepiali (PH), a well-known medicinal fungus, has various pharmacological efficacies. In our study, the antinociceptive effects of PH and underlying mechanisms were evaluated using various mouse models. An acetic acid-induced writhing test, hot plate test, and formalin test were employed to evaluate the antinociceptive activities of PH. The levels of neuronal nitric oxide synthase (nNOS) in the hypothalamus and monoamine neurotransmitters in the serum and hypothalamus of experimental mice were examined. Additionally, hot plate tests using mice pretreated with various antagonists were used to determine the mechanisms of PHmediated antinociception. The PH-enhanced latency period of mice in the hot plate test was significantly blocked by pretreatment with atropine and glibenclamide. PH shortened the phase I and phase II reaction times of formalin-treated mice. Strongly reduced writhing and stretching induced by acetic acid were observed in PH-treated mice, indicating that PH mainly exerts antinociceptive activity on neurogenic pain. After thermal pain stimulation for 30 s, compared to control mice, 7-day PH-treated mice had lower nNOS and dopamine levels, and increased levels of serotonin in both the serum and hypothalamus. Collectively, our data showed that PH mediated antinociceptive activities via multiple pathways, including monoamines, nNOS/ATPsensitive K + channels, and M-type acetylcholine receptors.

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Section: Discussionsupporting

confidence: 57%

Anti-nociceptive effects of Paecilomyces hepiali via multiple pathways in mouse models

Wang

Jia

et al. 2016

Genet. Mol. Res.

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“…Compared to control mice, three-week AC treatment brought no significant differences in numbers of standing and activities indicating its safety on mice ( P > 0.05, F = 0.03–1.47; Figure 2(a)). FST, RRT, and ERT are classic rodent models to evaluate the capacity of antifatigue [24]. AC at doses of 0.3 and 0.9 g/kg strongly enhanced exercise capacity of mice in ERT and FST ( P < 0.05, Figures 2(b) and 2(c)).…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, AMPK positively replenishes cellular ATP supplies and suppresses the activation of mTOR to mediate ATP-consuming biosynthetic processes [43]. Gracilaria eucheumoides [7] and Paecilomyces hepiali [24] extract improved the ability to fight fatigue in mice via modulating the activation of Akt, mTOR, and AMPK. Similarly, AC significantly inhibited the phosphorylation of mTOR by upregulating the activation of AMPK and Akt in liver and skeletal muscle, which may play central roles during its enhancing exercise tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…Following previous description [24], thirty minutes after AC administration, mice were trained twice on the runway at 20 rpm for 5 min to adapt to the treadmill (FT-200, Chengdu Taimeng Science Technology Co., Ltd., Chengdu, China). Exhaustion was determined by failing to return to the runway within 15 sec and losing dynamism of movements.…”

Section: Methodsmentioning

confidence: 99%

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Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

Liu

et al. 2017

BioMed Research International

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Antrodia cinnamomea, a folk medicinal mushroom, has numerous biological effects. In this study, we aim to assess whether the antifatigue effects of A. cinnamomea mycelia (AC) and its underlying mechanisms are related to oxidative stress signaling using behavioral mouse models and biochemical indices detection. Mice were orally treated with AC at doses of 0.1, 0.3, and 0.9 g/kg for three weeks. AC had no effect on the spontaneous activities of mice indicating its safety on central nervous system. Furthermore, results obtained from weight-loaded forced swimming test, rotary rod test, and exhausted running test confirmed that AC significantly enhanced exercise tolerance of mice. Biochemical indices levels showed that these effects were closely correlated with inhibiting the depletion of glycogen and adenosine triphosphate stores, regulating oxidative stress-related parameters (superoxide dismutase, glutathione peroxidase, reactive oxygen species, and malondialdehyde) in serum, skeletal muscle, and liver of mice. Moreover, the effects of AC may be related with its regulation on the activations of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin in liver and skeletal muscle of mice. Altogether, our data suggest that the antifatigue properties of AC may be one such modulation mechanism via oxidative stress-related signaling in mice.

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“…Paecilomyces hepiali, a parasitic fungus generally found in C. sinensis, contains identical chemical constituents and similar bioactivities to C. sinensis (13). The authors previously confirmed various pharmacological activities of polysaccharide-enriching P. hepiali extract via regulating the oxidative system (14,15). However, its potential regulatory effect on depression-like rat models and molecular mechanism has not been reported yet.…”

Section: Introductionmentioning

confidence: 85%

Dopamine and serotonin contribute to Paecilomyces hepiali against chronic unpredictable mild stress induced depressive behavior in Sprague Dawley rats

Wang

Liu

et al. 2017

Molecular Medicine Reports

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Paecilomyces hepiali contains identical chemical constituents to Cordyceps sinensis, and it presents antidepressant‑like activity via regulating noradrenergic and dopaminergic systems. Behavioral despair depression models serve important roles in scientific screening and evaluation of antidepressants. The present study aims to investigate the antidepressant‑like activity of P. hepiali extract (PHC) in chronic unpredictable mild stress (CUMS)‑induced rat model of depression. Following four weeks of treatment, similar to fluoxetine at 3 mg/kg (positive drug), PHC at doses from 0.08 to 2.0 g/kg strongly increased sucrose preference and reduced the immobility time of depression‑like rats in forced swimming test. The hypo‑level of adrenocorticotropic hormone, noradrenaline and glucocorticoid receptor in serum and hypothalamus of depression‑like rats was enhanced by PHC. PHC normalized CUMS‑induced disorders of dihydroxyphenylacetic acid, dopamine, 5‑hydroxytryptamine (5‑HT) and 5‑hydroxyindoleacetic acid in serum and/or hypothalamus of depression‑like rats. Moreover, PHC enhanced the expression of tyrosine hydroxylase and reduced the levels of dopamine D2 receptor and 5‑HT2A receptor in hypothalamus. These results suggested that the antidepressant‑like effects of PHC in CUMS‑induced depression are associated with not only the modulation of dopamine, but also the regulation of 5-HT.

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Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract

Cited by 18 publications

References 44 publications

Anti-nociceptive effects of Paecilomyces hepiali via multiple pathways in mouse models

Anti-nociceptive effects of Paecilomyces hepiali via multiple pathways in mouse models

Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

Dopamine and serotonin contribute to Paecilomyces hepiali against chronic unpredictable mild stress induced depressive behavior in Sprague Dawley rats

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