Investigations of Urothelial Cells Seeded on Commercially Available Small Intestine Submucosa

Feil, G.; Christ-Adler, Margot; Maurer, Sabine; Corvin, Stefan; Rennekampff, Hans‐Oliver; Krug, Jutta; Hennenlotter, Jörg; Kuehs, Ursula; Stenzl, Arnulf; Sievert, Karl‐Dietrich

doi:10.1016/j.eururo.2006.05.041

Cited by 86 publications

(42 citation statements)

References 30 publications

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“…We found that the PLC extract did not have a cytotoxic effect on ADSCs in all tested concentrations, as opposed to commercially available SIS extract, which was also confirmed by other authors [37]. In this study, SIS was used as a control because it is the reference material for urinary bladder tissue engineering.…”

Section: Discussionsupporting

confidence: 87%

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

et al. 2014

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The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

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Section: Discussionsupporting

confidence: 87%

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

et al. 2014

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“…The preparation of natural matrices commonly involves a combination of physical methods to delaminate layers of tissue, followed by chemical and enzymatic methods to remove cell bodies from the remaining ECM [23]. Such decellularisation strategies, designed to limit the immunogenicity of the matrix, are not always entirely successful [24].…”

Section: Introductionmentioning

confidence: 99%

Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering

et al. 2007

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“…A certain cytotoxicity was noticed concerning the SIS matrix, in addition to its immunogenic nature. Indeed, a series of postoperative inflammatory reactions (Ho et al 2004) preceded the in vitro detection of porcine DNA residues on the commercially available SIS, by specific chromatin staining (Feil et al 2006). These last reports made on this model, confirmed it as an unfavorable substitute for vesical reconstruction.…”

Section: Bladdermentioning

confidence: 49%

Evolution in Tissue Engineering for the Lower Urinary Tract

Bouhout¹,

Ouellet²,

Perron³

et al. 2010

Tissue Engineering

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Investigations of Urothelial Cells Seeded on Commercially Available Small Intestine Submucosa

Cited by 86 publications

References 30 publications

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering

Evolution in Tissue Engineering for the Lower Urinary Tract

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