Investigations of the Production of Cephalosporin C by Acremonium chrysogenum

Tollnick, C.; Seidel, G.; Beyer, Michael; Schügerl, K.

doi:10.1007/b12439

Cited by 18 publications

(25 citation statements)

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“…The CPC has a weak antibacterial activity, but the modification of its side chain generates semisynthetic cephalosporins having diversified antibacterial activity. The mold is recently renamed as Acremonium chrysogenum (Brakhage, 1998;Demain and Zhang, 1998;Silva et al 1998;Schmitt et al 2004;Tollnick et al 2004). The beta-lactam antibiotics particularly penicillin and cephalosporins share approximately 65% of worldwide market of antibiotics (Elander, 2003).…”

Section: *Corresponding Authormentioning

confidence: 99%

Influence of medium constituents on the biosynthesis of cephalosporin-C

Nigan¹,

Verma²,

Kumar³

et al. 2007

Electron. J. Biotechnol.

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Acremonium chrysogenum NCIM 1069 was used for the biosynthesis of cephalosporin-C (CPC) in batch mode of cultivation. The effect of different medium constituents for better yield of CPC was thoroughly investigated. From the results of the fermentation, it was found that ammonium sulphate as inorganic nitrogen source and methionine at the concentration of 0.4% are most suitable for higher yield of antibiotic. The variation in the C/N ratio on the biosynthesis of CPC showed that a C/N ratio of 8.0 is most suitable for maximum production of CPC.

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Section: *Corresponding Authormentioning

confidence: 99%

Influence of medium constituents on the biosynthesis of cephalosporin-C

Nigan¹,

Verma²,

Kumar³

et al. 2007

Electron. J. Biotechnol.

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“…According to the increase of extracellular a-aminoadipic acid (a-AAA) concentration, it can be concluded that, there was remarkable a-AAA secretion because a-AAA did not exist in the basal medium. Accumulation of lysine in broth also indicated that some proportion of a-AAA was converted into lysine [10]. Corresponding intracellular level of these two amino acids held increasing trends with some degree.…”

Section: Resultsmentioning

confidence: 92%

“…Moreover, there were some reviews which have comprehensively summarized the published reports. Tollnick et al [10] laid more emphasis on the cultivation media, cultivation and its process control, morphology, mathematical modeling, and control. Whereas Schmitt et al [11] mainly focused on various regulatory levels of CPC biosynthesis, they summarized that precursor amino acids, as well as activation and repression on the transcription level and enzyme activity, played an important role in the regulation of the CPC biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil

Yang

Chu

et al. 2010

Bioprocess Biosyst Eng

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A metabolic network was constructed for the Acremonium chrysogenum cultivation fed with soybean oil. Metabolic flux analysis indicated that the shift from exponential growth to rapid cephalosporin C (CPC) formation was accompanied by 1.63- and 5-fold carbon flux enlargement in TCA cycle and glyoxylate by-pass, respectively. The flux via pentose phosphate pathway branch was little affected during the rapid CPC formation period; the contributory explanation was that 35.6% of NADPH was consumed in the dissimilation of fatty acids. Estimation of NADPH, ATP generation, and consumption demonstrated that, with soybean oil as carbon source in rapid CPC formation phase, the NADPH consumed in fatty acid catabolism was fourfold greater than that used in the CPC biosynthesis-relevant part; simultaneously, more than 90% energy spent was not directly related to the CPC formation. Therefore, the improvement of CPC production yield through optimization of the NADPH, ATP generation, and consumption was put forward.

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“…7). This is of special interest for the determination of growth media required for the synthesis of complex metabolites like antibiotics (Tollnick et al 2004). One focus could be the analysis of the different proposed growth media with respect to efficiency of the production of the antibiotic and the cost for the production of the metabolites used in the medium.…”

Section: Determining Minimal Mediamentioning

confidence: 99%

Can the whole be less than the sum of its parts? Pathway analysis in genome-scale metabolic networks using elementary flux patterns

Kaleta¹,

Figueiredo²,

Schuster³

2009

Genome Res.

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Elementary modes represent a valuable concept in the analysis of metabolic reaction networks. However, they can only be computed in medium-size systems, preventing application to genome-scale metabolic models. In consequence, the analysis is usually constrained to a specific part of the known metabolism, and the remaining system is modeled using abstractions like exchange fluxes and external species. As we show by the analysis of a model of the central metabolism of Escherichia coli that has been previously analyzed using elementary modes, the choice of these abstractions heavily impacts the pathways that are detected, and the results are biased by the knowledge of the metabolic capabilities of the network by the user. In order to circumvent these problems, we introduce the concept of elementary flux patterns, which explicitly takes into account possible steady-state fluxes through a genome-scale metabolic network when analyzing pathways through a subsystem. By being similar to elementary mode analysis, our concept now allows for the application of many elementarymode-based tools to genome-scale metabolic networks. We present an algorithm to compute elementary flux patterns and analyze a model of the tricarboxylic acid cycle and adjacent reactions in E. coli. Thus, we detect several pathways that can be used as alternative routes to some central metabolic pathways. Finally, we give an outlook on further applications like the computation of minimal media, the development of knockout strategies, and the analysis of combined genome-scale networks.

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Investigations of the Production of Cephalosporin C by Acremonium chrysogenum

Cited by 18 publications

References 0 publications

Influence of medium constituents on the biosynthesis of cephalosporin-C

Influence of medium constituents on the biosynthesis of cephalosporin-C

Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil

Can the whole be less than the sum of its parts? Pathway analysis in genome-scale metabolic networks using elementary flux patterns

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